pckA-deficient Mycobacterium bovis BCG shows attenuated virulence in mice and in macrophages

Microbiology

Volumn 149, Issue 7, 2003, Pages 1829-1835

  Liu, Keyi ^a   Yu, Jinzhi ^a   Russell, David G ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID; CITRIC ACID; GLUCOSE; GREEN FLUORESCENT PROTEIN; OXALOACETIC ACID; PALMITIC ACID; PHOSPHOENOLPYRUVATE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (PYROPHOSPHATE);

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ATTENUATION; BACTERIAL STRAIN; BACTERIAL SURVIVAL; BACTERIAL VIRULENCE; CATALYSIS; CITRIC ACID CYCLE; CONTROLLED STUDY; DECARBOXYLATION; DOWN REGULATION; ENZYME ACTIVITY; ENZYME DEFICIENCY; FEMALE; GENE CONTROL; GENE DELETION; GLUCONEOGENESIS; GRANULOMA; MACROPHAGE; MICROBIAL ATTENUATION; MOUSE; MYCOBACTERIOSIS; MYCOBACTERIUM BOVIS; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; UPREGULATION;

ACTINOBACTERIA (CLASS); ANIMALIA; BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM BOVIS BCG; UNCULTURED ACTINOMYCETE;

EID: 0038148638     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.26234-0     Document Type: Article

References (13)

1. Barbieri, J. T., Austin, F. E. & Cox, C. D. (1981). Distribution of glucose incorporated into macromolecular material by Treponema pallidum. Infect Immun 31, 1071-1077.
2. Collins, D. M., Wilson, T., Campbell, S., Buddle, B. M., Wards, B. J., Hotter, G. & de Lisle, G. W. (2002). Production of avirulent mutants of Mycobacterium bovis with vaccine properties by the use of illegitimate recombination and screening of stationary-phase cultures. Microbiology 148, 3019-3027.
3. Cymeryng, C., Cazzulo, J. J. & Cannata, J. J. (1995). Phosphoenolpyruvate carboxykinase from Trypanosoma cruzi. Purification and physicochemical and kinetic properties. Mol Biochem Parasitol 73, 91-101.
4. Hou, S. Y., Chao, Y. P. & Liao, J. C. (1995). A mutant phosphoenolpyruvate carboxykinase in Escherichia coli conferring oxaloacetate decarboxylase activity. J Bacteriol 177, 1620-1623.
5. Liu, K., Zinker, S., Arguello, C. & Salgado, L. M. (2000). Isolation and analysis of a new developmentally regulated gene from amastigotes of Leishmania mexicana mexicana. Parasitol Res 86, 140-150.
6. McKinney, J. D., Honer zu Bentrup, K., Munoz-Elias, E. J. & 7 other authors (2000). Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase. Nature 406, 735-738.
7. Mukhopadhyay, B., Concar, E. M. & Wolfe, R. S. (2001). A GTP-dependent vertebrate-type phosphoenolpyruvate carboxykinase from Mycobacterium smegmatis. J Biol Chem 276, 16137-16145.
8. Osteras, M., Finan, T. M. & Stanley, J. (1991). Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype. Mol Gen Genet 230, 257-269.
9. Pelicic, V., Reyrat, J. M. & Gicquel, B. (1996). Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors. Mol Microbiol 20, 919-925.
10. Rohrer, S. P., Saz, H. J. & Nowak, T. (1986). Purification and characterization of phosphoenolpyruvate carboxykinase from the parasitic helminth Ascaris suum. J Biol Chem 261, 13049-13055.
11. Schocke, L. & Weimer, P. J. (1997). Purification and characterization of phosphoenolpyruvate carboxykinase from the anaerobic ruminal bacterium Ruminococcus flavefaciens. Arch Microbiol 167, 289-294.
12. Scovill, W. H., Schreier, H. J. & Bayles, K. W. (1996). Identification and characterization of the pckA gene from Staphylococcus aureus. J Bacteriol 178, 3362-3364.
13. Smith, C. V., Huang, C. C., Miczak, A., Russell, D. G., Sacchettini, J. C. & Honer zu Bentrup, K. (2003). Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis. J Biol Chem 278, 1735-1743.