cDNA cloning and high-level expression of a thrombin-like enzyme from Agkistrodon acutus venom

Methods and Findings in Experimental and Clinical Pharmacology

Volumn 25, Issue 4, 2003, Pages 253-257

  Zha, X D ^a,b   Ren, B ^a   Liu, J ^a   Xu, K S ^c  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b ANHUI UNIVERSITY   (China)

^c NATIONAL INSTITUTE FOR THE CONTROL OF PHARMACEUTICAL AND BIOLOGICAL PRODUCTS   (China)

Author keywords

Accelerated evolution; Agkistrodon acutus; Recombinant expression; Thrombin like enzyme

Indexed keywords

ACUTIN; ARGININE ESTERASE; CELL PROTEIN; COMPLEMENTARY DNA; FIBRINOGEN; HYBRID PROTEIN; NATURAL PRODUCT; RECOMBINANT ENZYME; SNAKE VENOM; THROMBIN LIKE ENZYME; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AGKISTRODON ACUTUS; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLEEDING; CAPILLARY ZONE ELECTROPHORESIS; CELL INCLUSION; CHINA; CONTROLLED STUDY; DRUG INDUSTRY; ENZYME ACTIVITY; GENE AMPLIFICATION; GEOGRAPHIC DISTRIBUTION; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SNAKE; SPECIES IDENTIFICATION; STRUCTURE ACTIVITY RELATION; SYMPTOMATOLOGY;

EID: 0038125745     PISSN: 03790355     EISSN: None     Source Type: Journal    
DOI: 10.1358/mf.2003.25.4.769672     Document Type: Article

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