Changes in cytoskeletal and tight junctional proteins correlate with decreased permeability induced by dexamethasone in cultured rat brain endothelial cells

Neuroscience Letters

Volumn 344, Issue 2, 2003, Pages 112-116

  Romero, Ignacio A ^a,b,d   Radewicz, Katrina ^a   Jubin, Emmanuelle ^b   Michel, C Charles ^c   Greenwood, John ^a   Couraud, Pierre Olivier ^b   Adamson, Peter ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b INSTITUT COCHIN   (France)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d OPEN UNIVERSITY   (United Kingdom)

Author keywords

Blood brain barrier; Brain endothelium; Cell junctions; Dexamethasone; Glucocorticoids; Permeability

Indexed keywords

ACTIN; BETA CATENIN; CORTACTIN; CYTOSKELETON PROTEIN; DEXAMETHASONE; DEXTRAN; FLUORESCEIN; GLUCOCORTICOID; OCCLUDIN; PROTEIN; PROTEIN ADHEREN; PROTEIN ZO1; SUCROSE; UNCLASSIFIED DRUG;

ANIMAL CELL; BLOOD BRAIN BARRIER; BRAIN CELL; CELL ASSAY; CELL CULTURE; CELL IMMORTALIZATION; CELL LINE; CELL MEMBRANE PERMEABILITY; CELL STRUCTURE; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; CYTOSKELETON; ENDOTHELIUM CELL; HORMONE ACTION; HORMONE DETERMINATION; MOLECULAR SIZE; NONHUMAN; PHENOTYPE; POROSITY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; REVIEW; TIGHT JUNCTION;

EID: 0038018620     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(03)00348-3     Document Type: Review

References (20)

1. Antonetti D.A., Wolpert E.B., DeMaio L., Harhaj N.S., Scaduto R.C. Jr. Hydrocortisone decreases retinal endothelial cell water and solute flux coincident with increased content and decreased phosphorylation of occludin. J. Neurochem. 80:2002;667-677.
2. Bates D.O., Hillman N.J., Williams B., Neal C.R., Pocock T.M. Regulation of microvascular permeability by vascular endothelial growth factors. J. Anat. 200:2002;581-597.
3. Furuse M., Fujita K., Hiiragi T., Fujimoto K., Tsukita S. Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J. Cell Biol. 141:1998;1539-1550.
4. Gille J., Reisinger K., Westphal-Varghese B., Kaufmann R. Decreased mRNA stability as a mechanism of glucocorticoid-mediated inhibition of vascular endothelial growth factor gene expression by cultured keratinocytes. J. Invest. Dermatol. 117:2001;1581-1587.
5. Gumbleton M., Audus K.L. Progress and limitations in the use of in vitro cell cultures to serve as a permeability screen for the blood-brain barrier. J. Pharm. Sci. 90:2001;1681-1698.
6. Harkness K.A., Adamson P., Sussman J.D., Davies-Jones G.A., Greenwood J., Woodroofe M.N. Dexamethasone regulation of matrix metalloproteinase expression in CNS vascular endothelium. Brain. 123:(Pt 4):2000;698-709.
7. Hirase T., Staddon J.M., Saitou M., Ando-Akatsuka Y., Itoh M., Furuse M., Fujimoto K., Tsukita S., Rubin L.L. Occludin as a possible determinant of tight junction permeability in endothelial cells. J. Cell. Sci. 110:(Pt 14):1997;1603-1613.
8. Hoheisel D., Nitz T., Franke H., Wegener J., Hakvoort A., Tilling T., Galla H.J. Hydrocortisone reinforces the blood-brain properties in a serum free cell culture system. Biochem. Biophys. Res. Commun. 247:1998;312-315.
9. Nauck M., Karakiulakis G., Perruchoud A.P., Papakonstantinou B., Roth M. Corticosteroids inhibit the expression of the vascular endothelial growth factor gene in human vascular smooth muscle cells. Eur. J. Pharmacol. 341:1998;309-315.
10. Regina A., Romero I.A., Greenwood J., Adamson P., Bourre J.M., Couraud P.O., Roux E. Dexamethasone regulation of P-glycoprotein activity in an immortalized rat brain endothelial cell line, GPNT. J. Neurochem. 73:1999;1954-1963.
11. Renkin E.M. Capillary transport of macromolecules: pores and other endothelial pathways. J. Appl. Physiol. 58:1985;315-325.
12. Romero I.A., Prevost M.C., Perret E., Adamson P., Greenwood J., Couraud R.O., Ozden S. Interactions between brain endothelial cells and human T-cell leukemia virus type 1-infected lymphocytes: mechanisms of viral entry into the central nervous system. J. Virol. 74:2000;6021-6030.
13. Rubin L.L., Hall D.E., Porter S., Barbu K., Cannon C., Horner H.C., Janatpour M., Liaw C.W., Manning K., Morales J., Tanner L.I., Tomaselli K.J., Bard F. A cell culture model of the blood-brain barrier. J. Cell Biol. 115:1991;1725-1735.
14. Rubin L.L., Staddon J.M. The cell biology of the blood-brain barrier. Annu. Rev. Neurosci. 22:1999;11-28.
15. Saitou M., Fujimoto K., Doi Y., Itoh M., Fujimoto T., Furuse M., Takano H., Noda T., Tsukita S. Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions. J. Cell Biol. 141:1998;397-408.
16. Schulze C., Smales C., Rubin L.L., Staddon J.M. Lysophosphatidic acid increases tight junction permeability in cultured brain endothelial cells. J. Neurochem. 68:1997;991-1000.
17. van Bree J.B., de Boer A.G., Danhof M., Ginsel L.A., Breimer D.D. Characterization of an 'in vitro' blood-brain barrier: effects of molecular size and lipophilicity on cerebrovascular endothelial transport rates of drugs. J. Pharmacol. Exp. Ther. 247:1988;1233-1239.
18. Wong V., Ching D., McCrea P.D., Firestone G.L. Glucocorticoid down-regulation of fascin protein expression is required for the steroid-induced formation of tight junctions and cell-cell interactions in rat mammary epithelial tumor cells. J. Biol. Chem. 274:1999;5443-5453.
19. Woo P.L., Cercek A., Desprez P.Y., Firestone G.L. Involvement of the helix-loop-helix protein Id-1 in the glucocorticoid regulation of tight junctions in mammary epithelial cells. J. Biol. Chem. 275:2000;28649-28658.
20. Zettl K.S., Sjaastad M.D., Riskin P.M., Parry G., Machen T.E., Firestone G.L. Glucocorticoid-induced formation of tight junctions in mouse mammary epithelial cells in vitro. Proc. Natl. Acad. Sci. USA. 89:1992;9069-9073.