Isolation and primary culture of rat hepatocytes

Hepatobiliary and Pancreatic Diseases International

Volumn 1, Issue 1, 2002, Pages 77-79

  Liu, Xiao Li ^a   Li, Lan Juan ^a   Chen, Zhi ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Artificial liver; Culture; Hepatocytes; Isolation; Primary

Indexed keywords

ALANINE AMINOTRANSFERASE; COLLAGENASE; FORMAZAN;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CELL ISOLATION; CELL STRUCTURE; CELL VIABILITY; CHEMICAL PROCEDURES; CONTROLLED STUDY; LIVER CELL CULTURE; NONHUMAN; PERFUSION; RAT; STATISTICAL ANALYSIS; SUPERNATANT; UREA CYCLE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELL SURVIVAL; COLLAGENASES; HEPATOCYTES; LIVER, ARTIFICIAL; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0038544620     PISSN: 14993872     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Li LJ, Huang JR, Chen YM, et al. Artificial liver support system in the treatment of severe hepatitis. Chin J Hepatol 1999; 17: 228-230.
2. Seglen PO. Preparation of isolated rat liver cells. Methods Cell Biol 1976; 13: 29-63.
3. Puviani AC, Ottolenghi C, Tassinari B, et al. An update on high-yield hepatocyte isolation methods and on the potential clinical use of isolated liver cells. Comp Biochem Physiol 1998; 121: 99-109.
4. Berthiaume F, Moghe PV, Toner M, et al. Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: hepatocytes cultured in a sandwich configuration. FASEB 1996; 10: 1471-1484.
5. Cable EE, Isom HC. Exposure of primary rat hepatocytes in long-term DMSO culture to selected transition metals induces hepatocytes proliferation and formation of duct-like structures. Hepatology 1997; 26: 1444-1457.