A protocol for separation and isolation of small and/or large DNA fragments with high yield using CL4B Sepharose

Analytical Biochemistry

Volumn 317, Issue 2, 2003, Pages 284-287

  Datta, Kamal ^a   Neumann, Ronald D ^a   Winters, Thomas A ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

DNA FRAGMENT; HIGH YIELD;

CHEMISTRY;

DNA;

ANALYTIC METHOD; ARTICLE; CENTRIFUGATION; DNA BINDING; DNA PURIFICATION; ELECTROPHORESIS; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; QUANTITATIVE ANALYSIS; STANDARDIZATION;

EID: 0038634734     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00168-4     Document Type: Article

References (4)

1. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. USA. 76:1979;615-619.
2. Chen H.S., Chang H.T. Electrophoretic separation of small DNA fragments in the presence of electroosmotic flow using poly(ethylene oxide) solutions. Anal. Chem. 71:1999;2033-2036.
3. Ratel D., Dupre I., Allamane S., Berger F., Verna J.M., Benabid A.L., Wion D. An enzymatic procedure for the purification of DNA restriction fragments without gel electrophoresis and ethidium bromide staining. C. R. Acad. Sci. III. 323:2000;753-756.
4. McLoughlin D.M., O'Brien J., McManus J.J., Gorelov A.V., Dawson K.A. A simple and effective separation and purification procedure for DNA fragments using dodecyltrimethylammonium bromide. Bioseparation. 9:2000;307-313.