Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support - Clinical study

International Journal of Dermatology

Volumn 42, Issue 3, 2003, Pages 219-223

  Dvořánková, Barbora ^b   Holíková, Zuzana ^b   Vacík, Jiří ^b   Königová, Radana ^b   Kapounková, Zuzana ^b   Michálek, Jiří ^b   Přádný, Martin ^b   Smetana Jr , Karel ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN TYPE 4; CYTOKERATIN; LAMININ; MONOCLONAL ANTIBODY; POLYMER;

ARTICLE; BURN; CELL CULTURE; CLINICAL TRIAL; HUMAN; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; LANGERHANS CELL; SKIN DEFECT; SKIN GRAFT; TROPHIC ULCER; WOUND CLOSURE; ADULT; AGED; CELL TRANSPLANTATION; CHILD; EPIDERMIS; FEMALE; INFANT; MALE; MIDDLE AGED; SKIN ULCER; TISSUE ENGINEERING; TRANSPLANTATION; WOUND HEALING;

ADULT; AGED; AGED, 80 AND OVER; BURNS; CELL TRANSPLANTATION; CELLS, CULTURED; CHILD; EPIDERMIS; FEMALE; HUMANS; INFANT; KERATINOCYTES; MALE; MIDDLE AGED; POLYMERS; SKIN ULCER; TISSUE AND ORGAN HARVESTING; TISSUE ENGINEERING; WOUND HEALING;

EID: 0038615838     PISSN: 00119059     EISSN: 13654632     Source Type: Journal    
DOI: 10.1046/j.1365-4362.2003.01792.x     Document Type: Article

References (26)

1. Silver FH. Medical devices and tissue engineering: an integrated approach. In: Biomaterials. London: Chapman & Hall, 1994: 1-302.
2. Dantzer E, Braye FM. Reconstructive surgery using an artificial dermis (Integra): results with 39 grafts. Br J Plast Surg 2001; 54: 659-664.
3. Dantzer E, Queruel P, Salinier L, et al. Integra, a new surgical alternative for the treatment of massive burns. Clinical evaluation of acute and reconstructive surgery: 39 cases. Ann Chir Plast Esthet 2001; 46: 173-189.
4. Wainwright DJ. Use of an acellular allograft dermal matrix (AlloDerm) in the management of full-thickness burns. Burns 1995; 21: 243-248.
5. Lattari V, Jones LM, Varcelotti JR, et al. The use of a permanent dermal allograft in full-thickness burns of the hand and foot: a report of three cases. J Burn Care Rehabil 1997; 18: 147-155.
6. Tsai CC, Lin SD, Lai CS, Lin TM. The use of composite acellular allodermis-ultrathin autograft on joint area in major burn patients - one year follow up. Kaohsiung J Med Sci 1999; 15: 651-658.
7. Mansbridge J, Liu K, Patch R, et al. Three-dimensional fibroblast culture implant for the treatment of diabetic foot ulcers: metabolic activity and therapeutic range. Tissue Eng 1998; 4: 403-414.
8. Streit M, Braathen LR. Apligraf-a living human skin equivalent for the treatment of chronic wounds. Int J Artif Organs 2000; 23: 831-833.
9. Smetana K Jr, Dvořánková B, Labský J, et al. Grafting of human epidermal cells, presence and perspectives. Sbornik Lekarsky 2001; 102: 1-6.
10. Green H, Kehihde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 1979; 76: 5665-5668.
11. Berhod F, Damour O. In vitro reconstructed skin models for wound coverage in deep burns. Br J Dermatol 1997; 136: 809-816.
12. Jones PH. Isolation and characterization of human epidermal stem cells. Clin Sci 1996; 91: 141-146.
13. Watt FM. Influence of cell shape and adhesiveness on stratification and terminal differentiation of human keratinocytes in culture. J Cell Sci Suppl 1987; 8: 313-326.
14. Dvořánková B, Smetana K Jr, Vacík J, Jelínková M. Cultivation of keratinocytes on polyHEMA and their migration after inversion. Folia Biol (Prague) 1996; 42: 83-86.
15. Smetana K Jr, Dvořánková B, Jelínková M, et al. Phenotypic characterization of keratinocytes migrated from polymer support - in vitro study. J Mater Sci Mater Med 1997; 8: 587-590.
16. Dvořánková B, Smetana K Jr, Königová R, et al. Cultivation and grafting of human keratinocytes on a poly(hydroxyethyl methacrylate) support to the wound bed: a clinical study. Biomaterials 1998; 19: 141-146.
17. Harvima IT, Virnes S, Kauppinen L, et al. Cultured allogenic skin cells are effective in the treatment of chronic diabetic leg and foot ulcers. Acta Derm Venereal (Stockh) 1999; 79: 217-220.
18. Naughton G, Mansbridge J, Gentzkow G. A metabolically active human dermal replacement for the treatment of diabetic foot ulcers. Int J Artif Organs 1997; 21: 1203-1210.
19. Carsin H, Ainaud P, Le Bever H, et al. Cultured epithelial autografts in extensive burn coverage of severely traumatized patients: a five year single-center experience with 30 patients. Burns 2000; 26: 379-387.
20. Rouabhia M, Germain L, Bergeron J, Auger FA. Allogenic-syngenic cultured epithelia. Transplantation 1995; 59: 1229-1335.
21. Kondo S. The roles of keratinocyte-derived cytokines in the epidermis and their possible responses to UVA-irradiation. J Invest Dermatol Symp Proc 1999; 4: 177-183.
22. Pokorná E, Brož L, Veselý P, Matouš ková E. Y chromosome and vimentin used to trace the fate of allogenic keratinocytes delivered to the wound by the recombinant human/pig skin. Folia Biol (Praha) 2001; 47: 128-134.
23. Compton CC, Gill JM, Bradford DA, et al. Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting. Lab Invest 1989; 60: 600-612.
24. Régnier M, Staquet JM, Schmitt D, Schmidt R. Integration of Langerhans cells into pigmented reconstructed human epidermis. J Invest Dermatol 1997; 109: 510-512.
25. Leigh IM, Navsaria HA. Pre-confluent keratinocyte grafting: the future for cultured skin replacements. Burns 1998; 24: 591-593.
26. Villeneuve P, Hafner J, Prenosil JE, et al. A novel culturing and grafting system for the treatment of leg ulcers. Br J Dermatol 1998; 138: 849-851.