Quorum sensing of Rhodobacter sphaeroides negatively regulates cellular poly-β-Hydroxybutyrate content under aerobic growth conditions

Journal of Microbiology and Biotechnology

Volumn 13, Issue 3, 2003, Pages 477-481

  Kho, Dhong Hyo ^a   Jang, Ji Hee ^a   Kim, Hye Sun ^a   Kim, Kun Soo ^a   Lee, Jeong K ^a  

^a Sogang University   (South Korea)

Author keywords

cer operon; PHB synthase; phbC; Quorum sensing; Rhodobacter sphaeroides

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; BETA GALACTOSIDASE; POLY(3 HYDROXYBUTYRIC ACID); REGULATOR PROTEIN; SYNTHETASE;

AEROBIC BACTERIUM; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GROWTH; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; GENE DELETION; GENE INSERTION; GENETIC CODE; NONHUMAN; OPEN READING FRAME; OPERON; RHODOBACTER SPHAEROIDES; TRANSCRIPTION REGULATION; WILD TYPE;

BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; PROKARYOTA; RHODOBACTER; RHODOBACTER SPHAEROIDES; RHODOPSEUDOMONAS;

EID: 0038235923     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Anderson, J. A. and E. A. Dawes. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54: 450- 472.
2. DeHoff, B. S., J. K. Lee, T. J. Donohue, R. I. Gumport, and S. Kaplan. 1988. In vivo analysis of puf operon expression in Rhodobacter sphaeroides after deletion of a putative intercistronic transcription terminator. J. Bacteriol. 170: 4681-4692.
3. Fuqua, W. C. and S. C. Winans. 1994. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J. Bacteriol. 176: 2796-2806.
4. Fuqua, W. C., S. C. Winans, and E. P. Greenberg. 1994. Quorum sensing in bacteria: The LuxR-LuxI family of cell density-responsive transcriptional regulators. J. Bacteriol. 176: 269-275.
5. Hustede, E. and A. Steinbühel. 1993. Characterization of the polyhydroxyalkanoate synthase gene locus of Rhodobacter sphaeroides. Biotechnol. Lett. 15: 709-714.
6. Kho, D. H., C. Y. Jeong, and J. K. Lee. 2001. Expression analysis of β-ketothiolase and acetoacetyl-CoA reductase of Rhodobacter sphaeroides. J. Microbiol. Biotechnol. 11: 1031-1037.
7. Kho, D. H., J. M. Yang, K. S. Kim, and J. K. Lee. 2001. Expression analysis of phbC coding for poly-3-hydroxybutyrate (PHB) synthase of Rhodobacter sphaeroides. J. Microbiol. Biotechnol. 11: 310-316.
8. Kiley, P. J. and S. Kaplan. 1988. Molecular genetics of photosynthetic membrane biosynthesis in Rhodobacter sphaeroides. Microbiol. Rev. 52: 50-69.
9. Law, J. H. and R. A. Slepecky. 1961. Assay of poly-3-hydroxybutyric acid. J. Bacteriol. 82: 33-36.
10. Liebergesell, M., E. Hustede, A. Timm, A. Steinbühel, R. C. Fuller, and R. W. Lenz. 1991. Formation of poly (3-hydroxyalkanoates) by phototrophic and chemolithotrophic bacteria. Arch. Microbiol. 155: 415-421.
11. Lueking, D. R., R. T. Fralely, and S. Kaplan. 1978. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodobacter sphaeroides. J. Biol. Chem. 253: 451-457.
12. Miyake, M., K. Kataoka, M. Shirai, and Y. Asada. 1997. Control of poly-β-hydroxybutyrate synthase mediated by acetyl phosphate in Cyanobacteria. J. Bacterol. 176: 5009-5013.
13. Miyamoto, C. M., J. Chatterjee, E. Swartzman, R. Szittner, and E. A. Meighen. 1996. The role of the lux autoinducer in regulating luminescence in Vibrio harveyi; control of luxR expression. Mol. Microbiol. 19: 767-775.
14. Miyamoto, C. M., S. Weiqun, and E. A. Meighen. 1998. The LuxR regulator protein controls synthesis of polyhydroxybutyrate in Vibrio harveyi. Biochim. Biophys. Acta 1384: 356-364.
15. Pearson, J. P., K. M. Gray, L. Passador, K. D. Tucker, A. Eberhard, B. H. Iglewski, and E. P. Greenberg. 1994. Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc. Natl. Acad. Sci. USA 91: 197-201.
16. Peoples, O. P. and A. J. Sinskey. 1989. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. J. Biol. Chem. 264: 15298-15303.
17. Puskas, A., E. P. Greenberg, S. Kaplan, and A. L. Schaefer. 1997. A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides. J. Bacteriol. 179: 7530-7537.
18. Sasakawa, C. and M. Yoshikawa. 1987. A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Gene 56: 283-288.
19. Simon, R., V. Priefer, and A. Puhler. 1983. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram negative bacteria. Bio/Technology 1: 784-791.