Synthesis of a radioactive thiol reagent, 1-S-[3H]carboxymethyl-dithiothreitol: Identification of the phosphorylation sites by N-terminal peptide sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Analytical Biochemistry

Volumn 319, Issue 1, 2003, Pages 143-158

  Salih, Erdjan ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

MALDI TOF MS; N terminal peptide sequencing; Phosphoproteins; Phosphorylation sites; Radioactive thiol reagent

Indexed keywords

DESORPTION; ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDES; PHOSPHORYLATION; RADIOACTIVITY; RADIOGRAPHY; REAGENTS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

MALDI TOF MS; PEPTIDE SEQUENCING; PHOSPHOPROTEINS; PHOSPHORYLATION SITES; THIOL REAGENTS;

MASS SPECTROMETRY;

ASPARAGINYLSERYLVALYLSERYLGLUTAMINYLGLUTAMINYLGLYCYLARGINYLGLUTAMINYLASP ART YLSERYLVALINE; CARBOXYMETHYLDITHIOTHREITOL H 3; CASEIN; IODOACETIC ACID; PHOSPHOPEPTIDE; PHOSPHOSERINE; PROTEIN SUBUNIT; RADIOISOTOPE; THIOL REAGENT; TRYPSIN; UNCLASSIFIED DRUG;

ACCURACY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; AUTORADIOGRAPHY; DERIVATIZATION; ISOTOPE LABELING; LABORATORY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PEPTIDE ANALYSIS; PEPTIDE MAPPING; PEPTIDE SYNTHESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RADIOACTIVITY; REACTION ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; SEQUENCE ANALYSIS; SYNTHESIS;

EID: 0038353301     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00254-9     Document Type: Article

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