Purification and characterisation of a novel DNA methyltransferase, M.AhdI

Nucleic Acids Research

Volumn 31, Issue 11, 2003, Pages 2803-2810

  Marks, Phil ^c   McGeehan, John ^c   Wilson, Geoff ^a   Errington, Neil ^b   Kneale, Geoff ^c  

^a NEW ENGLAND BIOLABS   (United States)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA METHYLTRANSFERASE; DOUBLE STRANDED DNA; DNA; PROTEIN SUBUNIT;

ARTICLE; BINDING AFFINITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISSOCIATION; DNA BINDING; DNA METHYLATION; DNA SEQUENCE; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBUNIT; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; SEDIMENTATION; STOICHIOMETRY; AMINO ACID SEQUENCE; BACTERIAL GENE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; CONSENSUS SEQUENCE; DIMERIZATION; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; ULTRACENTRIFUGATION;

AMINO ACID SEQUENCE; BINDING SITES; CLONING, MOLECULAR; CONSENSUS SEQUENCE; DIMERIZATION; DNA; DNA MODIFICATION METHYLASES; ESCHERICHIA COLI; GENES, BACTERIAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN SUBUNITS; SEQUENCE ALIGNMENT; ULTRACENTRIFUGATION;

EID: 0038268065     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg399     Document Type: Article

References (23)

1. Dryden,D.T.F. (1999) Bacterial DNA methyltransferases. In Cheng,X. and Blumenthal,R.M. (eds), S-adenosyl Methionine Dependent Methyltransferases: Structure and Function. World Scientific Publishing, New York, NY, pp. 283-340.
2. Murray,N.E. (2000) Type I restriction systems: sophisticated molecular machines. Microbiol. Mol. Biol. Rev., 24, 412-434.
3. Hornby,D.P. (1993) DNA methyltransferases. In Methods in Molecular Biology, Vol. 16: Enzymes of Molecular Biology. Humana Press, Totowa, NJ.
4. Taylor,I., Patel,J., Firman,K. and Kneale,G.G. (1992) Purification and biochemical characterisation of the EcoR124 type I modification methylase. Nucleic Acids Res., 20, 179-186.
5. Dryden,D.T.F., Cooper,L.P. and Murray,N.E. (1993) Purification and characterization of the methyltransferase from the Type-1 restriction and modification system of E.coli K12. J. Biol. Chem., 268, 13228-13236.
6. Kneale,G.G. (1994) A symmetrical model for the domain structure of Type I DNA methyltransferases. J. Mol. Biol., 243, 1-5.
7. Mernagh,D.R., Janscak,P., Firman,K. and Kneale,G.G. (1998) Protein-protein and protein-DNA interactions in the Type I restriction endonuclease R.EcoR124I. Biol. Chem., 379, 497-504.
8. Janscak,P., Dryden,D.T.F. and Firman,K. (1998) Analysis of the subunit assembly of the type IC restriction-modification enzyme EcoR124I. Nucleic Acids Res., 26, 4439-4445.
9. Dryden,D.T., Cooper,L.P., Thorpe,P.H. and Byron,O. (1997) The in vitro assembly of the EcoKI type I DNA restriction/modification enzyme and its in vivo implications. Biochemistry, 36, 1065-1076.
10. Cowan,G.M., Gann,A.A.F. and Murray,N.E. (1989) Conservation of complex recognition domains between families of restriction enzymes. Cell, 56, 103-109.
11. Gubler,M., Braguglia,D., Meyer,J. Piekarowitz,A. and Bickle,T.A. (1992) Recombination of constant and variable modules alters DNA sequence recognition by Type IC restriction-modification enzymes. EMBO J., 11, 233-240.
12. Cui,T., Lunnen,K., Chang,Z., Morgan,R., Nwanko,D., Slatko,B. and Wilson,G. (1997) Type 1 1/2 R-M systems. In Proceedings of the 4th NEB Workshop on Biological DNA Modification, Igls, Tyrol, Austria.
13. Marks,P. (2001) Cloning expression and characterisation of the novel DNA methyltransferase, M.AhdI. PhD thesis, University of Portsmouth, Portsmouth, UK.
14. Mernagh,D.R., Reynolds,L.A. and Kneale,G.G. (1997) DNA binding and subunit interactions in the type I methyltransferase M.EcoR124I. Nucleic Acids Res., 25, 987-991.
15. Philo,J.S. (2000) A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Anal. Biochem., 279, 151-163.
16. Abadjieva,A., Patel,J., Webb,M., Zinkevich,V. and Firman,K. (1993) A deletion mutant of the type 1C restriction endonuclease EcoR124I expressing a novel DNA specificty. Nucleic Acids Res., 21, 4435-4443.
17. Meister,J., MacWilliams,M., Hubner,P., Jutte,H., Skrzypek,E., Piekarowicz,A. and Bickle,T.A. (1993) Macroevolution by transposition: drastic modification of DNA recognition by a type I restriction enzyme following Tn5 transposition. EMBO J., 12, 4585-4591.
18. Smith,M.A., Mernagh,D.R. and Kneale,G.G. (1998) Expression and characterisation of the N-terminal fragment of the HsdS subunit of M.EcoR124I. Biol. Chem., 379, 505-510.
19. Smith,M., Read,C.M. and Kneale,G.G. (2001) Domain structure and subunit interactions in the type I DNA methyltransferase M.EcoR124I. J. Mol. Biol., 314, 41-50.
20. Rubin,R.A. and Modrich,P. (1977) EcoRI methylase: physical and catalytic properties of the homogenous enzyme. J. Biol. Chem., 252, 7265-7272.
21. Reich,N.O. and Mashhoon,N. (1991) Kinetic mechanism of EcoRI DNA methyltransferase. Biochemistry, 30, 2933-2929.
22. Cooper,L.P. and Dryden,D.T.F. (1994) The domains of a type I DNA methyltransferase: interactions and role in recognition of DNA methylation. J. Mol. Biol., 236, 1011-1021.
23. Janscak,P. and Bickle,T.A. (1998) The DNA recognition subunit of the type IB restriction-modification enzyme EcoAI tolerates circular permutations of its polypeptide chain. J. Mol. Biol., 284, 937-948.