Direct observation of substrate induction of resistance mechanism in Pseudomonas aeruginosa using single live cell imaging

Biochemical and Biophysical Research Communications

Volumn 305, Issue 4, 2003, Pages 941-949

  Xu, Xiao Hong Nancy ^a   Wan, Qian ^a   Kyriacou, Sophia V ^a   Brownlow, William J ^a   Nowak, Michelle E ^a  

^a OLD DOMINION UNIVERSITY   (United States)

Author keywords

Dark field microscopy; Efflux pump; Ethidium bromide; Fluorescence microscopy and spectroscopy; Live cell imaging; Multidrug resistance; Pseudomonas aeruginosa

Indexed keywords

BUFFER; ETHIDIUM BROMIDE;

ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BIOACCUMULATION; CELL; CHRONOSEQUENCE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEGRADATION; ENZYME SUBSTRATE; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; IMAGING; INCUBATION TIME; MICROBIAL ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0038242346     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)00874-X     Document Type: Article

References (28)

1. Poole K. Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms. J. Mol. Microbiol. Biotechnol. 3(2):2001;255-264.
2. Nakae T. Multiantibiotic resistance caused by active drug extrusion in Pseudomonas aeruginosa and other gram-negative bacteria. Microbiologia. 13(3):1997;273-284. and reference therein.
3. For review: Byan B.M., Dougherty T.J., Beaulieu D., Chuang J., Dougherty B.A., Barrett J.F. Efflux in bacteria: what do we really know about it? Expert Opin. 10(7):2001;1409-1422. and reference therein.
4. Cole S.P., Bhardwaj G., Gerlach J.H., Mackie J.E., Grant C.E., Almquist K.C., Stewart A.J., Kurz E.U., Duncan A.M., Deeley R.G. Overexpression of a transporter gene in a mutidrug-resistant human lung cancer cell line. Science. 258:1992;1650-1654.
5. Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R., Smith K., Spencer D., Wong G.K., Wu Z., Paulsen I.T. Complete genome sequences of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature. 406:2000;959-964.
6. Ma D., Cook D.N., Hearst J.E., Nikaido H. Efflux pumps and drug resistance in gram-negative bacteria. Trends Microbiol. 2(12):1994;489-493.
7. Masuda N., Sakagawa E., Ohya S., Gotoh N., Tsujimoto H., Nishino T. Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44(12):2000;3322-3327.
8. Maseda H., Yoneyama H., Nakae T. Assignment of the substrate-selective subunits of the MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44(3):2000;658-664.
9. Morshed S.R., Lei Y., Yoneyama H., Nakae T. Expression of genes associated with antibiotic extrusion in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 210(2):1995;356-362.
10. Lee A., Mao W., Warren M.S., Mistry A., Hoshino K., Okumura R., Ishida H., Lomovskaya O. Interplay between efflux pumps may provide either additive or multiplicative effects on drug resistance. J. Bacteriol. 182(11):2000;3142-3150.
11. Germ M., Yoshihara E., Yoneyama H., Nakae T. Interplay between the efflux pump and the outer membrane permeability barrier in fluorescent dye accumulation in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 261(2):1999;452-455.
12. For review: Nakae T. Role of membrane permeability in determining antibiotic resistance in Pseudomonas aeruginosa. Microbiol. Immunol. 39(4):1995;221-229. and reference therein.
13. Lomovskaya O., Warren M.S., Lee A., Galazzo J., Fronko R., Lee M., Blais J., Cho D., Chamberland S., Renau T., Leger R., Hecker S., Watkins W., Hoshino K., Ishida H., Lee V.J. Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob. Agents Chemother. 45(1):2001;105-116.
14. Mortimer P.G., Piddock L.J. A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus. J. Antimicrob. Chemother. 28(5):1991;639-653.
15. Ocaktan A., Yoneyama H., Nakae T. Use of fluorescence probes to monitor function of the subunit proteins of the MexA-MexB-oprM drug extrusion machinery in Pseudomonas aeruginosa. J. Biol. Chem. 272(35):1997;21964-21969.
16. Yoneyama H., Maseda H., Kamiguchi H., Nakae T. Function of the membrane fusion protein, MexA, of the MexA, B-OprM efflux pump in Pseudomonas aeruginosa without an anchoring membrane. J. Biol. Chem. 275(7):2000;4628-4634.
17. Kyriacou S.V., Nowak M.E., Brownlow W.J., Xu X.-H.N. Single live cell imaging for real-time monitoring of resistance mechanism in Pseudomonas aeruginosa. J. Biomed. Opt. 7(4):2002;576-586.
18. Morjani H., Aouali N., Belhoussine R., Veldman R.J., Levade T., Manfait M. Elevation of glucosylceramide in multidrug-resistant cancer cells and accumulation in cytoplasmic droplets. Int. J. Cancer. 94(2):2001;157-165. and reference therein.
19. Crivellato E., Candussio L., Rosati A.M., Bartoli-Klugmann F., Mallardi F., Decorti G. The fluorescence probe bodipy-FL-verapamil is a substrate for both P-glycoprotein and multidrug resistance-related protein (MRP)-1. J. Histochem. Cytochem. 50(5):2002;731-734. and reference therein.
20. Xu X.-H.N., Chen J., Jeffers R., Kyriacou S. Direct measurement of sizes and dynamics of single living membrane transporters using nano-optics. Nano Letters. 2:2002;175-182.
21. X.-H.N. Xu, W. Brownlow, S. Huang, J. Chen, Single molecule detection of efflux pump machinery in Pseudomonas aeruginosa, Biochem. Biophys. Res. Commun. (in press).
22. S.V. Kyriacou, Real-time study of multidrug resistance mechanism in Pseudomonas aeruginosa using nanoparticle optics and single live cell imaging, Thesis, Old Dominion University, 2003.
23. Saito K., Yoneyama H., Nakae T. nalB-type mutations causing the overexpression of the MexAB-OprM efflux pump are located in mexR gene of the Pseudomonas aeruginosa chromosome. FEMS Microbiol. Lett. 179(1):1999;67-72.
24. Yoneyama H., Ocaktan A., Tsuda M., Nakae T. The role of mex-gene products in antibiotic extrusion in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 233:1997;611-618.
25. Greenwood D. Modes of action. O'grady F., Lambert H.P., Finch R.G., Greenwood D. Antibiotic and Chemotherapy. seventh ed. 1997;10-21 Churchill Livingstone, New York.
26. Morgan A.R., Lee J.S., Pulleyblank D.E., Murray N.L., Evans D.H. Review: ethidium fluorescence assays. Part 1. Physicochemical studies. Nucleic Acids Res. 7(3):1979;547-569.
27. Freifelder D. Physical Biochemistry. 1982;51-52 Freeman, New York.
28. Mie G. Beiträg zur Optik trüber Medien, speziell kolloidaler Metrallösungen. Ann. Phys. 25:1908;377-445.