Construction of strains for rapid homokaryon purification after integration of constructs at the histidine-3 (his-3) locus of Neurospora crassa

Current Genetics

Volumn 43, Issue 1, 2003, Pages 17-23

  Lee, Dong W ^a   Haag, Jeremy R ^b   Aramayo, Rodolfo ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

Counterselection; Gene replacement; Homokaryon purification; Homologous integration; Marker eviction

Indexed keywords

AMPICILLIN; HISTIDINE; HYGROMYCIN B; KANAMYCIN; PHOSPHOTRANSFERASE; PLASMID VECTOR; THYMIDINE KINASE;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; FUNGAL STRAIN; GENE CONSTRUCT; GENE FUSION; GENE IDENTIFICATION; GENE LOCUS; GENE TARGETING; GENETIC ANALYSIS; GENETIC TRANSFORMATION; HERPES SIMPLEX VIRUS; NEUROSPORA CRASSA; NONHUMAN; NUCLEOTIDE SEQUENCE; PENICILLIN RESISTANCE; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ANIMALIA; ESCHERICHIA COLI; NEUROSPORA; NEUROSPORA CRASSA; SIMPLEXVIRUS;

EID: 0038266846     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-002-0366-z     Document Type: Article

References (20)

1. Aramayo R, Metzenberg RL (1996a) Gene replacements at the his-3 locus of Neurospora crassa. Fungal Genet Newsl 43:9-13
2. Aramayo R, Metzenberg RL (1996b) Meiotic transvection in fungi. Cell 86:103-113
3. +, a Neurospora crassa gene related to transcriptional regulators of fungal development. Genetics 144:991-1003
4. Brockman HE, Serres FJ de (1963) "Sorbose toxicity" in Neurospara. Am J Bot 50:709-714
5. Davis RH, Serres FJ de (1970) Genetic and microbiological research techniques for Neurospora crassa. in: Colowick SP, Kaplan NO (eds) Metabolism of amino acids and amines. Academic Press, New York, pp 79-143
6. Ebbole D (1990) Vectors for construction of translational fusions to β-galactosidase. Fungal Genet Newsl 37:15
7. Grant DM, Lambowitz AM, Rambosek JA, Kinsey JA (1984) Transformation of Neurospora crassa with recombinant plasmids containing the cloned glutamate dehydrogenase (am) gene: evidence for autonomous replication of the transforming plasmid. Mol Cell Biol 4:2041-2051
8. Lupton SD, Brunton LL, Kalberg VA, Overell RW (1991) Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene. Mol Cell Biol 11:3374-3378
9. Margolin BS, Freitag M, Selker EU (1997) Improved plasmids for gene targeting at the his-3 locus of Neurospora crassa by electroporation. Fungal Genet Newsl 44:34-36
10. Orbach MJ, Porro EB, Yanofsky C (1986) Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol 6:2452-2461
11. Perkins DD, Kinsey JA, Asch DK, Frederick GD (1993) Chromosome rearrangements recovered following transformation of Neurospora crassa. Genetics 134:729-736
12. Pratt RJ, Aramayo R (2002) Improving the efficiency of gene replacements in Neurospora crassa: a first step towards a large-scale functional genomics project. Fungal Genet Biol. 37:56-71
13. Pridmore RD (1987) New and versatile cloning vectors with kanamycin-resistance marker. Gene 56:309-312
14. Sachs MS, Ebbole D (1990) The use of lacZ gene fusions in Neurospora crassa. Fungal Genet Newsl 37:35-36
15. Sachs MS, et al (1997) Expression of herpes virus thymidine kinase in Neurospora crassa. Nucleic Acids Res 25:2389-2395
16. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. Selker EU (1990) Premeiotic instability of repeated sequences in Neurospora crassa. Annu Rev Genet 24:579-613
18. Selker EU, Stevens JN (1987) Signal for DNA methylation associated with tandem duplication in Neurospora crassa. Mol Cell Biol 7:1032-1038
19. Selker EU, Cambareri EB, Jensen BC, Haack KR (1987) Rearrangement of duplicated DNA in specialized cells of Neurospora. Cell 51:741-752
20. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119