Comparative studies with penicillinase, horseradish peroxidase, and alkaline phosphatase as enzyme labels in developing enzyme immunoassay of cortisol

Journal of Immunoassay and Immunochemistry

Volumn 24, Issue 2, 2003, Pages 173-190

  Kumari, G Lakshmi ^a   Dhir, Ravindra N ^a,b  

^a Hormone Research Foundation   (India)

^b UNIVERSITY OF PENNSYLVANIA SCHOOL OF VETERINARY MEDICINE   (United States)

Author keywords

Alkaline phosphatase; ALP; ELISA of cortisol; Horseradish peroxidase; HRP; Penicillinase

Indexed keywords

3 O (CARBOXYMETHYL)OXIME; 8 ANILINO 1 NAPHTHALENESULFONIC ACID; ALKALINE PHOSPHATASE; BOVINE SERUM ALBUMIN; BUFFER; HORSERADISH PEROXIDASE; HYDROCORTISONE; IMMUNOGLOBULIN ANTIBODY; PENICILLINASE; RABBIT ANTISERUM; REAGENT; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CONCENTRATION RESPONSE; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FREEZING; HUMAN; HUMAN TISSUE; HYDROCORTISONE BLOOD LEVEL; INTERMETHOD COMPARISON; MALE; NORMAL HUMAN; PERFORMANCE; PRIORITY JOURNAL; REACTION ANALYSIS; REACTION TIME; STANDARD; THAWING; TUBE;

ALKALINE PHOSPHATASE; ANILINO NAPHTHALENESULFONATES; ANIMALS; ANTIBODY SPECIFICITY; CATTLE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG STABILITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GOATS; HORSERADISH PEROXIDASE; HUMANS; HYDROCORTISONE; MALE; PENICILLINASE; RABBITS; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SERUM ALBUMIN, BOVINE;

EID: 0038246336     PISSN: 15321819     EISSN: None     Source Type: Journal    
DOI: 10.1081/IAS-120020083     Document Type: Article

References (13)

1. Comoglio, S.; Celada, F. An immuno-enzymatic assay of cortisol using E. coli β-galactosidase as label. J. Immunol. Meth. 1976, 10, 161-170.
2. Ogihara, T.; Miyai, F.; Nishi, K.; Ishibashi, K.; Kumahara, Y. Enzyme labeled immunoassay for plasma cortisol. J. Clin. Endocrinol. Metab. 1977, 44, 91-95.
3. Lewis, J.G.; Elder, P.A. An enzyme linked immunosorbent assay (ELISA) for plasma cortisol. J. Steroid Biochem. 1985, 221, 673-676.
4. Shrivastav, T.G.; Kumari, G.L.; Rao, P.N. Enzyme immunoassay of cortisol in human plasma using penicillinase as label. Clin. Chim. Acta 1988, 174, 83-92.
5. Basu, A.; Shrivastav, T.G. One step enzyme linked immunosorbent assay for direct estimation of serum cortisol. J. Immunoassay 2000, 21, 39-50.
6. Khatkhatay, M.I.; Desai, M.A. Comparison of performances of four enzyme to β-lactamase. J. Immunoassay 1999, 20, 151-183.
7. Portsmann, B.; Portsmann, T.; Nugel, E.; Evers, U. Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase or β-galactosidase? J. Immunol. Meth. 1985, 79, 27-37.
8. Hosoda, H.; Takasaki, W.; Tsukamoto, R.; Nambara, J. Sensitivity of steroid enzyme immunoassays. Comparison of alkaline phosphatase, β-glactosidase and horseradish peroxidase as labels in a colorimetric assay system. Chem. Pharm. Bull. 1987, 35, 3336-3342.
9. Sauer, M.J.; Foulkes, J.A.; O'Neill, P.M. A comparison of alkaline phosphatase, β-galactosidase, penicillinase and peroxidase used as labels for progesterone determination in milk by heterologous microtitre plate enzyme immunoassay. J. Steroid Biochem. 1989, 33, 423-431.
10. Levy, H.B.; Sober, S.A. A simple chromatographic method of preparation of gamma globulin. Proc. Soc. Exp. Biol. Med. 1960, 103, 250-252.
11. Mattox, V.R.; Litwiller, R.D.; Nelson, A.N. A comparison procedure for attaching steroidal glucosiduronic acid to bovine serum albumin. J. Steroid Biochem. 1979, 10, 167-172.
12. Panday, P.K.; Shrivastav, T.G.; Kumari, G.L.; Rao, P.N.; Grover, P.K.; Murthy, H.G.K. Enzyme immunosorbent assay of oestradiol in unextracted plasma using penicillinase as label. Clin. Chem. Acta 1990, 190, 175-184.
13. Joshi, U.; Raghavan, V.; Zemse, G.; Sheth, A.; Borkar, P.S.; Ramachandran, S. Use of 'Penicillinase' in the estimation of human chorionic gonadotrophin and human placental lactogen by enzyme-linked immunoassays. In Enzyme-Linked Immunoassay of Hormones and Drugs; Pal, S.B., de Gruyter, W., Eds; Berlin, 1987; 211-245.