Microsupport with two-dimensional geometry (2D-MS) 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHex® (CryoHex)

Cytotechnology

Volumn 39, Issue 3, 2002, Pages 163-170

  Kenda Ropson, N ^a   Lenglois, S ^a   Miller, A O A ^a  

^a 4C Biotech SA   (Belgium)

Author keywords

Anchorage dependent cells; Cryoresponsive; Microhex ; N isopropylacrylamide

Indexed keywords

ANCHORAGE INDEPENDENT GROWTH; ANIMAL CELL; ARTICLE; BIOTECHNOLOGICAL PROCEDURES; CELL CULTURE EQUIPMENT; CELL KINETICS; CELL VIABILITY; CELL VOLUME; CHO CELL; COLD SENSITIVITY; ELECTRON BEAM; GEOMETRY; GROWTH RATE; IRRADIATION; LOW TEMPERATURE PROCEDURES; MASS SPECTROMETRY; NONHUMAN; TEMPERATURE MEASUREMENT; TISSUE CULTURE;

ANIMALIA;

EID: 0038178986     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1023949716842     Document Type: Article

References (13)

1. Brown P.C., Costello M.A.C., Oakley R. and Lewis J.L. 1985. Applications of the mass culturing technique (MCT) in the large scale growth of mammalian cells. In: Feder J. and Tolbert W.R. (eds), Large-Scale Mammalian Cell Culture. Academic Press Inc., New York, pp. 59-71.
2. Chen G., Imanishi Y. and Ito Y. 1998. Effect of protein and cell behaviour on pattern-grafted thermoresponsive polymer. J Biomed Mater Res 42: 38-44.
3. Dormont D. 1999. Transmissible spongiform encephalopathy agents and animal sera. Dev Biol Standard 99: 25-34.
4. Gatot C., Trampler F., Wanderpepen M.-P., Harfield J., Oudshoorn A., Johansson A. et al. 1998. Use of a new microcarrier with two-dimensional geometry for the culture of anchorage-dependent cells in sonoperfused, continuously stirred tank reactors. In: Merten O.-W., Perrin P. and Griffiths B. (eds), New Developments and New Applications in Animal Cell Technology. Kluwer Academic Publishers, Dordrecht, pp. 377-380.
5. Kenda-Ropson N., Mention D., Motte V., Genlain M. and Miller A.O.A. 2001. Microsupport with two-dimensional geometry (2D-MS) 3. In situ determination of the growth kinetics of anchorage-dependent cells by Laser Diffraction Particle Sizing (LDPS). Cytotechnology 37: 49-53.
6. Kikuchi A., Okuhara M., Karikusa F., Sakurai Y. and Okano T. 1998. Two-dimensional manipulation of confluently cultured vascular endothelial cells using temperature-responsive poly(N-' isopropylacrylamide)-grafted surfaces. J Biomater Sci Polymer Edn 9: 1331-1348.
7. Kushida A., Yamato M., Konno C., Kikuchi A., Sakurai Y. and Okano T. 2000. Temperature-responsive culture dishes allow nonenzymatic harvest of differentiated Madin-Darby canine Kidney (MDCK) cell sheets. J Biomed Mater Res 51: 216-223.
8. Kushida A., Yamato M., Konno C., Kikuchi A., Sakurai Y. and Okano T. 1999. Decrease in culture temperature releases monolayer endothelial cells together with deposited fibronectin matrix from temperature-responsive culture surfaces. J Biomed Mater Res 45: 355-362.
9. Kwon O.H., Kikuchi A., Yamato M., Sakurai Y. and Okano T. 2000. Rapid cell sheet detachment from poly (N-isopropylacrylamide)-grafted porous cell culture membranes. J Biomed Mater Res 50: 82-89.
10. Okano T., Yamada M., Karikusa F. and Sakurai Y. 1995. Mechanism of cell detachment from temperature-modulated, hydrophylic-hydrophobic polymer surfaces. Biomaterials 16: 297-303.
11. Van Wezel A.L. 1985. Monolayer growth systems: homogeneous unit processes. In: Spier R.E. and Griffiths B. (eds), Animal Cell Biotechnology Vol. 1. Academic Press Inc., London, pp. 265-282.
12. Yamato M., Okuhara M., Karikusa F., Kikuchi A., Sakurai Y. and Okano T. 1999. Signal transduction and cytoskeletal reorganisation were required for cell detachment from cell culture surfaces grafted with a temperature-responsive polymer. J Biomed Mater Res 44: 44-52.
13. Zecchini T.A. and Smith R.J. 1998. Production of high titre disabled infectious single cycle (DISC) HSV-2 from a microcarrier culture. In: Merten O.-W., Perrin P. and Griffiths B. (eds), New Developments and New Applications in Animal Cell Technology. Kluwer Academic Publishers, Dordrecht, pp. 569-571.