Noncovalent assembly of microhelix recognition by a class II tRNA synthetase

Journal of the American Chemical Society

Volumn 120, Issue 13, 1998, Pages 3269-3270

  Sardesai, Niranjan Y ^a,b   Schimmel, Paul ^b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0038619935     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja9742287     Document Type: Letter

References (31)

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23. 14 The reaction mixture was extracted thrice with 2-butanol to remove excess reagent (organic layer), and the RNA was ethanol precipitated from the aquaeous layer. The APRNA pellet was resuspended in 0.1 M triethylammonium acetate (TEAA) and further purified to homogeniety by reverse phase HPLC using a C-18 column and 0.1 M TEAA/acetonitrile gradient. The two diastereomers of APRNA were collected together and used without further diastereomeric resolution.
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27. 32P]AP-RNA was recovered from the gel by by electroelution followed by ethanol precipitation.
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