A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding protein GW182

Hybridoma and Hybridomics

Volumn 22, Issue 2, 2003, Pages 79-86

  Eystathioy, Theophany ^a   Chan, Edward K L ^b   Mahler, Michael ^c   Luft, Leeanne M ^a   Fritzler, Mark L ^a   Fritzler, Marvin J ^a,d  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF FLORIDA   (United States)

^c UNIVERSITY OF HEIDELBERG   (Germany)

^d UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; FORMALDEHYDE; GLYCINE; MONOCLONAL ANTIBODY; PARAFFIN; PROTEIN GW182; RNA BINDING PROTEIN; TRYPTOPHAN; UNCLASSIFIED DRUG;

ANTIGEN BINDING; ANTIGEN RETRIEVAL; ARTICLE; CELL CULTURE; CYTOPLASM; EPITOPE MAPPING; GW BODY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN MOTIF; RNA PROCESSING; TISSUE CULTURE; WESTERN BLOTTING;

EID: 0038512315     PISSN: 15368599     EISSN: None     Source Type: Journal    
DOI: 10.1089/153685903321947996     Document Type: Article

References (27)

1. Tan EM: Autoantibodies in pathology and cell biology. Cell 1991;67:841-842.
2. Lerner MR and Steitz JA: Snurps and scyrps. Cell 1981;25:298-300.
3. Craft J: Antibodies to snRNPs in systemic lupus erythematosus. Rheum Dis Clin N A 1992;18:311-335.
4. Luhrmann R: Functions of U-snRNPs. Mol Biol Rep 1990;14:183-192.
5. Eystathioy T, Chan EKL, Tenenbaum SA, Keene JD, Griffith KJ, and Fritzler MJ: A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. Mol Biol Cell 2002;13:1338-1351.
6. Tenenbaum SA, Carson CC, Lager PJ, and Keene JD: Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays. Proc Natl Acad Sci USA 2002;97:14085-14090.
7. Tenenbaum SA, Lager PJ, Carson CC and Keene JD: Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays. Methods 2002;26:191-198.
8. Keene JD: Why is Hu where? Shuttling of early-response-gene messenger RNA subsets. Proc Natl Acad Sci USA 1999;96:14085-14090.
9. Keene JD: Ribonucleoprotein infrastructure regulating the flow of genetic information between the genome and proteome. Proc Natl Acad Sci USA 2001;98:7018-7024.
10. Brennan CM and Steitz JA: HuR and mRNA stability. Cell Mol Life Sci 2001;58:266-277.
11. Paushkin S, Gubitz AK, Massenet S, and Dreyfuss G: The SMN complex, an assemblyosome of ribonucleoproteins. Cull Opin Cell Biol 2002;14:305-312.
12. Keene JD and Tenenbaum SA: Eukaryotic mRNPs may represent posttranscriptional operons. Mol Cell 2002;9:1161-1167.
13. Fritzler MJ: Immunofluorescent antinuclear antibody test. In: Manual of Clinical Laboratory Immunology, Rose NR, de Macario EC, Folds JD, Lane CL, and Nakamura RM (Eds), ASM Press, Washington, DC., 1997, 920-927.
14. Fritzler MJ, Lung C-C, Hamel JC, Griffith K and Chan EKL: Molecular characterization of golgin-245: A novel Golgi complex protein containing a granin signature. J Biol Chem 1995;270:31262-31268.
15. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680.
16. Frank R and Overwin H: SPOT synthesis. Epitope analysis with arrays of synthetic peptides prepared on cellulose membranes. Methods Mol Biol 1996;66:149-169.
17. Mahler M, Mierau R, and Blüthner M: Fine-specificity of the anti-CENP-A B-cell autoimmune response. J Mol Med 2000;78:460-467.
18. Frank R: The SPOT synthesis technique. Synthetic peptide arrays on membrane supports - principles and applications. J Immunol Methods 2002;267:13-26.
19. Iannone MA, Consler TG, Pearce KH, Stimmel JB, Parks DJ, and Gray JG: Multiplexed molecular interactions of nuclear receptors using fluorescent micorspheres. Cytometry 2001;44:326-337.
20. Bellisario R, Colinas RJ, and Pass KA: Simultaneous measurement of antibodies to three HIV-1 antigens in newborn dried blood-spot specimens using a multiplexed microsphere-based immunoassay. Early Hum Dev 2001;64:21-25.
21. Willman JH, Hill HR, Martins TB, Jankowski TD, Ashwood ER, and Litwin CM: Multiplex analysis of heterophil antibodies in patients with intermediate HIV immunoassay results. Am J Clin Pathol 2001;115:764-769.
22. Vignali DA: Multiplexed particle-based flow cytometric assays. J Immunol Meth 2000;21:243-255.
23. Vasudevan S, Peltz SW, and Wilusz CJ: Non-stop decay - a new mRNA surveillance pathway. Bioessays 2002;24:785-788.
24. Moore MJ: No end to nonsense. Science 2002;298:370-371.
25. Van Dijk E, Cougot N, Meyer S, Babajko S, Wahle E, Séraphin B: Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures. EMBO J 2002;21:6915-6924.
26. Ingelfinger D, Arndt-Jovin DJ, Luhrmann R, and Achsel T: The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci. RNA 2002;8:1489-1501.
27. Mazroui R, Huot ME, Tremblay S, Filion C, Labelle Y, Khandjian EW: Trapping of messenger RNA by Fragile X Mental Retardation protein into cytoplasmic granules induces translation repression. Hum Mol Genet 2002;11:3007-3017.