Expression, purification and characterization of recombinant human microsomal PGE2 synthase-1

Advances in Experimental Medicine and Biology

Volumn 525, Issue , 2003, Pages 113-116

  Ouellet, Marc ^a   Pen, Ally ^a   Ear, Po Hien ^a   Falgueyret, Jean Pierre ^a   LeRiche, Tammy G ^a   Mancini, Joseph A ^a   Riendeau, Denis ^a   Percival, M David ^a  

^a MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROXYAPATITE; POTASSIUM DIHYDROGEN PHOSPHATE; PROSTAGLANDIN E2; RECOMBINANT PROTEIN; SYNTHETASE; TRITON X 100;

ANIMAL CELL; BACTERIUM ISOLATION; BACULOVIRUS; CONFERENCE PAPER; ENZYME ACTIVITY; GEL FILTRATION; IMMUNOBLOTTING; INSECT CELL; KINETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY;

ANIMALIA; BACTERIA (MICROORGANISMS); INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0038205656     PISSN: 00652598     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4419-9194-2_22     Document Type: Conference Paper

References (6)

1. 2 Synthase that Acts in Concert with Cyclooxygenase-2. J Biol Chem 2000; 275:32783-32792.
2. 2 Biosynthesis. J Biol Chem 2000; 275:32775-32782.
3. Tanikawa, N, Ohmiya Y, Ohkubo H, Hashimoto K, Kangawa K, Kojima M, Ito S, Watanabe K. Identification and Characterization of a Novel Type of Membrane-Associated Prostaglandin E Synthase. Biochem Biophys Res Com 2002; 291:884-889.
4. Ogino N, Miyamoto T, Yamamoto S, Hayaishi O. Prostaglandin Endoperoxide E Isomerase from Bovine Vesicular Gland Microsomes, a Glutathione-requiring Enzyme. J Biol Chem 1977; 252:890-895.
5. Ouellet M, Falgueyret J-P, Ear P-H, Pen A, Mancini JA, Riendeau D, Percival MD. Purification and Characterization of recombinant microsomal prostaglandin E synthase-1. Prot Exp Purif 2002; 26: 489-495.
6. Morgenstern R, Guthenberg C, Depierre JW. Microsomal Glutathione S-Transferase. Purification, Initial Characterization and Demonstration that it is not Identical to the Cytosolic Glutathione S-Transferase A, B and C. Eur J Biochem 1982; 128: 243-248.