Isolation of a virulent Piscirickettsia salmonis from the brain of naturally infected coho salmon

Bulletin of the European Association of Fish Pathologists

Volumn 20, Issue 6, 2000, Pages 261-264

  Skarmeta, A M ^a   Henriquez V ^a   Zahr, M ^a,b   Orrego, C ^a,c   Marshall, S H ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

^b UNIVERSIDAD DE VALPARAÍSO   (Chile)

^c SAN FRANCISCO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0038102451     PISSN: 01080288     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Almendras, F. and Fuentealba, C., (1997). Salmonid rickettsial septicemia caused by Piscirickettsia salmonis: a review. Dis. Aquat. Org. 29: 137-144.
2. Fryer, J.L., Lannan, C., Giovannoni, S.J. and Wood, N.D. (1992) Piscirickettsia salmonis gen. nov. sp.nov., the causative agent of an epizootic disease in salmonid fishes. Int. J. Syst. Bacteriol. 42: 120-126.
3. Grant, A.N., Brown, A.G., Cox, D.J., Birkbeck, T.H. and Griffen, A.A. (1996) Rickettsia-like organism in farmed salmon. Vet. Rec. 138: 423-424.
4. Heath, S., Pak, S., Marshall, S., Prager, E.M. and Orrego, C. (2000) Monitoring Piscirickettsia salmonis by denaturant gel electrophoresis and competitive PCR. Dis. Aquat. Org. 41: 19-29.
5. House, M.L., Bartholomew, J.L., Winton, J.R. and Fryer, J.L. (1999) Relative virulence of three isolates of Piscirickettsia salmonis for coho salmon Oncorhynchus kisutch. Dis. Aquat. Org. 35:107-113.
6. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
7. Marshall, S., Heath, S., Henríquez, V. and Orrego, C. (1998) Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via PCR. Appl. Environ. Microbiol. 64: 3066-3069.
8. Mauel, M. J., Giovannoni, S.J. and Fryer, J.L. (1999) Phylogenetic analysis of Piscirickettsia salmonis by 16S, internal transcribed spacer (ITS) and 23S ribosomal DNA sequencing. Dis. Aquat. Org. 35:115-123.
9. Reed, L.J. and Muench, H. (1938) A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27: 493-497.
10. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Nat. Acad. Sci. USA 76: 4350-4354.
11. Office International des Epizooties, OIE. (1997). Diagnostic manual for aquatic animal diseases. Paris, France, pp. 161-168.