A simple method of rat renal brush border membrane preparation using polyethylene glycol precipitation

International Journal of Biochemistry and Cell Biology

Volumn 35, Issue 8, 2003, Pages 1248-1255

  Basivireddy, Jayasree ^a   Balasubramanian, K A ^a  

^a CHRISTIAN MEDICAL COLLEGE   (India)

Author keywords

Brush border membrane; Lipid composition; Polyethylene glycol; Renal

Indexed keywords

ALKALINE PHOSPHATASE; AMINOPEPTIDASE; CHOLESTEROL; FUCOSE; GAMMA GLUTAMYLTRANSFERASE; GLUCOSE; HEXOSE; MACROGOL; MEMBRANE LIPID; PHOSPHOLIPID; SPHINGOMYELIN;

ANIMAL TISSUE; ARTICLE; BASOLATERAL MEMBRANE; BRUSH BORDER; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; ELECTRON MICROSCOPY; GLUCOSE TRANSPORT; KIDNEY HOMOGENATE; KIDNEY MICROSOME; KIDNEY TUBULE EPITHELIUM; LIPID ANALYSIS; LIPID COMPOSITION; LYSOSOME; MEMBRANE VESICLE; NONHUMAN; PEROXISOME; RAT;

EID: 0038363844     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(02)00345-X     Document Type: Article

References (35)

1. 2+ precipitation. Biochemistry and Biophysics Acta. 856:1986;610-614.
2. Bartlett G.R. Phosphorous assay in column chromatography. Journal of Biological Chemistry. 234:1959;466-468.
3. Bjorkman D.J., Brigham E.J. Differences in composition and fluidity of intestinal microvillus membrane vesicles prepared by different methods. Biochemical and Biophysical Research Communications. 170:1990;433-440.
4. Bjorkman D.J., Hagen C.H., Trier J.S. Structural features of absorptive cell and microvillus membrane preparations from rat small intestine. Gastroenterology. 91:1986;1401-1414.
5. Bliigh E.G., Dyer W.J. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology. 37:1959;911-917.
6. Booth A.G., Kenny J. A rapid method for the preparation of microvilli from rabbit kidney. Biochemical Journal. 142:1974;575-581.
7. Brasitus T.A., Keresztes R.S. Protein-lipid interactions in antipodal plasma membranes of rat colonocytes. Biochemistry and Biophysics Acta. 773:1984;290-300.
8. Djurdjic V., Mandic L. A spectrophotometric method for simultaneous determination of protein-bound hexoses and fucose with a mixture of L-cysteine and phenol. Analytical Biochemistry. 188:1990;222-227.
9. Dorai D.T., Bachhawat B.K. Purification and properties of brain alkaline phosphatase. Journal of Neurochemistry. 29:1977;503-512.
10. Ganapathy V., Mendicino J.F., Leibach F.H. Transport of glycyl-L-proline into intestinal and renal brush border vesicles from rabbit. Journal of Biological Chemistry. 256:1981;118-124.
11. Gianetto R., De Duve C. Comparative study of binding if acid phosphatase beta-glucoronidase and catheppsin by rat liver particles. Biochemical Journal. 59:1955;433-438.
12. Glossmann H., Neville M. Jr. Glycoproteins of cell surfaces. Journal of Biological Chemistry. 246:1971;6339-6346.
13. Grimellec C., Giocondi M.C., Carriere B., Cardinal J. Fluidity of brush border and basolateral membranes from human kidney cortex. American Journal of Physiology. 245:1983;F227-F231.
14. Habeeb H.W.S. Reaction of protein sulfhydryl groups with Ellman's reagent. Methods in Enzymology. 25:1972;457-464.
15. Hubscher G., West G.R., Brindley D.N. Studies on the fractionation of mucosal homogenates from the small intestine. Biochemical Journal. 97:1965;629-642.
16. 2+ precipitation. Comparative Biochemistry and Physiology. 112B:1995;65-69.
17. Indrani N., Hill P.G. Partial purification and some properties of gamma-glutamyl transpeptidase from human bile. Biochemistry and Biophysics Acta. 483:1977;57-62.
18. Kessler M., Acuto O., Storelli C., Murer H., Muller M., Semenza G. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems. Biochemistry and Biophysics Acta. 506:1978;136-154.
19. Kroes J., Ostwald R. Erythrocyte membranes - effect of increased cholesterol content on permeability. Biochemistry and Biophysics Acta. 249:1971;647-650.
20. Kumar R., Prasad R. Functional characterization of purified zinc transporter from renal brush border membrane of rat. Biochemistry and Biophysics Acta. 509:2000;429-439.
21. + dependent cotransport across renal brush border membranes. American Journal of Physiology, 257, F126-F136.
22. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 193:1970;265-275.
23. Molitoris B.A., Simon F.R. Renal cortical brush border and basolateral membranes: Cholesterol and phospholipid composition and relative turn over. Journal of Membrane Biology. 83:1985;207-215.
24. 2+)-dependent adenosine triphosphatase. Biochemistry and Biophysics Acta. 776:1984;237-246.
25. Pfleiderer G. Particle bound amino peptidase from pig kidney. Methods in Enzymology. 19:1970;514-521.
26. Prabhu R., Balasubramanian K.A. A novel method of preparation of small intestinal brush border membrane vesicles by polyethylene glycol precipitation. Analytical Biochemistry. 289:2001;157-161.
27. Proulx P. Structure-function relationships in intestinal brush border membranes. Biochemistry and Biophysics Acta. 1071:1991;255-271.
28. +)-stimulated ATPase activity in rat intestinal mucosa. Biochemistry and Biophysics Acta. 173:1969;456-468.
29. Quirk S.J., Robinson G.B. Isolation and characterisation of rabbit renal brush borders. Biochemical Journal. 128:1972;1319-1328.
30. Serifer A., Gerstenfeld S. Photometric determination of sialic acid I serum and cerebrospinal fluid with thiobarbuteric acid method. Clinical Chemistry Acta. 7:1962;467-475.
31. Smedt D.H., Kinne R. Temperature dependence of solute transport and enzyme activities in hog renal brush border membrane vesicles. Biochemistry and Biophysics Acta. 648:1981;247-253.
32. Synder F., Stephen N. A simplified spectrophotometric determination of ester groups in lipids. Biochemistry and Biophysics Acta. 34:1959;244-245.
33. Thuneberg L., Rostgard J. Isolation of brush border fragments from homogenates of rat and rabbit kidney cortex. Experimental Cell Research. 51:1968;123-140.
34. Tiruppathi C., Miyamoto Y., Ganapathi V., Leibach F.H. Fatty acid-induced alterations in transport systems of the small intestinal brush-border membrane. Biology and Pharmacology. 37:1988;1399-1405.
35. Zaltkis A., Zak B., Boyle A.J. A new method for the direct determination of serum cholesterol. Journal of Laboratory and Clinical Medicine. 41:1953;486-492.