Qualitative determination of false-positive effects in the acetylcholinesterase assay using thin layer chromatography

Phytochemical Analysis

Volumn 14, Issue 3, 2003, Pages 127-131

  Rhee, In K ^a   van Rijn, Richard M ^a   Verpoorte, Robert ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Acetylcholinesterase inhibition; Ellman's method; False positive effects; Nerine bowdenii; TLC assay

Indexed keywords

ENZYME ACTIVITY; ENZYME INHIBITION; ETHANOLAMINES; TRIETHANOLAMINE;

ACETYLCHOLINESTERASE; ACETYLCHOLINESTERASE INHIBITION; CHEMICAL INHIBITION; ELLMAN METHOD; FALSE POSITIVE; FALSE-POSITIVE EFFECT; NERINE BOWDENII; THIN-LAYER CHROMATOGRAPHY; THIOCHOLINE; TLC ASSAY;

THIN LAYER CHROMATOGRAPHY;

4 DIMETHYLAMINOBENZALDEHYDE; 4 HYDROXYBENZALDEHYDE; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); ACETYLCHOLINESTERASE; ALDEHYDE DERIVATIVE; AMINE; ANISALDEHYDE; BENZALDEHYDE; CHOLINESTERASE INHIBITOR; CINNAMALDEHYDE; DIETHYLAMINE; GALANTAMINE; HEXYLAMINE; NERINE EXTRACT; PLANT EXTRACT; THIOCHOLINE; TOLUENE; TRIETHANOLAMINE; TRIETHYLAMINE; TRYPTAMINE; TYRAMINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; EXTRACTION; FRACTIONATION; ISOLATION PROCEDURE; MEDICINAL PLANT; NERINE; NONHUMAN; QUALITATIVE ANALYSIS; THIN LAYER CHROMATOGRAPHY;

NERINE BOWDENII;

EID: 0038413983     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/pca.675     Document Type: Article

References (5)

1. Dawson RMC, Elliott DC, Elliott WH and Jones KM. 1986. Data for Biochemical Research, 3rd edn. Clarendon Press: Oxford; 456-458.
2. Ellman GL, Courtney D, Andies V and Featherstone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmac 7: 88-95.
3. Ingkaninan K, de Best CM, van der Heijden R, Hofte AJP, Karabatak B, Irth H, Tjaden UR, van der Greef J and Verpoorte R. 2000. HPLC with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products. J Chromatogr A 872: 61-73.
4. Perry EK. 1986. The cholinergic hypothesis - ten years on. Br Med Bull 42: 63-69.
5. Rhee IK, van de Meent M, Ingkaninan K and Verpoorte R. 2001. Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining. J Chromatogr A 915: 217-223.