The antimitotic effect of the neem terpenoid azadirachtin on cultured insect cells

Insect Biochemistry and Molecular Biology

Volumn 33, Issue 7, 2003, Pages 681-689

  Salehzadeh, A ^a   Akhkha, A ^a   Cushley, W ^a   Adams, R L P ^a   Kusel, J R ^a   Strang, R H C ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Antimitotic effect; Azadirachtin; Colchicine; Microtubules; Sf9 cells; Tubulin

Indexed keywords

AZADIRACHTA INDICA; INSECTA; MAMMALIA; MURINAE;

EID: 0038304894     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(03)00057-2     Document Type: Article

References (41)

1. Akudugu, J., Gade, G., Bohm, L., 2001. Cytotoxicity of azadirachtin A in human glioblastoma cell lines. Life Sci. 68, 1153-1160.
2. Ascher, K.R.S., Eliyahu, M., Nemny, N.E., Meisner, J., 1984. Neem NSKE as an inhibitor of growth and fecundity in S. littoralis. In: Schmuterer, H., Ascher, K.R.S. (Eds.), Proc. 2nd Int. Neem Conf., Rauischholhausen, Deutschland, Natural pesticides from the Neem Tree and other Tropical Plants, pp. 331-344.
3. Bai, R., Pei, X., Boyé, O., Getahun, Z., Grover, S., Bekisz, J., 1996. Identification of cysteine 354 of β-tubulin as part of the binding site for the A ring of colchicine. J. Biol. Chem. 271, 12639-12645.
4. Beitzen-Heineke, I., Hoffman, R., 1992. Versuche zur Wirkung des azadirachtenhaltigen formulierten Niemsamextrakte AZT-VR-NR auf Hylobius abietis, Lymantria monacha, und Drino inconspicua. Z. PflKrankh.PflSchutz 99, 337-348.
5. Bodhade, S.N., Borle, M.N., 1985. Sterility effect of some indigenous plant materials on cucurbit fruit-fly. In: Regupathy, A., Jayaraj, S.J. (Eds.), Behavioral and Physiological Approaches in Pest Management. Coimbatore, India, pp. 38-46.
6. Carraway, K.L., Carraway, C.A.C., 1992. The Cytoskeleton: A Practical Approach. Oxford University, Press.
7. Clark, J.I., Garland, D., 1978. Fluorescein colchicine: synthesis, purification and biological activity. J. Cell Biol. 76, 619-627.
8. Cohen, E., Quistad, G.B., Casida, J.E., 1996. Cytotoxicity of nimbolide epoxyazadirachdione and other linonoids from neem insecticide. Life Sci. 58, 1075-1081.
9. Drozdovskaia, L.N., Rapoport, I.A., 1988. Effect of colchicine on Drosophila morphogenesis. Izv. Akad. Nauk. SSSR Biol. 1, 125-128.
10. Dumontet, C., Sikic, B.I., 1999. Mechanisms of action of and resistance to antitubulin agent:Microtubule dynamics, drug transport, and cell death. Journal of Clinical Oncology 17, 1061-1070.
11. Iqbal, S., 1999. The effects of azadirachtin on protein synthesis in specific tissues of the desert locust Schistocerca gregaria. PhD. Thesis, University of Glasgow.
12. Jordan, M.A., Toso, R.J., Thrower, D., 1993. Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proc. Natl. Acad. Sci. USA 90, 9552-9556.
13. Linton, Y.M., Nisbet, A.J., Mordue (Luntz), A.J., 1997. The effects of azadirachtin on the testes of the desert locust, Sch. gregaria (Forskal). J. Insect Physiol. 43, 1077-1084.
14. Little, M., Ludueña, R.F., 1985. Structural differences between brain β1- and β2-tubulins: implications for microtubule assembly and colchicine binding. EMBO J. 4, 51-60.
15. Lowry, O.H., Roseborough, N.L., Farr, A.L., Randall, R.J., 1951. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
16. Ludueña, R., 1998. Multiple forms of tubulin: different gene products and covalent modifications. Int. Revs. Cytol. 178, 207-275.
17. Moll, E., Manz, B., Mocikat, S., Zimmermann, H.-P., 1982. Fluorescent deacetylcolchicine: new aspects of its activity and localization in PtK-1 cells. Exp. Cell Res. 141, 211-220.
18. Mordue (Luntz), A.J., 1998. Azadirachtin - A review of its mode of action in insects. In: Kleeberg, H. (Ed.), Practice Oriented Results on Use and Production of Neem-Ingredients and Pheromones. Druck und Graphic, Wetzlar, Deutschland, pp. 1-4.
19. Morejohn, L.C., Foskett, D.E., 1986. In: Shay, J.W. (Ed.), Cell and Molecular Biology of the Cytoskeleton. Plenum Press, New York, pp. 257-329.
20. Nasioulas, G., Grambitter, K., Himes, R.H.P., Ponstingl, H., 1990. Interaction of a new photosensitive drivative of vinblastine, NAPAVIN, with tubulin and microtubules in vitro. Eur. J. Biochem 192, 69-74.
21. Oxberry, M.E., Geary, T.G., Winterrowd, C.A., Prichard, R.K., 2001. Individual expression of recombinant alpha- and beta-tubulin from Haemonchus contortus: polymerisation and drug effects. Protein Expr. Purif. 21, 30-39.
22. 2] dihydroazadirachtin in Sch. gregaria. J. Insect Physiol. 11, 935-943.
23. Rai, S.S., Wolff, J., 1996. Localisation of the vinblastine-binding site on β-tubulin. J. Biol. Chem. 271, 14707-14711.
24. Reed, E., Majumdar, S.K., 1998. Differential cytotoxic effects of azadirachtin on Spodoptera frugiperda. Entomol. Exp. Applic. 89, 215-221.
25. Rembold, H., Muller, Th., Subrahmanyam, B., 1988. Tissue-specific incorporation of azadirachtin in the malpighian tubules of L. migratoria. Z. Naturforsch 43c, 903-907.
26. Rembold, H., Annadurai, R.S., 1993. Azadirachtin inhibits proliferation SF-9 cells in monolayer culture. Zeitschrift fur Naturforschung C 48, 495-499.
27. Safa, A.R., Hamel, E., Felsted, R.L., 1987. Photoaffinity labelling of of tubulin subunits with a photoactive analogue of vinblastin. Biochemistry 26, 97-102.
28. Salehzadeh, A., Jabbar, A., Jennens, L., Ley, S.V., Annadurai, R.S., Adams, R., Strang, R.H.C., 2002. The effects of phytochemical pesticides on the growth of cultured invertebrate and vertebrate cells. Pest Management Science 58, 268-276.
29. Saxena, R.C., Barrion, A.A., 1987. Cytogenic effects of neem seed kernel extracts (NSKE) on brown planthopper (BPH) Nilaparvata lugens spermatocytes. Int. Rice Res. Newsletter 12, 25-26.
30. Sayah, F., Idaomar, M., Soranzo, L., Karlinsky, A., 1998. Endocrine and neuroendocrine effects of azadirachtin in adult females of the earwig Labidura riparia. Tissue and Cell Res. 30, 86-89.
31. Schimizu, T., 1988. Suppressive effects of azadirachtin on spermiogenesis of the diapausing cabbage armyworm Mamestra brassica, in vitro. Entomol. Exp. Applic. 46, 197-199.
32. Schlüter, U., 1987. Effects of azadirachtin on developing tissues of various insect larvae. In: Schmutterer, H., Ascher, K.R.S. (Eds), Natural Pesticides from the Neem Tree and Other Tropical Plants. Nairobi, Kenya, pp 331-348.
33. Schmutterer, H., Freres, T., 1990. Beeinflussung von Metamorphose, Farbung und Verhalten der Wustenheuschrecke Schistocerca gregaria und derAfricanischen wanderheuschreke Locusta migratoria migratoriodes durch Niemsamenol. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 97, 431-438.
34. Scott, R.H., O'Brien, K., Roberts, L., Mordue, W., Mordue, L.J., 1999. Extracellular and intracellular actions of azadirachtin on the electrophysiological properties of cultured rat DRG neurones. Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol. 123, 85-93.
35. Steets, R., 1975. Die Wirkung von Rohextrakten aus den Meliaceen Azadirachta indica und Melia azadiracht auf verschiedene Insekten. Z. Angew. Entomol. 77, 306-312.
36. Uppuluri, S., Knipling, L., Sackett, D., Wolff, J., 1993. Localisation of the colchicine-binding site of tubulin. Proc. Natl. Acad. Sci. USA 90, 11598-11602.
37. Völlinger, M., 1987. The possible development of resistance against neem NSKE and deltamethrin in Plutella xylostella. In: Schmutterer, H., Ascher, K.R.S. (Eds.), Proc 3rd Int. Neem Conf. Nairobi, Kenya, Natural Pesticides from the Neem Tree., pp. 543-554.
38. Watson, R.D., Ackerman-Morris, S., Smith, W.A., Watson, C.J., Bollenbacher, W.E., 1996. Involvement of microtubules in prothoracicotropic hormone-stimulated ecdysterogenesis by insect (Mandua sexta) prothoracic glands. J. Exp. Zool. 276, 63-69.
39. Williams, R.F., Mumford, C.L., Williams, G.A., Floyd, L.J., Aivaliotis, M.J., Martinez, R.A., 1985. A photoaffinity derivative of colchicine: Photolabeling and location of the colchicine-binding site on the α-subunit of tubulin. J. Biol. Chem. 260, 13794-13802.
40. Wolff, J., Knipling, L., Cahmann, H.J., Palumbo, G., 1991. Direct photoaffinity labelling of tubulin with colchicine. Proc. Natl. Acad. Sci. USA 88, 2820-2824.
41. Yamasaki, R.B., Klocke, J.A., Lee, S.M., Stone, G.A., Darlington, M.V., 1986. Isolation and purification of azadirachtin from neem (Azadirachta indica) seeds using flash chromatography and high-performance liquid chromatography. J. Chromatogr. 356, 220-226.