Purification and characterization of acetylcholinesterase from the Lake Van fish (Chalcalburnus tarichii Pallas, 1811)

Preparative Biochemistry and Biotechnology

Volumn 33, Issue 2, 2003, Pages 137-145

  Aliriz, Serpil ^a   Turkoglu, Vedat ^a  

^a YUZUNCU YIL UNIVERSITY   (Turkey)

Author keywords

Acetylcholinesterase; Affinity chromatography; Purification; Van Lake Fish (Chalcalburnus tarichii P. 1811)

Indexed keywords

ACETYLCHOLINESTERASE; ANIMALS; CHROMATOGRAPHY, AFFINITY; CYPRINIDAE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTES; HYDROGEN-ION CONCENTRATION; TEMPERATURE;

CHALCALBURNUS;

EID: 0038586244     PISSN: 10826068     EISSN: None     Source Type: Journal    
DOI: 10.1081/PB-120021438     Document Type: Article

References (14)

1. Lehninger, A.L. Principles of Biochemistry; Worth Publisher, Inc.: New York, 1982; 222-223.
2. Nachmansohn, D.; Lededer, E. Chemical and molecular basis of nerve activity. Bull. Soc. Chem. Biol. 1939, 21, 797.
3. Pilz, W. Klin. Wschr. 1958, 36, 1017.
4. Brewer, G.J.; Sing, C.F. An Introduction to Izoenzymes Techniques; Academic Press, Inc.: New York, 1970; 207.
5. Hoz, D.L.D.; Raltson, J.S.; Rush, R.S.; Wolfe, A.D. A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase. Life Sci. 1986, 39, 195-199.
6. Raltston, J.S.; Rush, R.S.; Doctor, P.B.; Wolfe, A.D. Acetylcholinesterase from fetal bovine serum purification and characterisation of soluble G4 enzyme. J. Biol. Chem. 1985, 260 (7), 4312-4318.
7. Main, A.R.; Soucie, W.G.; Buxton, L.I.; Arinc, E. The purification of cholinesterase from horse serum. Biochem. J. 1974, 143, 733-744.
8. Bruns, K.; Casillas, E.D. Partial purification and characterization of an acetylcarnitine hydrolase from bovine epididymal spermatozoa. Arch. Biochem. Biophys. 1990, 277 (1), 1-7.
9. Ellman, G.L.; Courtney, K.D.; Andres, V. Jr.; Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961, 7, 88-95.
10. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantaties of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248.
11. 4. Nature 1970, 227, 680.
12. Bell, J.E.; Bell, E.T. Affinity Chromatography. Protein and Enzymes; Prentice-Hall, Inc.: New Jersey, 1988; 45-63.
13. Ulusu, N.N.; Kus, M.S.; Acan, L.; Tezcan, E.F. Intl. J. Biochem. Cell Biol. 1999, 31, 787.
14. Bilgi, C.; Unsal, I.; Ozer, N.; Erbil, M.K.; Karaca, L. Tr. J. Med. Sci. 1995, 27, 291.