A novel fluorogenic substrate for horseradish peroxidase : Efficient detection of membrane-bound nucleic acids and simultaneous detection of DNAs

Chemistry Letters

Volumn , Issue 10, 1997, Pages 1075-1076

  Fujita, Satoshi ^a   Toru, Takeshi ^a   Kondoh, Yasumitu ^b   Kagiyama, Naoto ^b   Momiyama, Masayoshi ^b  

^a NAGOYA INSTITUTE OF TECHNOLOGY   (Japan)

^b Aisin Cosmos R and D Co   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0038133500     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.1997.1075     Document Type: Article

References (17)

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2. For example, see J. Ju, I. Kheterpal, J. R. Scherer, C. Ruman, C. W. Fuller, A. N. Glazer, and R. A. Mathies, Anal. Biochem., 231, 131 (1995) and references cited therein.
3. Y. X. Ci, Y. Z. Li, and X. J. Liu, Anal. Chem., 67, 1785 (1995).
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6. a) S. Fujita, N. Kagiyama, M. Momiyama, and T. Toru, Chem. Lett., 1996, 1073;
7. b) S. Fujita, T. Toru, Y. Kitagawa, N. Kagiyama, and M. Momiyama, Anal. Chim. Acta, 339, 289 (1997).
8. S. Beck, T. O'Keeffe, J. M., Coull, and H. Koster, Nucleic Acids Res., 17, 5115 (1989); G. An, G. Luo, R. W. Veltri, and S. M. O'Hara, Biotechniques, 20, 342 (1996).
9. S. Beck, T. O'Keeffe, J. M., Coull, and H. Koster, Nucleic Acids Res., 17, 5115 (1989); G. An, G. Luo, R. W. Veltri, and S. M. O'Hara, Biotechniques, 20, 342 (1996).
10. S. Fujita, T. Toru, Y. Kondoh, M. Momiyama, N. Kagiyama, and S. H. Hori, Acta Histochem. Cytochem., 30, 165 (1997).
11. Fluorometric detection was carried out with a fluorescence detector Epilight-UV-FA-1100 (Aisin Cosmos R & D).
12. 2, see; P. D. Josephy, T. E. Eling, and R. P. Mason, J. Biol. Chem., 258, 5561 (1983).
13. The spot test was carried out as described previously, see ref. 6b.
14. The operation transferring the target DNA to membrane and hybridizing the probe to target DNA may bring about a reduction of the sensitivity of Southern blot hybridization in comparison with the results in the spot test. Other known methods are much less sensitive than our method; ALP-BCIP/NBT; detection limit, ca, 5pg pBR 322 DNA (size 2kb); see J. D. Norton, J. Connor, and R. J. Avery, Nucleic Acids Res., 12, 3445 (1984). POD-Luminol method; detection limit, ca, 5pg; see C. Kessler, Nonisotopic DNA Probe Techniques, Academic Press (1992).
15. The operation transferring the target DNA to membrane and hybridizing the probe to target DNA may bring about a reduction of the sensitivity of Southern blot hybridization in comparison with the results in the spot test. Other known methods are much less sensitive than our method; ALP-BCIP/NBT; detection limit, ca, 5pg pBR 322 DNA (size 2kb); see J. D. Norton, J. Connor, and R. J. Avery, Nucleic Acids Res., 12, 3445 (1984). POD-Luminol method; detection limit, ca, 5pg; see C. Kessler, Nonisotopic DNA Probe Techniques, Academic Press (1992).
16. The procedure for the southern blot hybridization is essentially the same as described previously, see ref. 6b.
17. 2. After incubation for 2h, the membrane was washed with distilled water and dried in the air. Orange and bluish-green signals appeared under irradiation of ultraviolet light (312 nm) and were recorded with a 35 nun color film (Kodak) using a cut filter (400 nm).