A whole blood flow cytometric determination of platelet activation by unfractionated and low molecular weight heparin in vitro

Thrombosis Research

Volumn 108, Issue 5-6, 2002, Pages 291-296

  Klein, Bernd ^a   Faridi, Andreé ^a   Von Tempelhoff, G F ^b   Heilmann, Lothar ^b   Mittermayer, Christian ^a   Rath, Werner ^a  

^a UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

^b NONE

Author keywords

Flow cytometry; Heparin; Low molecular weight heparin; Platelets; Thrombocytopenia; Whole blood

Indexed keywords

ADENOSINE DIPHOSPHATE; DALTEPARIN; FIBRINOGEN; FIBRINOGEN RECEPTOR; GLYCOPROTEIN; GLYCOPROTEIN GP 53; HEPARIN; LOW MOLECULAR WEIGHT HEPARIN; PADGEM PROTEIN; PROTEIN ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY LABELING; ARTICLE; BLOOD ANALYSIS; CELL SURFACE; CONTROLLED STUDY; CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; IN VITRO STUDY; NORMAL HUMAN; PROTEIN BINDING; THROMBOCYTE ACTIVATION; ADULT; BLOOD; DRUG EFFECT; FLOW CYTOMETRY;

ADENOSINE DIPHOSPHATE; ADULT; BLOOD; FIBRINOGEN; FLOW CYTOMETRY; HEPARIN; HEPARIN, LOW-MOLECULAR-WEIGHT; HUMANS; P-SELECTIN; PLATELET ACTIVATION;

EID: 0038506232     PISSN: 00493848     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0049-3848(03)00094-X     Document Type: Article

References (28)

1. Komarnicki M., Kazmierczak M., Haertle D. Standard heparin and low molecular weight heparin effect on spontaneous platelet aggregation measured in whole blood and in platelet-rich plasma. Acta Haematol. Pol. 24:1993;263-265.
2. Eika C. Platelet refractory state induced by heparin. Scand. J. Haematol. 9:1972;665-672.
3. Salzman E.W., Rosenberg R.D., Smith M.H., Lindon J.N., Favreau L. Effect of heparin and heparin fractions on platelet aggregation. J. Clin. Invest. 65:1980;64-73.
4. Chong B.H., Ismail F. The mechanism of heparin-induced platelet aggregation. Eur. J. Haematol. 43:1989;245-251.
5. Chen J.H., Karlberg K.E., Sylven C. Heparin and low molecular weight heparin but not hirudin stimulate platelet aggregation in whole blood from acetylsalicylic acid treated healthy volunteers. Thromb. Res. 63:1991;319-329.
6. Heinrich D., Gorg T., Schulz M. Effects of unfractionated and fractionated heparin on platelet function. Haemostasis. 18(Suppl. 3):1988;48-54.
7. Ellison N., Edmunds L.H. Jr., Colman R.W. Platelet aggregation following heparin and protamine administration. Anesthesiology. 48:1978;65-68.
8. Melandri G., Semprini F., Cervi V.et al. Comparison of efficacy of low molecular weight heparin (parnaparin) with that of unfractionated heparin in the presence of activated platelets in healthy subjects. Am. J. Cardiol. 72:1993;450-454.
9. Thomson C., Forbes C.D., Prentice C.R. The potentiation of platelet aggregation and adhesion by heparin in vitro and in vivo. Clin. Sci. Mol. Med. 45:1973;485-494.
10. Dunn F.W., Soria C., Thomaidis A.et al. Interactions of platelets with standard heparin and low molecular weight fractions. Nouv. Rev. Fr. Hematol. 26:1984;249-253.
11. Mikhailidis D.P., Barradas M.A., Mikhailidis A.M., Magnani H., Dandona P. Comparison of the effect of a conventional heparin and a low molecular weight heparinoid on platelet function. Br. J. Clin. Pharmacol. 17:1984;43-48.
12. Kawano K., Ikeda Y., Handa M.et al. Enhancing effect by heparin on shear-induced platelet aggregation. Semin. Thromb. Hemost. 16:1990;60-65. [Suppl].
13. Mastacchi R., Stanzani L., Barbanti M., Montecchi L., Bianchini P. Interaction of heparin and heparin fractions with human platelets. Thromb. Res. 28:1982;275-280.
14. Brace L.D., Fareed J. Heparin-induced platelet aggregation: dose/ response relationships for a low molecular weight heparin derivative (PK 10169) and its subfractions. Thromb. Res. 42:1986;769-782.
15. Westwick J., Scully M.F., Poll C., Kakkar V.V. Comparison of the effects of low molecular weight heparin and unfractionated heparin on activation of human platelets in vitro. Thromb. Res. 42:1986;435-447.
16. Mastacchi R., Barbanti M., Bianchini P., Osima B. Interaction of a new low molecular weight heparin (OP/LMWH) with human platelets. Agents Actions. 17:1986;512-514.
17. Cella G., Scattolo N., Luzzatto G., Girolami A. Effects of low-molecular-weight heparin on platelets as compared with commercial heparin. Res. Exp. Med. Berl. 184:1984;227-229.
18. Zwierzina W.D., Kunz F. A method of testing platelet aggregation in native whole blood. Thromb. Res. 38:1985;91-100.
19. John L.C., Rees G.M., Kovacs I.B. Effect of heparin on in vitro platelet reactivity in cardiac surgical patients - a comparative assessment by whole blood platelet aggregometry and haemostatometry. Thromb. Res. 66:1992;649-656.
20. Shattil S.J., Cunningham M., Hoxie J.A. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood. 70:1987;307-315.
21. Niiya K., Hodson E., Bader R.et al. Increased surface expression of the membrane glycoprotein IIb/IIIa complex induced by platelet activation. Relationship to the binding of fibrinogen and platelet aggregation. Blood. 70:1987;475-483.
22. Janes S.L., Wilson D.J., Chronos N., Goodall A.H. Evaluation of whole blood flow cytometric detection of platelet bound fibrinogen on normal subjects and patients with activated platelets. Thromb. Haemost. 70:1993;659-666.
23. Warkentin T.E., Powling M.J., Hardisty R.M. Measurement of fibrinogen binding to platelets in whole blood by flow cytometry: a micromethod for the detection of platelet activation. Br. J. Haematol. 76:1990;387-394.
24. Vermylen J. Effect of heparin and low molecular weight heparins on platelets. Semin. Thromb. Hemost. 19(Suppl. 1):1993;20-21.
25. Warkentin T.E., Kelton J.G. Heparin and platelets. Hematol. Oncol. Clin. North Am. 4:1990;243-264.
26. Chong B.H. Heparin-induced thrombocytopenia. Aust. N. Z. J. Med. 22:1992;145-152.
27. Krupinski K., Basic Micic M., Lindhoff E., Breddin H.K. Inhibition of coagulation and platelet adhesion to extracellular matrix by unfractionated heparin and a low molecular weight heparin. Blut. 61:1990;289-294.
28. Ljungberg B., Beving H., Egberg N., Johnsson H., Vesterqvist O. Immediate effects of heparin and LMW heparin on some platelet and endothelial derived factors. Thromb. Res. 51:1988;209-217.