Novel method for continuous cell separation by density gradient centrifugation: Evaluation of a miniature separation column

Preparative Biochemistry and Biotechnology

Volumn 33, Issue 2, 2003, Pages 87-100

  Shiono, Hiroyuki ^a,b   Ito, Yoichiro ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b AICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

Buffy coat; Cell separation; Continuous flow seal less centrifuge; Density gradient centrifugation; Granulocyte; Lymphocyte; Monocyte; Percoll; Platelet; RBCs; WBCs

Indexed keywords

BLOOD CELL COUNT; CELL SEPARATION; CENTRIFUGATION, DENSITY GRADIENT; CHROMATOGRAPHY; ERYTHROCYTES; HUMANS; LEUKOCYTES; LYMPHOCYTES; MONOCYTES; NEUTROPHILS; POVIDONE; REPRODUCIBILITY OF RESULTS; SILICON DIOXIDE;

ANIMALIA; PHOCIDAE;

EID: 0038586253     PISSN: 10826068     EISSN: None     Source Type: Journal    
DOI: 10.1081/PB-120021434     Document Type: Article

References (5)

1. van Vlasselaer, P.; Palathumpat, V.C.; Strang, G.; Shapiro, M.H. New approaches in density gradient separation using colloidal silica gradients in the processing of human hematopoietic progenitor cells. In Cell Separation Method and Applications; Recktenwald, D., Radbruch, A., Eds.; Marcel Dekker: New York, 1997; 15-41.
2. Figdor, C.G.; Preijers, F.; Huibens, R.; Ruijsm, O.; de Witte, T.J.M.; Bont, W.S. Centrifugal elutriation: A powerful separation technique in cell biology, immunology, and hematology. In Cell Separation Methods and Applications; Recktenbwald, D., Radbruch, A., Eds.; Marcel Dekker: New York, 1997; 43-59.
3. Ito, Y.; Shinomiya, K. A new continuous-flow separation method based on cell density: principle, apparatus, and preliminary application to separation of human buffy coat. J. Clin. Aphe. 2001, 16, 186-191.
4. Ito, Y.; Suaudeau, J.; Bowman, R.L. New flow-through centrifuge without rotating seals applied to plasmapheresis. Science 1970, 189, 999-1000.
5. Ito, Y. Flow-Through Centrifuge Free of Rotating Seals Particularly for Plasmapheresis. US Patent 4,425,112, 1984.