Analysis of saliva for antibodies to the LPS of Escherichia coli O157 in patients with serum antibodies to E. coli O157 LPS

Journal of Medical Microbiology

Volumn 52, Issue 7, 2003, Pages 569-572

  Chart, Henrik ^a   Perry, Neil T ^a   Willshaw, Geraldine A ^a   Cheasty, Thomas ^a  

^a Lab of Enteric Pathogens Ctrl P   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE;

ADULT; ANTIBODY RESPONSE; ANTIGEN BINDING; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; FEMALE; HUMAN; IMMUNOASSAY; MALE; PRIORITY JOURNAL; SALIVA ANALYSIS; SALIVA LEVEL; SENSITIVITY AND SPECIFICITY;

ADOLESCENT; ADULT; ANTIBODIES, BACTERIAL; CHILD; CHILD, PRESCHOOL; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI O157; FEMALE; HUMANS; LIPOPOLYSACCHARIDES; MALE; MIDDLE AGED; SALIVA;

EID: 0038441811     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.05126-0     Document Type: Article

References (14)

1. Chart, H. (1994). Bacterial fractionation and membrane protein characterization. In Methods in Practical Laboratory Bacteriology, pp. 1-20. Edited by H. Chart. Boca Raton, Ann Arbor, London, Tokyo: CRC Press.
2. Chart, H. & Jenkins, C. (1998). Salivary antibodies to lipopolysaccharide antigens of Escherichia coli O157. Lancet 352, 371.
3. Chart, H. & Jenkins, C. (1999). The serodiagnosis of infections caused by Verocytotoxin-producing Escherichia coli. J Appl Microbiol 86, 731-740.
4. Chart, H., Scotland, S. M. & Rowe, B. (1989a). Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol 27, 285-290.
5. Chart, H., Scotland, S. M., Smith, H. R. & Rowe, B. (1989b). Antibodies to Escherichia coli O157 in patients with haemorrhagic colitis and haemolytic uraemic syndrome. J Clin Pathol 42, 973-976.
6. Chart, H., Perry, N. T., Cheasty, T. & Wright, P. A. (2002). The kinetics of antibody production to antigens of Escherichia coli O157 in a pregnant woman with haemolytic uraemic syndrome. J Med Microbiol 51, 522-525.
7. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
8. Ludwig, K., Grabhorn, E., Bitzan, M., Bobrowski, C., Kemper, M. J., Sobottka, I., Laufs, R., Karch, H. & Muller-Wiefel, D. E. (2002). Saliva IgM and IgA are a sensitive indicator of the humoral immune response to Escherichia coli O157 lipopolysaccharide in children with enteropathic hemolytic uremic syndrome. Pediatr Res 52, 307-313.
9. Simmons, N. A. (1997). Global views on Escherichia coli O157:H7 and other Verocytotoxic E. coli spp.: UK views. J Food Prot 60, 1463-1465.
10. Thomas, A., Cheasty, T., Frost, J. A., Chart, H., Smith, H. R. & Rowe, B. (1996). Verocytotoxin-producing Escherichia coli O157, particularly serogroup O157, associated with human infections in England and Wales: 1992-4. Epidemiol Infect 117, 1-10.
11. Tsai, C.-M. & Frasch, C. E. (1982). A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Anal Biochem 119, 115-119.
12. Westphal, O. & Jann, K. (1965). Bacterial lipopolysaccharides. Extraction with phenol-water and further application of the procedure. Methods Carbohydr Chem 5, 83-91.
13. Willshaw, G. A., Cheasty, T., Smith, H. R., O'Brien, S. J. & Adak, G. K. (2001). Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998. J Med Microbiol 50, 135-142.
14. Wray, W., Boulikas, T., Wray, V. P. & Hancock, R. (1981). Silver staining of proteins in polyacrylamide gels. Anal Biochem 118, 197-203.