RNA polymerase β mutations have reduced σ70 synthesis leading to a hyper-temperature-sensitive phenotype of a σ70 mutant

Journal of Bacteriology

Volumn 179, Issue 13, 1997, Pages 4292-4298

  Zhou, Yan Ning ^a   Jin, Ding Jun ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; HEAT SHOCK PROTEIN; RNA POLYMERASE;

ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SYNTHESIS; RNA SYNTHESIS; TEMPERATURE SENSITIVE MUTANT; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0038070724     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.13.4292-4298.1997     Document Type: Article

References (32)

1. Ang, D., and C. Georgopoulos. 1989. The heat-shock-regulated grpE gene of Escherichia coli is required for bacterial growth at all temperatures but is dispensable in certain mutant backgrounds. J. Bacteriol. 171:2748-2755.
2. Baker, T. A., A. D. Grossman, and C. Gross. 1984. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81:6779-6783.
3. Berk, A. J., and P. A. Sharp. 1977. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 nuclease-digested hybrids. Cell 12:721-732.
4. Bukau, B., and G. C. Walker. 1989. Cellular defects caused by deletion of the Escherichia coli dnaK gene indicate roles for heat shock protein in normal metabolism. J. Bacteriol. 171:2337-2346.
5. Burton, Z. F., C. A. Gross, K. K. Watanabe, and R. R. Burgess. 1983. The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12. Cell 32:335-349.
6. Cashel, M., D. R. Gentry, V. J. Hernandez, and D. Vinella. 1996. The stringent response, p. 1458-1496. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
7. Choy, H., and S. Adhya. 1992. Control of gal transcription through DNA looping: inhibition of the initial transcribing complex. Proc. Natl. Acad. Sci. USA 89:11264-11268.
8. Gross, C. A., A. D. Grossman, H. Liebke, W. Walter, and R. R. Burgess. 1984. Effects of the mutant sigma allele rpoD800 on the synthesis of specific macromolecular components of the Escherichia coli K12 cell. J. Mol. Biol. 172:280-300.
9. Grossman, A. D., R. R. Burgess, W. Walter, and C. A. Gross. 1983. Mutations in the lon gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase. Cell 32:151-159.
10. Grossman, A. D., W. E. Taylor, Z. F. Burton, R. R. Burgess, and C. A. Gross. 1985. Stringent response in Escherichia coli induces expression of heat shock proteins. J. Mol. Biol. 186:357-365.
11. Hager, D. A., D. J. Jin, and R. R. Burgess. 1990. Use of mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase. Biochemistry 29:7890-7894.
12. Harris, J. D., J. S. Heilig, I. I. Martinez, R. Calendar, and L. A. Isaksson. 1978. A temperature-sensitive E. coli mutant affecting the sigma subunit of DNA-dependent RNA polymerase. Proc. Natl. Acad. Sci. USA 75:6177-6181.
13. Hernandez, V. J., and M. Cashel. 1995. Changes in conserved region 3 of sigma 70 mediate ppGpp-dependent function in vivo. J. Mol. Biol. 252:536-549.
14. Hu, J. C., and C. A. Gross. 1983. Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor. Mol. Gen. Genet. 191:492-498.
15. Hu, J. C., and C. A. Gross. 1985. Mutations in the sigma subunit of E. coli RNA polymerase which affect positive control of transcription. Mol. Gen. Genet. 199:7-13.
16. Jin, D. J., and C. A. Gross. 1988. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that led to rifampicin resistance. J. Mol. Biol. 202:45-58.
17. Jin, D. J., and C. A. Gross. 1989. Characterization of the pleiotropic phenotypes of rifampin-resistant rpoB mutants of Escherichia coli. J. Bacteriol. 171:5229-5231.
18. Jin, D. J., and C. L. Turnbough, Jr. 1994. An Escherichia coli RNA polymerase defective in transcription due to its overproduction of abortive initiation products. J. Mol. Biol. 236:72-80.
19. 18a. Jin, D. J., and Y. N. Zhou. 1996. Mutational analysis of structure-function relationship of RNA polymerase in Escherichia coli. Methods Enzymol. 273:300-319.
20. Kusukawa, N., and T. Yura. 1988. Heat shock protein GroE of Escherichia coli: key protective roles against thermal stress. Genes Dev. 2:874-882.
21. Liebke, H., C. Gross, W. Walter, and R. Burgess. 1980. A new mutation rpoD800, affecting the sigma subunit of E. coli RNA polymerase is allelic to two other sigma mutants. Mol. Gen. Genet. 177:277-282.
22. Lowe, P. A., U. Aebi, C. Gross, and R. R. Burgess. 1981. In vitro thermal inactivation of a temperature-sensitive a subunit mutant (rpoD800) of Escherichia coli RNA polymerase proceeds by aggregation. J. Biol. Chem. 256: 2010-2015.
23. Lupski, J. R., B. L. Smiley, and G. N. Godson. 1983. Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12. Mol. Gen. Genet. 189:48-57.
24. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. 23a. Murphy, H., D. J. Jin, and M. Cashel. Unpublished results.
26. Osawa, T., and T. Yura. 1981. Effects of reduced amount of RNA polymerase sigma factor on gene expression and growth of Escherichia coli: studies of the rpoD40 (amber) mutation. Mol. Gen. Genet. 184:166-173.
27. Taylor, W. E., D. B. Straus, A. D. Grossman, Z. F. Burton, C. A. Gross, and R. R. Burgess. 1984. Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase. Cell 38:371-381.
28. Xiao, H., M. Kalman, K. Ikehara, S. Zemel, G. Glaser, and M. Cashel. 1991. Residual guanosine 3′,5′-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations. J. Biol. Chem. 266:5980-5990.
29. 32. J. Bacteriol. 170:3640-3649.
30. 32 with a small deletion in conserved region 3 of σ has reduced affinity for core RNA polymerase. J. Bacteriol. 174:5005-5012.
31. 32. J. Bacteriol. 174:7128-7137.
32. Zhou, Y. N., and D. J. Jin. Unpublished data.