A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater

Journal of Bioscience and Bioengineering

Volumn 95, Issue 6, 2003, Pages 637-640

  Morohoshi, Tomohiro ^a   Yamashita, Tomohiro ^a   Kato, Junichi ^a   Ikeda, Tsukasa ^a   Takiguchi, Noboru ^a   Ohtake, Hisao ^a   Kuroda, Akio ^a,b  

^a HIROSHIMA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Acinetobacter; Activated sludge; Pho regulon; Phosphate removal; Polyphosphate; Pseudomonas putida

Indexed keywords

BACTERIA; BIOLOGICAL WATER TREATMENT; PHOSPHATE MINERALS;

MUTANTS;

WATER POLLUTION CONTROL;

5 BROMO 4 CHLORO 3 INDOLYL PHOSPHATE; METHYLNITRONITROSOGUANIDINE; PHOSPHATE; POLYPHOSPHATE; UNCLASSIFIED DRUG;

ACTIVATED SLUDGE; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; ESCHERICHIA COLI; MUTAGENESIS; MUTATION; NUCLEOTIDE SEQUENCE; SCREENING; SOIL MICROFLORA; WASTE WATER; WATER ANALYSIS;

ACINETOBACTER; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0038344436     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(03)80177-9     Document Type: Article

References (13)

1. Hammond, A. L.: Phosphate replacements; problems with the washday miracles. Science, 172, 361-363 (1971).
2. Sedlak, R. I.: Phosphorus and nitrogen removal from municipal wastewater, 2nd ed. Lewis Publishers, Florida (1991).
3. Kato, J., Yamada, K., Muramatsu, A., Hardoyo, and Ohtake, H.: Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater. Appl. Environ. Microbiol., 59, 3744-3749 (1993).
4. Ohtake, H., Takahashi, Y., Tsuzuki, Y., and Toda, K.: Uptake and release of phosphate by a pure culture of Acinetobacter calcoaceticus. Water Res., 19, 1587-1594 (1985).
5. Abelson, P. H.: A potential phosphate crisis. Science, 283, 2015 (1999).
6. Kuroda, A., Takiguchi, N., Gotanda, T., Nomura, K., Kato, J., Ikeda, T., and Ohtake, H.: A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling. Biotechnol. Bioeng., 78, 333-338 (2002).
7. Arun, V., Mino, T., and Matsuo, T.: Biological mechanism of acetate uptake mediated by carbohydrate consumption in excess phosphorus removal systems. Water Res., 22, 565-570 (1988).
8. Kornberg, A.: Inorganic polyphosphate: toward making a forgotten polymer unforgettable. J. Bacteriol., 177, 491-496 (1995).
9. Morohoshi, T., Maruo, T., Shirai, Y., Kato, J., Ikeda, T., Takiguchi, N., Ohtake, H., and Kuroda, A.: Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp. strain PCC6803. Appl. Environ. Microbiol., 68, 4107-4110 (2002).
10. Neidhardt, F. C., Bloch, P. L., and Smith, D. F.: Culture medium for enterobacteria. J. Bacteriol., 119, 736-747 (1974).
11. Marchesi, J. R., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hiom, S. J., Dymock, D., and Wade, W. G.: Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl. Environ. Microbiol., 64, 795-799 (1998).
12. Ault-Riché, D., Fraley, C. D., Tzeng, C.-M., and Kornberg, A.: Novel assay reveals multiple pathways regulating stress-induced accumulations of inorganic polyphosphate in Escherichia coli. J. Bacteriol., 180, 1841-1847 (1998).
13. Steed, P. M. and Wanner, B. L.: Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J. Bacteriol., 175, 6797-6809 (1993).