A continuous anaerobic fluorimetric assay for ferrochelatase by monitoring porphyrin disappearance

Analytical Biochemistry

Volumn 318, Issue 1, 2003, Pages 18-24

  Shi, Zhen ^a   Ferreira, Gloria C ^a,b  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b H LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE   (United States)

Author keywords

Anaerobic; Continuous assay; Ferrochelatase; Ferrous iron; Fluorescence; Initial rate; Protoporphyrin; Steady state kinetics

Indexed keywords

FLUORESCENCE; KINETICS;

ANAEROBIC; CONTINUOUS ASSAY; FERROCHELATASE; FERROUS IRON; INITIAL RATE; PROTOPORPHYRINS; STEADY-STATE KINETICS;

PORPHYRINS;

FERROCHELATASE; FERROUS ION; HEME; PROTOPORPHYRIN;

ACCURACY; ANAEROBIC CAPACITY; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; NONHUMAN; PRIORITY JOURNAL; SPECTROFLUOROMETRY; STEADY STATE;

EID: 0038362553     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00175-1     Document Type: Article

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