Do fluorescent drugs show you more than you wanted to know?

British Journal of Pharmacology

Volumn 139, Issue 2, 2003, Pages 187-189

  McGrath, J C ^a   Daly, C J ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

2 Adrenoceptors; BODIPY TMR CGP; CGP 12177; Fluorescent ligands; GPCR; Intracellular receptors; Ligand binding; Partial agonism; Plasmalemmal receptors

Indexed keywords

3 ISOPROPYLAMINO 1 (7 METHYL 4 INDANYLOXY) 2 BUTANOL; 4 (3 TERT BUTYLAMINO 2 HYDROXYPROPOXY) 2 BENZIMIDAZOLONE; ALPHA 1 ADRENERGIC RECEPTOR; ANGIOTENSIN; BENZIMIDAZOLE DERIVATIVE; BETA 1 ADRENERGIC RECEPTOR STIMULATING AGENT; BETA 2 ADRENERGIC RECEPTOR; BETA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; BETA 3 ADRENERGIC RECEPTOR STIMULATING AGENT; BORDIFLUOROPYRROMETHANE TETRAMETHYLRHODAMINE 4 (3 TERT BUTYLAMINO 2 HYDROXYPROPOXY) 2 BENZIMIDAZOLONE; BRADYKININ; CALCIUM; FLUORESCEIN ISOTHIOCYANATE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LIGAND; NEUROPEPTIDE; OPIATE; RADIOLIGAND; TRITIUM; UNCLASSIFIED DRUG;

ALLOSTERISM; BINDING AFFINITY; BLOOD VESSEL; CELL TYPE; CELLULAR DISTRIBUTION; CHEMICAL LABELING; CHO CELL; CYCLIC AMP RESPONSIVE ELEMENT; DRUG CONFORMATION; DRUG DISTRIBUTION; DRUG MECHANISM; DRUG RECEPTOR BINDING; ENERGY TRANSFER; FLUORESCENCE; FLUORESCENCE ANALYSIS; GENETIC TRANSCRIPTION; IMAGING; LIGAND BINDING; LIPOPHILICITY; NERVE; NEUROTRANSMISSION; NOTE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SMOOTH MUSCLE; VASCULAR SMOOTH MUSCLE;

ANIMALS; BORON COMPOUNDS; CYCLOPENTANES; FLUORESCENT DYES; HUMANS; LIGANDS; PYRROLES; RECEPTORS, ADRENERGIC, BETA-2;

EID: 0037930708     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0705288     Document Type: Note

References (26)

1. ARRIBAS, S.M., DOMINICZAK, A.F. & McGRATH, J.C. (1997). Reduced noradrenaline-mediated vasodilatation and β-adrenergic density in the basilar artery during ageing. J. Vasc. Res., 34(S1), 6.
2. ARTTAMANGKUL, S., ALVAREZ-MAUBECIN, V., THOMAS, G., WILLIAMS, J.T. & GRANDY, D.K. (2000). Binding and internalization of fluorescent opioid peptide conjugates in living cells. Mol. Pharmacol., 58, 1570-1580.
3. 2-adrenoceptor. Br. J. Pharmacol., 137, 400-408.
4. 2-adrenoceptor agonist. Br. J. Pharmacol., 139, 232-242.
5. BRAHMADEVARA, N., MacDONALD, A., McGRATH, J.C. & DALY, C.J., (2002). Measurement of cellular and tissue distribution of beta-adrenoceptors in rat thoracic aorta using BODIPY-CGP 12177. Br. J. Pharmacol., 137, 93P.
6. BUKU, A., PRICE, J.A., MENDLOWITZ, M. & MASUR, S. (2001). Mast cell degranulating peptide binds to RBL-2H3 mast cell receptors and inhibits IgE binding. Peptides, 22, 1993-1998.
7. COLEMAN, R.A., JOHNSON, M., NIALS, A.T. & VARDEY, C.J. (1996). Exosites: their current status, and their relevance to the duration of action of long-acting beta 2-adrenoceptor agonists. Trends Pharmacol. Sci., 17, 324-330.
8. 1-adrenoceptors by fluorescent ligand binding and image analysis reveals identical binding properties of clustered and diffuse populations of receptors. J. Pharmacol. Exp. Ther., 286, 984-990.
9. FABRY, M., CABRELE, C., HOCKER, H. & BECK-SICKINGER, A.G. (2000). Differently labeled peptide ligands for rapid investigation of receptor expression on a new human glioblastoma cell line. Peptides, 21, 1885-1893.
10. HEITHIER, H., HALLMANN, D., BOEGE, F., REILANDER, H., DEES, C., JAEGGI, K.A., ARNDT-JOVIN, D., JOVIN, T.M. & HELMREICH, E.J.M. (1994). Synthesis and properties of fluorescent β-adrenoceptor ligands. Biochemistry, 33, 9126-9134.
11. HOWL, J. (1999). Fluorescent and biotinylated probes for B-2 bradykinin receptors: agonists and antagonists. Peptides, 20, 515-518.
12. 2+ transients in varicosities of the mouse vas deferens. Br. J. Pharmacol., 135, 1845-1850.
13. JARAJAPU, Y.P.R., MacDONALD, A., HILLIER, C., McGRATH, J.C., MacKENZIE, J.F. & DALY, C.J. (2002). Quantitative imaging of QAPB-associated fluorescence in smooth muscle cells from human skeletal muscle resistance arteries. Br. J. Pharmacol., 135(Suppl), 303P.
14. JENKINSON, K.M., MORGAN, J.M., FURNESS, J.B. & SOUTHWELL, B.R. (1999). Neurons bearing NK3 tachykinin receptors in the guinea-pig ileum revealed by specific binding of fluorescently labelled agonists. Histochem. Cell Biol., 112, 233-246.
15. 1-adrenoceptor ligand binding sites. J. Pharmacol. Exp. Ther., 294, 434-443.
16. MADSEN, B.W., BEGLAN, C.L. & SPIVAK, C.E. (2000). Fluorescein-labeled naloxone binding to mu opioid receptors on live Chinese hamster ovary cells using confocal fluorescent microscopy. J. Neurosci. Methods, 97, 123-131.
17. 2+ transients in varicosities of the mouse vas deferens: commentary on Jackson and Cunnane. Br. J. Pharmacol., 135, 1841-1843.
18. McGRATH, J.C., ARRIBAS, S.M. & DALY, C.J. (1996). Fluorescent ligands for the study of receptors. Trends Pharmacol. Sci., 17, 393-399.
19. 1-adrenoceptors in native smooth muscle cells. J. Autonom. Pharmacol., 19, 303-310.
20. 1-adrenoceptors in human prostate. Eur. Urol., 36(Suppl 1), 80-88.
21. McGRATH, J.C., PEDIANI, J.D., MacMILLAN, J., MacKENZIE, J., DEIGHAN, C., WOOLHEAD, A., McGRORY, S.P., McBRIDE, M., ALI, Z., MALEKZADEH-SHAFAROUDI, M., COTECCHIA, S., ARRIBAS, S.M., VILA, E., BRIONES, A., PEREZ, D., MULLINS, J., TSUJIMOTO, G. & DALY, C.J. (2002). Adventitial cells are identified as the major location of vascular alpha 1B-adrenoceptors and may drive vascular remodelling. Br. J. Pharmacol., 137, 21P.
22. MILLIGAN, G. (1999). Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein. Br. J. Pharmacol., 128, 501-510.
23. 1: differential results indicative of a modified membrane receptor. Life Sci., 67, 1285-1296.
24. TRAN, D., DURROUX, T., STELLY, N., SEYER, R., TORDJMANN, T., COMBETTES, L. & CLARET, M. (1999). Visualization of cell surface vasopressin V1a receptors in rat hepatocytes with a fluorescent linear antagonist. J. Histochem. Cytochem., 47, 401-409.
25. VANDENBULCKE, F., NOUEL, D., VINCENT, J.P., MAZELLA, J. & BEAUDET, A. (2000). Ligand-induced internalization of neurotensin in transfected COS-7 cells: differential intracellular trafficking of ligand and receptor. J. Cell Sci., 113, 2963-2975.
26. VON BOHLEN und HALBACH, O. & ALBRECHT, H.D. (2000). Identification of angiotensin IV binding sites in the mouse brain by a fluorescent binding study. Neuroendocrinology, 72, 218-223.