Are readily culturable bacteria in coastal north sea waters suppressed by selective grazing mortality?

Applied and Environmental Microbiology

Volumn 69, Issue 5, 2003, Pages 2624-2630

  Beardsley, Christine ^a   Pernthaler, Jakob ^a   Wosniok, Werner ^b   Amann, Rudolf ^a  

^a MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY   (Germany)

^b UNIVERSITY OF BREMEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; FILTERS (FOR FLUIDS); PROTEINS; SEAWATER;

HETEROTROPHIC NANOFLAGELLATES (HNF);

MICROBIOLOGY;

SEA WATER;

BACTERIUM; GRAZING; INTERSPECIFIC INTERACTION; MARINE ENVIRONMENT; MORTALITY; PICOPLANKTON;

ALTEROMONAS; ARTICLE; ATLANTIC OCEAN; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIUM CULTURE; BIOFILTRATION; CONTROLLED STUDY; FLAGELLATE; FLEXIBACTERACEAE; GRAM NEGATIVE BACTERIUM; GRAZING; NONHUMAN; PROTEIN CONTENT; PSEUDOALTEROMONAS; STATISTICAL ANALYSIS; VIBRIO;

ALTEROMONAS; ANIMALS; BACTERIA; BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; ECOSYSTEM; GAMMAPROTEOBACTERIA; GERMANY; MASTIGOPHORA; PSEUDOALTEROMONAS; SEAWATER; VIBRIO; ZOOPLANKTON;

NORTH SEA;

ALTEROMONAS; BACTERIA (MICROORGANISMS); CYTOPHAGA LATERCULA; CYTOPHAGA MARINOFLAVA; FLEXIBACTERACEAE; MASTIGOPHORA (FLAGELLATES); NEGIBACTERIA; PROTEOBACTERIA; PSEUDOALTEROMONAS; ROSEOBACTER; VIBRIO;

EID: 0038221079     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.5.2624-2630.2003     Document Type: Article

References (53)

1. Button, D. K., B. R. Robertson, P. W. Lepp, and T. M. Schmidt. 1998. A small, dilute-cytoplasm, high-affinity, novel bacterium isolated by extinction culture and having kinetic constants compatible with growth at ambient concentrations of dissolved nutrients in seawater. Appl. Environ. Microbiol. 64:4467-4476.
2. Cottrell, M. T., and D. L. Kirchman. 2000. Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl. Environ. Microbiol. 66:5116-5122.
3. Daims, H., A. Bruhl, R. Amann, K. H. Schleifer, and M. Wagner. 1999. The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol. 22:434-444.
4. Del Giorgio, P. A., J. M. Gasol, D. Vaque, P. Mura, S. Agusti, and C. M. Duarte. 1996. Bacterioplankton community structure: protists control net production and the propoportion of active bacteria in a coastal marine community. Limnol. Oceanogr. 41:1169-1179.
5. Eilers, H., J. Pernthaler, and R. Amann. 2000. Succession of pelagic marine bacteria during enrichment: a close look on cultivation-induced shifts. Appl. Environ. Microbiol. 66:4634-4640.
6. Eilers, H., J. Pernthaler, F. O. Glöckner, and R. Amann. 2000. Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66:3044-3051.
7. Eilers, H., J. Pernthaler, J. Peplies, F. O. Glöckner, G. Gerdts, and R. Amann. 2001. Isolation of novel pelagic bacteria from the German Bight and their seasonal contribution to surface picoplankton. Appl. Environ. Microbiol. 67:5134-5142.
8. Ferguson, R. L., E. N. Buckley, and A. V. Palumbo. 1984. Response of marine bacterioplankton to differential filtration and confinement. Appl. Environ. Microbiol. 47:49-55.
9. Flärdh, K., P. S. Cohen, and S. Kjelleberg. 1992. Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956. J. Bacteriol. 174:6780-6788.
10. Fuchs, B. M., M. V. Zubkov, K. Sahm, P. H. Burkill, and R. Amann. 2000. Changes in community composition during dilution cultures of marine bacterioplankton as assessed by flow cytometric and molecular biological techniques. Environ. Microbiol. 2:191-201.
11. Fuhrman, J. A., K. McCallum, and A. A. Davis. 1992. Novel major archaebacterial group from marine plankton. Nature 356:148-149.
12. Fuhrman, J. A., and R. T. Noble. 1995. Viruses and protists cause similar bacterial mortality in coastal seawaters. Limnol. Oceanogr. 40:1236-1242.
13. Garcia-Martinez, J., S. G. Acinas, R. Massana, and F. Rodriguez-Valera. 2002. Prevalence and microdiversity of Alteromonas macleodii-like microorganisms in different oceanic regions. Environ. Microbiol. 4:42-50.
14. Gasol, J. M., and X. A. G. Morán. 1999. Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry. Aquat. Microb. Ecol. 16:251-264.
15. Giovannoni, S. J., T. B. Britschgi, C. L. Moyer, and K. G. Field. 1990. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345:60-63.
16. Giuliano, L., E. De Domenico, M. G. Höfle, and M. M. Yakimov. 1999. Identification of culturable oligotrophic bacteria within naturally occurring bacterioplankton communities of the Ligurian Sea by 16S rRNA sequencing and probing. Microb. Ecol. 37:77-85.
17. Glöckner, F. O., R. Amann, A. Alfreider, J. Pernthaler, R. Psenner, K. Trebesius, and K.-H. Schleifer. 1996. An in situ hybridization protocol for detection and identification of planktonic bacteria. Syst. Appl. Microbiol. 19:403-406.
18. Goldman, J. C., and M. R. Dennett. 1985. Susceptibility of some marinephytoplankton species to cell breakage during filtration and post-filtration rinsing. J. Exp. Mar. Biol. Ecol. 86:47-58.
19. Gonzalez, J. M., and M. A. Moran. 1997. Numerical dominance of a group of marine bacteria in the alpha-subclass of the class Proteobacteria in coastal seawater. Appl. Environ. Microbiol. 63:4237-4242.
20. Gonzalez, J. M., E. B. Sherr, and B. F. Sherr. 1990. Size-selective grazing on bacteria by natural assemblages of estuarine flagellates and ciliates. Appl. Environ. Microbiol. 56:583-589.
21. Hahn, M. W., and M. G. Hofle. 2001. Grazing of protozoa and its effect on populations of aquatic bacteria. FEMS Microbiol. Ecol. 35:113-121.
22. Hahn, M. W., and M. G. Höfle. 1999. Flagellate predation on a bacterial model community: interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition. Appl. Environ. Microbiol. 65:4863-4872.
23. Heidelberg, J. F., K. B. Heidelberg, and R. R. Colwell. 2002. Seasonality of Chesapeake Bay bacterioplankton species. Appl. Environ. Microbiol. 68:5488-5497.
24. Jürgens, K., and C. Matz. 2002. Predation as a shaping force for the phenotypic and genotypic composition of planktonic bacteria. Antonie Leeuwenhoek Int. J. Gen. Mol. Microbiol. 81:413-434.
25. Jürgens, K., J. Pernthaler, S. Schalla, and R. Amann. 1999. Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing. Appl. Environ. Microbiol. 65:1241-1250.
26. Manz, W., R. Amann, W. Ludwig, M. Wagner, and K.-H. Schleifer. 1992. Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15:593-600.
27. Massana, R., C. Pedros-Alio, E. O. Casamayor, and J. M. Gasol. 2001. Changes in marine bacterioplankton phylogenetic composition during incubations designed to measure biogeochemically significant parameters. Limnol. Oceanogr. 46:1181-1188.
28. Monger, B. C., and M. R. Landry. 1991. Prey-size dependency of grazing by free-living marine flagellates. Mar. Ecol. Prog. Ser. 74:239-248.
29. Pernthaler, A., J. Pernthaler, and R. Amann. 2002. Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria. Appl. Environ. Microbiol. 68:3094-3101.
30. Pernthaler, A., J. Pernthaler, H. Eilers, and R. Amann. 2001. Growth patterns of two marine isolates: adaptations to substrate patchiness? Appl. Environ. Microbiol. 67:4077-4083.
31. Pernthaler, J., F. O. Glöckner, W. Schönhuber, and R. Amann. 2001. Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes. Methods Microbiol. 30:207-226.
32. Pernthaler, J., T. Posch, K. Simek, J. Vrba, R. Amann, and R. Psenner. 1997. Contrasting bacterial strategies to coexist with a flagellate predator in an experimental microbial assemblage. Appl. Environ. Microbiol. 63:596-601.
33. Pernthaler, J., T. Posch, K. Simek, J. Vrba, A. Pernthaler, F. O. Glöckner, U. Nübel, R. Psenner, and R. Amann. 2001. Predator-specific enrichment of actinobacteria from a cosmopolitan freshwater clade in mixed continuous culture. Appl. Environ. Microbiol. 67:2145-2155.
34. Pinhassi, J., U. L. Zweifel, and A. Hagström. 1997. Dominant marine bacterioplankton species found among colony-forming bacteria. Appl. Environ. Microbiol. 63:3359-3366.
35. Posch, T., M. Loferer-Krossbacher, G. Gao, A. Alfreider, J. Pernthaler, and R. Psenner. 2001. Precision of bacterioplankton biomass determination: a comparison of two fluorescent dyes and of allometric and linear volume-to-carbon conversion factors. Aquat. Microb. Ecol. 25:55-63.
36. Posch, T., J. Pernthaler, A. Alfreider, and R. Psenner. 1997. Cell-specific respiratory activity of aquatic bacteria studied with the tetrazolium reduction method, cyto-clear slides and image analysis. Appl. Environ. Microbiol. 63:867-873.
37. Rappe, M. S., S. A. Connon, K. L. Vergin, and S. J. Giovannoni. 2002. Cultivation of the ubiquitous SAR11 marine bacterioplankton clade. Nature 418:630-633.
38. Sanders, R. W., D. A. Caron, and U. G. Berninger. 1992. Relationship between bacteria and heterotrophic nanoplankton in marine and freshwaters: an inter-ecosystem comparison. Mar. Ecol. Prog. Ser. 86:1-14.
39. Schäfer, H., P. Servais, and G. Muyzer. 2000. Successional changes in the genetic diversity of a marine assemblage during confinement. Arch. Microbiol. 173:138-145.
40. Sherr, B. F., E. B. Sherr, and C. Pedros Alio. 1989. Simultaneous measurement of bacterioplankton production and protozoan bacterivory in estuarine water. Mar. Ecol. Prog. Ser. 54:209-219.
41. Sherr, E. B., and B. F. Sherr. 1993. Protistan grazing rates via uptake of fluorescently labelled prey, p. 695-701. In P. Kemp, B. F. Sherr, E. B. Sherr, and J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis, Boca Raton, Fla.
42. Sherr, E. B., B. F. Sherr, and C. T. Sigmon. 1999. Activity of marine bacteria under incubated and in situ conditions. Aquat. Microb. Ecol. 20:213-223.
43. Simek, K., and T. H. Chrzanowski. 1992. Direct and indirect evidence of size-selective grazing on pelagic bacteria by freshwater nanoflagellates. Appl. Environ. Microbiol. 58:3715-3720.
44. Simek, K., J. Pernthaler, M. Weinbauer, K. Hornak, J. Dolan, J. Nedoma, M. Masin, and R. Amann. 2001. Changes in bacterial community composition, dynamics and viral mortality rates associated with enhanced flagellate grazing in a mesoeutrophic reservoir. Appl. Environ. Microbiol. 67:2723-2733.
45. Simek, K., J. Vrba, J. Pernthaler, T. Posch, P. Hartman, J. Nedoma, and R. Psenner. 1997. Morphological and compositional shifts in an experimental bacterial community influenced by protists with contrasting feeding modes. Appl. Environ. Microbiol. 63:587-595.
46. Simon, M., H. P. Grossart, B. Schweitzer, and H. Ploug. 2002. Microbial ecology of organic aggregates in aquatic ecosystems. Aquat. Microb. Ecol. 28:175-211.
47. Sonnemann, E. 1982. Allgemeine Lösungen multipler Testprobleme. EDV Med. Biol. 13:120-128.
48. Suzuki, M. T. 1999. Effect of protistan bacterivory on coastal bacterioplankton diversity. Aquat. Microb. Ecol. 20:261-272.
49. Tansley, A. G., and R. S. Adamson. 1925. Studies of the vegetation of the English chalk III: the chalk grasslands of Hampshire-Sussex border. J. Ecol. 13:177-223.
50. Thingstad, T. F. 2000. Elements of a theory for the mechanisms controlling abundance, diversity, and biogeochemical role of lyric bacterial viruses in aquatic systems. Limnol. Oceanogr. 45:1320-1328.
51. Wichels, A., S. S. Biel, H. R. Gelderblom, T. Brinkhoff, G. Muyzer, and C. Schütt. 1998. Bacteriophage diversity in the North Sea. Appl. Environ. Microbiol. 64:4128-4133.
52. Zubkov, M. V., B. M. Fuchs, S. D. Archer, R. P. Kiene, R. Amann, and P. A. Burkill. 2001. Linking the composition of bacterioplankton to rapid turnover of dissolved dimethylsulfoniopropionate in an algal bloom in the North Sea. Environ. Microbiol. 3:304-311.
53. Zubkov, M. V., B. M. Fuchs, H. Eilers, P. H. Burkill, and R. Amann. 1999. Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry. Appl. Environ. Microbiol. 65:3251-3257.