The mitochondrial uncoupler dicumarol disrupts the MTT assay

Biochemical Pharmacology

Volumn 66, Issue 2, 2003, Pages 281-287

  Collier, Abby C ^a   Pritsos, Chris A ^a  

^a UNIVERSITY OF NEVADA   (United States)

Author keywords

Cytotoxicity; Dicumarol; Mitochondria; MTT assay; NQO1; Oxidative stress

Indexed keywords

3 (4,4 DIMETHYLTHIAZOL 2 YL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BROMINE DERIVATIVE; DICOUMAROL; OXYGEN; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BREAST TUMOR; CATTLE; CELL SURVIVAL; CELL VIABILITY; CHO CELL; CLONOGENIC ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; INCUBATION TIME; MITOCHONDRION; MOUSE; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; SERUM; TUMOR CELL LINE;

EID: 0038433339     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(03)00240-5     Document Type: Article

References (17)

1. Slater T., Sawyer B., Strauli U. Studies on succinate-tetrazolium reductase system. III. Points of coupling of four different tetrazolium salts. Biochim. Biophys. Acta. 77:1963;383-393.
2. Berridge M., Tan A. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 303(2):1993;474-482.
3. Gonzalez R., Tarloff J. Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity. Toxicol. In Vitro. 15(3):2001;257-259.
4. Bernas T., Dobrucki J. Mitochondrial and nonmitochondrial reduction of MTT: interaction of MTT with TMRE, JC-1, and NAO mitochondrial fluorescent probes. Cytometry. 47(4):2002;236-242.
5. Prochaska H., Santamaria A. Direct measurement of NAD(P)H:quinone reductase from cells cultured in microtiter wells: a screening assay for anticarcinogenic enzyme inducers. Anal. Biochem. 169(2):1988;328-336.
6. Goodwin C., Holt S., Riley P., Downes S., Marshall N. Growth hormone-responsive DT-diaphorase-mediated bioreduction of tetrazolium salts. Biochem. Biophys. Res. Commun. 226:1997;935-941.
7. Long D., Jaiswal A. NRH:quinone oxidoreductase 2. Chem. Biol. Interact. 129:2000;99-112.
8. Lind C., Cadenas E., Hochstein P., Ernster L. DT-diaphorase: purification, properties, and function. Methods Enzymol. 186:1990;287-301.
9. Segura-Aguilar J., Barreiro V., Lind C. Dicoumarol-sensitive glucuronidation of benzo(a)pyrene metabolites in rat liver microsomes. Arch. Biochem. Biophys. 251(1):1986;266-275.
10. Gustafson D., Pritsos C. Enhancement of xanthine dehydrogenase mediated mitomycin C metabolism by dicumarol. Cancer Res. 52(24):1992;6936-6939.
11. Schulze-Osthoff K., Bakker A., Vanhaesebroeck B., Beyaert R., Jacob W., Fiers W. Cytotoxic activity of tumor necrosis factor is mediated by early damage of mitochondrial functions. J. Biol. Chem. 267(8):1992;5317-5323.
12. Rockwell S.C., Kallman R.F., Fajardo L.F. Characteristics of a serially transplanted mouse mammary tumour and its tissue-culture-adapted derivative. J. Natl. Cancer Inst. 49(3):1972;735-749.
13. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65(1/2):1983;55-63.
14. Teicher B.A., Lazo J.S., Sartorelli A.C. Classification of antineoplastic agents by their selective toxicities towards oxygenated and hypoxic tumor cells. Cancer Res. 41:1981;73-81.
15. Rosenkranz A.R., Schmaldienst S., Stuhlmeier K.M., Chen W., Knapp W., Zlabinger G.J. A microplate assay for the detection of oxidative products using 2′,7′-dichlorofluorescein-diacetate. J. Immunol. Methods. 156:1992;39-45.
16. Briggs L., Pritsos C. Relative contributions of mouse liver subcellular fractions to the bioactivation of mitomycin C at various pH levels. Biochem. Pharmacol. 58(10):1999;1609-1614.
17. Belo R., Gomez-Diaz C., Navarro F., Alcain F., Villalba J. Expression of NAD(P)H:quinone oxido-reductase 1 in HeLa cells. J. Biol. Chem. 276(48):2001;44379-44384.