Secondary losses via γ-lactam formation in hot electron capture dissociation: A missing link to complete de Novo sequencing of proteins?

Journal of the American Chemical Society

Volumn 125, Issue 22, 2003, Pages 6628-6629

  Kjeldsen, Frank ^a,b   Zubarev, Roman ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA LACTAM DERIVATIVE; PROTEIN DERIVATIVE;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; CHEMICAL REACTION; DISSOCIATION; ELECTRON CAPTURE DETECTION; MASS SPECTROMETRY; PROTEIN ANALYSIS; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; CASEINS; CATTLE; FOURIER ANALYSIS; GLYCOPROTEINS; LACTAMS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 0038547949     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja035042c     Document Type: Article

References (15)

1. Chang, Y.; Hang, H.; Kim, B.; Hall, S.; Settineri, T. Int. J. Mass. Spectrom. 2001, 209. 47.
2. Uttenweiler-Joseph, S.; Neubauer, G.; Christoforidis, S.; Zerial, M.; Wilm, M. Proteomics 2001, 1, 668.
3. Cagney, G.; Emili, A. Nature 2002, 20, 163.
4. Ge, Y.; Lawhorn, B. G.; ElNaggar, M.; Strauss, E.; Park, J.; Begley, T. P.; McLafferty, F. W. J. Am. Chem. Soc. 2002, 124, 672.
5. Hunt, D. F.; Yates, J. R.; Shabanowitz, J.; Winston, S.; Hauer, C. R. 1986, 83, 6233.
6. Summerfield, S. G.; Steen, H.; O'Malley, M.; Gaskell, S. J. Int. J. Mass Spectrom. 1999, 188, 95.
7. Reid, G. E.; Tichy, S. E.; Pérez, J.; O'Hair, R. A. J.; Simpson, R. J.; Kenttämaa, H. I. J. Am. Chem. Soc. 2001, 123, 1184.
8. Medzihradszky, K. F.; Burlingame, A. L. Methods: A Companion to Methods in Enzymology; Academic Press: San Diego, 1994; Vol. 6, p 284.
9. Kjeldsen, F.; Haselmann, K. F.; Budnik, B. A.; Jensen, F.; Zubarev, R. A. Chem. Phys. Lett. 2002, 356, 201.
10. Kjeldsen, F.; Haselmann, K. F.; Sørensen, E. S.; Zuberav, R. A. Anal. Chem. 2003, 75, 1267.
11. The full sequence except the identities of the two N-terminal Xle residues was obtained as follows. Nine ECD and three low-energy CAD mass spectra of five Arg-C peptides and seven Lys-C peptides gave cleavages of all 134 interresidue bonds. The total sequence obtained by this "shotgun" approach was aligned using seven overlaps between the peptides. As a final step, HECD was performed for Xle identification.
12. Johnson, R. S.; Martin, S. A.; Biemann, K.; Stults, J. T.; Watson, J. T. Anal. Chem. 1987, 59, 2621.
13. Zubarev, R. A.; Kelleher, N. L.; McLafferty, F. W. J. Am. Chem. Soc. 1998, 120, 3265.
14. Cooper, H. J.; Hudgins, R. R.; Håkansson, K.; Marshall, A. G. J. Am. Soc. Mass Spectrom. 2002, 13, 241.
15. Haselmann, K. F.; Budnik, B. A.; Kjeldsen, F.; Polfer, N. C.; Zubarev, R. A. Eur. J. Mass Spectrom. 2002, 8, 461.