Expression of Na,K-ATPase in P. pastoris: Fe2+-catalyzed cleavage of the recombinant enzyme

Annals of the New York Academy of Sciences

Volumn 986, Issue , 2003, Pages 247-248

  Strugatsky, David ^a   Goldshleger, Rivka ^a   Bibi, Eitan ^a   Karlish, Steven J D ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Fe catalyzed cleavage; P. pastoris; Recombinant Na,K ATPase

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ADENOSINE TRIPHOSPHATASE (POTASSIUM); IRON; RECOMBINANT ENZYME;

AMINO ACID SUBSTITUTION; BINDING AFFINITY; CATALYSIS; CELL GROWTH; CHEMICAL BINDING; CONFERENCE PAPER; CONFORMATION; ENZYME ACTIVITY; NONHUMAN; PICHIA PASTORIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION;

PICHIA; PICHIA PASTORIS;

EID: 0038184142     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.2003.tb07173.x     Document Type: Conference Paper

References (4)

1. GOLDSHLEGER, R. & S.J.D. KARLISH. 1997. Fe-catalyzed cleavage of the alpha subunit of Na/K-ATPase: evidence for conformation-sensitive interactions between cytoplasmic domains. Proc. Natl. Acad. Sci. USA 94(18): 9596-9601.
2. PATCHORNIK, G., R. GOLDSHLEGER & S.J.D. KARLISH. 2000. The complex ATP-Fe(2+) serves as a specific affinity cleavage reagent in ATP-Mg(2+) sites of Na,K-ATPase: altered ligation of Fe(2+) (Mg(2+)) ions accompanies the E(1)→E(2) conformational change. Proc. Natl. Acad. Sci. USA 97(22): 11954-11959.
3. PEDERSEN, P.A., J.H. RASMUSSEN & P.L. JORGENSEN. 1996. Consequences of mutations to the phosphorylation site of the alpha-subunit of Na,K-ATPase for ATP binding and E1-E2 conformational equilibrium. Biochemistry 35: 16085-16093.
4. PEDERSEN, P.A., J.R. JORGENSEN & P.L. JORGENSEN. 2001. Importance of conserved α-subunit segment 709GDGVND for Mg2+ binding, phosphorylation, and energy transduction in Na,K-ATPase. J. Biol. Chem. 275: 37588-37595.