The C-terminal flexible domain of the heme chaperone CcmE is important but not essential for its function

Journal of Bacteriology

Volumn 185, Issue 13, 2003, Pages 3821-3827

  Enggist, Elisabeth ^a   Thöny Meyer, Linda ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; CYTOCHROME C; HEME DERIVATIVE; HISTIDINE; PROTEIN CCME; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; APOENZYMES; BACTERIAL OUTER MEMBRANE PROTEINS; CELL MEMBRANE; CYTOCHROME C GROUP; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; HEME; HEMEPROTEINS; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; MUTATION; PERIPLASM; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SPECTRUM ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0038039207     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.13.3821-3827.2003     Document Type: Article

References (21)

1. Arnesano, F., I. Banci, P. D. Barker, I. Bertini, A. Rosato, X. C. Su, and M. S. Viezzoli. 2002. Solution structure and characterization of the heme chaperone CcmE. Biochemistry 41:13587-13594.
2. Daltrop, O., J. M. Stevens, C. W. Higham, and S. J. Ferguson. 2002. The CcmE protein of the type c cytochrome biogenesis system: unusual in vitro heme incorporation into apo-CcmE and transfer from holo-CcmE to apocytochrome. Proc. Natl. Acad. Sci. USA 99:9703-9708.
3. Enggist, E., M. J. Schneider, H. Schulz, and L. Thöny-Meyer. 2003. Biochemical and mutational characterization of the heme chaperone CcmE reveals a heme binding site. J. Bacteriol. 185:175-183.
4. Enggist, E., L. Thöny-Meyer, P. Güntert, and K. Pervushin. 2002. NMR structure of the heme chaperone CcmE reveals a novel functional motif. Structure 10:1551-1557.
5. Fabianek, R. A., T. Hofer, and L. Thöny-Meyer. 1999. Characterization of the Escherichia coli CcmH protein reveals new insights into the redox pathway required for cytochrome c maturation. Arch. Microbiol. 171:92-100.
6. Fuhrhop, J.-H., and K. M. Smith (ed.). 1975. Porphyrins and metalloporphyrins, p. 757-868. Elsevier, Amsterdam, The Netherlands.
7. Grove, J., S. Tanapongpipat, G. Thomas, L. Griffiths, H. Crooke, and J. Cole. 1996. Escherichia coli K-12 genes essential for the synthesis of type c cytochromes and a third nitrate reductase located in the periplasm. Mol. Microbiol. 18:467-481.
8. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-563.
9. Iobbi-Nivol, C., H. Crooke, L. Griffith, J. Grove, H. Hussain, J. Pommier, V. Méjean, and J. A. Cole. 1994. A reassessment of the range of type c cytochromes synthesized by Escherichia coli K-12. FEMS Microbiol. Lett. 119:89-94.
10. Murzin, A. G. 1993. OB (oligonucleotide/oligosaccharide binding)-fold: common structural and functional solution for non-homologous sequences. EMBO J. 12:861-867.
11. Potter, L. C., and J. A. Cole. 1999. Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12. Biochem. J. 344:69-76.
12. Reid, E., D. J. Eaves, and J. A. Cole. 1998. The CcmE protein from Escherichia coli is a haem-binding protein. FEMS Microbiol. Lett. 166:369-375.
13. Ren, Q., U. Ahuja, and L. Thöny-Meyer. 2002. A bacterial cytochrome c heme lyase: CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome c. J. Biol. Chem. 277:7657-7663.
14. Ren, Q., and L. Thöny-Meyer. 2001. Physical interaction of CcmC with heme and the heme chaperone CcmE during cytochrome c maturation. J. Biol. Chem. 276:32591-32596.
15. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1998. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
16. Schulz, H., R. A. Fabianek, E. C. Pellicioll, H. Hennecke, and L. Thöny-Meyer. 1999. Heme transfer to the heme chaperone CcmE during cytohrome c maturation requires the CcmC protein, which may function independently of the ABC-transporter CcmAB. Proc. Natl. Acad. Sci. USA 96:6462-6467.
17. Schulz, H., H. Hennecke, and L. Thöny-Meyer. 1998. Prototype of a heme chaperone essential for cytochrome c maturation. Science 281:1197-1200.
18. Schulz, H., E. Pellicioll, and L. Thöny-Meyer. 2000. New insights into the role of CcmC, CcmD, and CcmE in the haem delivery pathway during cytochrome c maturation by a complete mutational analysis of the conserved tryptophan-rich motif of CcmC. Mol. Microbiol. 37:1379-1388.
19. Tanapongpipat, S., E. Reid, J. A. Cole, and H. Crooke. 1998. Transcriptional control and essential roles of Escherichia coli ccm gene products in formate-dependent nitrite reduction and cytochrome c synthesis. Biochem. J. 334:355-365.
20. Thöny-Meyer, L., F. Fischer, P. Künzler, D. Ritz, and H. Hennecke. 1995. Escherichia coli genes required for cytochrome c maturation. J. Bacteriol. 177:4321-4326.
21. 550 in Eseherichia coli. Eur. J. Biochem. 235:754-761.