Identification, cDNA cloning, expression, crystallization and preliminary X-ray analysis of an exceptionally halotolerant carbonic anhydrase from Dunaliella salina

Acta Crystallographica - Section D Biological Crystallography

Volumn 59, Issue 6, 2003, Pages 1084-1086

  Premkumar, Lakshmanane ^a   Greenblatt, Harry M ^a   Bageshwar, Umesh K ^a,b   Savchenko, Tatyana ^a   Gokhman, Irena ^a   Zamir, Ada ^a   Sussman, Joel L ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGAE, GREEN; AMINO ACID SEQUENCE; CARBONIC ANHYDRASES; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA, COMPLEMENTARY; MOLECULAR SEQUENCE DATA; PLASMIDS; X-RAY DIFFRACTION;

ALGAE; DUNALIELLA; DUNALIELLA SALINA; ESCHERICHIA COLI; EUKARYOTA; SALINA;

EID: 0038044745     PISSN: 09074449     EISSN: None     Source Type: Journal    
DOI: 10.1107/S0907444903007066     Document Type: Article

References (11)

1. Avron, M. (1986). Trends Biochem. 11, 5-6.
2. Fisher, M., Gokhman, I., Pick, U. & Zamir, A. (1996). J. Biol. Chem. 271, 17718-17723.
3. Fisher, M., Pick, U. & Zamir, A. (1994). Plant Physiol. 106, 1359-1365.
4. Gokhman, I., Fisher, M., Pick, U. & Zamir, A. (1999). Microbiology and Biogeochemistry of Hypersaline Environments, edited by A. Oren, pp. 203-213. Boca Raton, FL, USA: CRC Press.
5. Madern, D., Ebel, C. & Zaccai, G. (2000). Extremophiles, 4, 91-98.
6. Mevarech, M., Frolow, F. & Gloss, L. M. (2000). Biophys. Chem. 86, 155-164.
7. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
8. Premkumar, L., Bageshwar, U., Gokhman, I., Zamir, A. & Sussman, J. L. (2003). Protein. Expr. Purif. 28, 151-157.
9. Sadka, A., Lers, A., Zamir, A. & Avron, M. (1989). FEBS Lett. 244, 93-98.
10. Stams, T. & Christianson, D. W. (2000). EXS, pp. 159-174.
11. Whitney, P. (1974). Anal. Biochem. 57, 467-476.