Characterization of rat α(1,3)galactosyltransferase: Evidence for two independent genes encoding glycosyltransferases that synthesize Galα(1,3)Gal by two separate glycosylation pathways

Glycobiology

Volumn 13, Issue 5, 2003, Pages 327-337

  Taylor, Simon G ^a   McKenzie, Ian F C ^a   Sandrin, Mauro S ^a  

^a AUSTIN RESEARCH INSTITUTE   (Australia)

Author keywords

1,3galactosyltransferase; Gal (1,3)Gal; Glycolipid; Xenotransplantation

Indexed keywords

GALACTOSYLTRANSFERASE; GLYCOLIPID; GLYCOSYLTRANSFERASE; SYNTHETASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME SYNTHESIS; GENETIC TRANSFECTION; GLYCOSYLATION; LIPID ANALYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RAT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; DISACCHARIDES; GALACTOSYLTRANSFERASES; GLYCOLIPIDS; GLYCOPROTEINS; GLYCOSYLATION; GLYCOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; RATS; RATS, SPRAGUE-DAWLEY; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; RODENTIA; SUS SCROFA;

EID: 0038394491     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/cwg030     Document Type: Article

References (26)

1. Basu, M. and Basu, S. (1973) Enzymatic synthesis of a blood group B-related pentaglycosylceramide by an alpha-galactosyltransferase from rabbit bone marrow. J. Biol. Chem., 248, 1700-1706.
2. Blanken, W.M. and Van den Eijnden, D.H. (1985) Biosynthesis of terminal Gal alpha 1-3Gal beta 1-4GlcNAc-R oligosaccharide sequences on glycoconjugates. Purification and acceptor specificity of a UDP-Gal:N-acetyllactosaminide alpha 1-3-galactosyltransferase from calf thymus. J. Biol. Chem., 260, 12927-12934.
3. Breimer, M.E., Hansson, G.C., Karlsson, K.A., and Leffler, H. (1981) Glycosphingolipids and the differentiation of intestinal epithelium. Exp. Cell Res., 135, 1-13.
4. Coligan, J.E., Dunn, B.M., Speicher, D.W., and Wingfield, P.T. (1995) Current protocols in protein science. New York: Wiley.
5. Haslam, D.B. and Baenziger, J.U. (1996) Expression cloning of Forssman glycolipid synthetase: a novel member of the histo-blood group ABO gene family. Proc. Natl. Acad. Sci. USA, 93, 10697-10702.
6. Heffernan, M. and Dennis, J.W. (1991) Polyoma and hamster papovavirus large T antigen-mediated replication of expression shuttle vectors in Chinese hamster ovary cells. Nucleic Acids Res., 19, 85-92.
7. Joziasse, D.H., Shaper, J.H., Van den Eijnden, D.H., Van Tunen, A.J., and Shaper, N.L. (1989) Bovine alpha 1-3-galactosyltransferase: isolation and characterization of a cDNA clone. Identification of homologous sequences in human genomic DNA. J. Biol. Chem., 264, 14290-14297.
8. Joziasse, D.H., Shaper, N.L., Kim, D., Van den Eijnden, D.H., and Shaper, J.H. (1992) Murine alpha 1,3-galactosyltransferase. A single gene locus specifies four isoforms of the enzyme by alternative splicing. J. Biol. Chem., 267, 5534-5541.
9. Keusch, J.J., Manzella, S.M., Nyame, K.A., Cummings, R.D., and Baenziger, J.U. (2000a) Cloning of Gb3 synthase, the key enzyme in globo-series glycosphingolipid synthesis, predicts a family of alpha 1,4-glycosyltransferases conserved in plants, insects, and mammals. J. Biol. Chem., 275, 25315-25321.
10. Keusch, J.J., Manzella, S.M., Nyame, K.A., Cummings, R.D., and Baenziger, J.U. (2000b) Expression cloning of a new member of the ABO blood group glycosyltransferases, iGb3 synthase, that directs the synthesis of isoglobo-glycosphingolipids. J Biol, Chem., 275, 25308-25314.
11. Larsen, R.D., Rajan, V.P., Ruff, M.M., Kukowska-Latallo, J., Cummings, R.D., and Lowe, J.B. (1989) Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer. Proc. Natl. Acad. Sci. USA, 86, 8227-8231.
12. Nelson, P.W., Helling, T.S., Shield, C.F., Beck, M., and Bryan, C.F. (1992) Current experience with renal transplantation across the ABO barrier. Am. J. Surg., 164, 541-544; discussion 544-545.
13. Ogiso, M., Nishiyama, I., Saito, N., Okinaga, T., Hoshi, M., and Komoto, M. (1995) Localization of neutral and acidic glycosphingolipids in rat lens. Glycobiology, 5, 187-194.
14. Ogiso, M., Ohta, M., Irie, A., Hoshi, M., and Komoto, M. (1995) Characterization of neutral glycosphingolipids in rat lens. Exp. Eye Res., 60, 193-198.
15. Rosales Fritz, V.M., Daniotti, J.L., and Maccioni, H.J. (1997) Chinese hamster ovary cells lacking GM1 and GD1a synthesize gangliosides upon transfection with human GM2 synthase. Biochim. Biophys. Acta, 1354, 153-158.
16. Sambrook, J.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
17. Sandrin, M.S. and McKenzie, I.F. (1999) Recent advances in xenotransplantation. Curr. Opin. Immunol., 11, 527-531.
18. Sandrin, M.S., Vaughan, H.A., Dabkowski, P.L., and McKenzie, I.F. (1993) Anti-pig IgM antibodies in human serum react predominantly with Gal(alpha 1-3)Gal epitopes. Proc. Natl. Acad. Sci. USA, 90, 11391-11395.
19. Sandrin, M.S., Dabkowski, P.L., Henning, M.M., Mouhtouris, E., and McKenzie, I.F.C. (1994a) Characterization of cDNA clones for porcine alpha(1,3)galactosyl transferase: the enzyme generating the Galalpha(1,3)Gal epitope. Xenotransplantation, 1, 81-88.
20. Sandrin, M.S., Vaughan, H.A., and McKenzie, I.F.C. (1994b) Identification of Gal(alpha1,3)Gal as the major epitope for pig-to-human vascularised xenografts. Transplant. Rev., 8, 134-149.
21. Sandrin, M.S., Milland, J.A., Taylor, S.G., Lazarus, B.D. Dodson, H.C., Xing, P.-X., and McKenzie, I.F.C. (2001) A second galactosyltransferase which synthesizes the major xenoepitope Gala(1,3)Gal. Xenotransplantation, 8(suppl 1), 27.
22. Sandrin, M.S., Milland, J., Dodson, H.C., Lazarus, B.D., Taylor, S.G., and McKenzie, I.F.C. (2002) Galα(1,3)Gal: description of a second gene and transgenic approaches to reduce Gal. Transplantation, 74, 31.
23. Schnaar, R.L. (1994) Isolation of glycosphingolipids. Methods Enzymol., 230, 348-370.
24. Slomiany, B.L., Slomiany, A., and Horowitz, M.I. (1974) Characterization of blood-group-H-active ceramide tetrasaccharide from hog-stomach mucosa. Eur. J. Biochem., 43, 161-165.
25. Vanhove, B., Goret, F., Soulillou, J.P., and Pourcel, C. (1997) Porcine alpha1,3-galactosyltransferase: tissue-specific and regulated expression of splicing isoforms. Biochim. Biophys. Acta, 1356, 1-11.
26. Williams, G.M., Hume, D.M., Hudson, R.P. Jr., Morris, P.J., Kano, K., and Milgrom, F. (1968) "Hyperacute" renal-homograft rejection in man. N. Engl. J. Med., 279, 611-618.