A high-throughput solid-phase extraction assay capable of measuring diverse polyprenyl phosphate: Sugar-1-phosphate transferases as exemplified by the WecA, MraY, and MurG proteins

Analytical Biochemistry

Volumn 317, Issue 2, 2003, Pages 156-165

  Hyland, Sheryl A ^a   Anderson, Matt S ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Hydrophobic bead; Membrane protein; MraY translocase; MurG transferase; Prenol phosphate:hexosamine 1 phosphate transferase; WecA translocase

Indexed keywords

CATALYSIS; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; ENZYMES; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; LIQUIDS; PHASE SEPARATION;

HYDROPHOBIC BEAD; MEMBRANE PROTEINS; MURG TRANSFERASE; PRENOL PHOSPHATE:HEXOSAMINE-1-PHOSPHATE TRANSFERASE; TRANSLOCASES;

BIOSYNTHESIS;

BACTERIAL PROTEIN; HEXOSAMINE; N ACETYLGLUCOSAMINE; PHOSPHATE; POLYPRENYL PHOSPHATE; PROTEIN MRAY; PROTEIN MURG; PROTEIN WECA; SUGAR PHOSPHATE; TRANSFERASE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; CATALYSIS; CELL WALL; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYMOLOGY; HYDROPHOBICITY; ISOTOPE LABELING; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEPARATION TECHNIQUE; SOLID PHASE EXTRACTION;

BACTERIA (MICROORGANISMS);

EID: 0038296043     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00088-5     Document Type: Article

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