Molecular cloning, sequencing and functional expression of porcine thyrotropin (TSH) receptor cDNA

Clinical Chemistry and Laboratory Medicine

Volumn 41, Issue 6, 2003, Pages 796-803

  Igarashi, Makoto ^a   Nagata, Atsuo ^a  

^a YAMASA CORPORATION   (Japan)

Author keywords

cDNA cloning; Functional expression; Thyroid stimulating antibody; Thyrotropin receptor; TSAb; TSHR

Indexed keywords

COMPLEMENTARY DNA; CYCLIC AMP; MEMBRANE PROTEIN; PRIMER DNA; SIGNAL PEPTIDE; THYROTROPIN RECEPTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHO CELL; CONTROLLED STUDY; GENE EXPRESSION; GENE SEQUENCE; GENETIC CODE; GENETIC TRANSFECTION; GRAVES DISEASE; HUMAN; HUMAN VERSUS ANIMAL COMPARISON; HYDROPHOBICITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; RECEPTOR BINDING; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BINDING, COMPETITIVE; CATTLE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CYCLIC AMP; DNA, COMPLEMENTARY; GENE EXPRESSION; HUMANS; MOLECULAR SEQUENCE DATA; RECEPTORS, THYROTROPIN; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SWINE; THYROTROPIN;

ANIMALIA; BOVINAE; CRICETINAE; CRICETULUS GRISEUS; SUIDAE;

EID: 0037897610     PISSN: 14346621     EISSN: None     Source Type: Journal    
DOI: 10.1515/CCLM.2003.121     Document Type: Article

References (32)

1. Roitt IM, Doniach D, Campbell PN. Autoantibodies in Hashimoto's disease (lymphadenoid goitre). Lancet 1956; 2:820.
2. Smith BR, McLachlan SM, Furmaniak J. Autoantibodies to the thyrotropin receptor. Endocr Rev 1988; 9:106-21.
3. Rickards C, Buckland P, Smith BR, Hall R. The interaction of Graves' IgG with the thyrotropin receptor. FEBS Lett 1981; 127:17-21.
4. Kasagi K, Konishi J, Iida Y, Ikekubo K, Mori T, Kuma K, et al. A new in vitro assay for human thyroid stimulator using cultured thyroid cells: effect of sodium chloride on adenosine 3′, 5′-monophosphate increase. J Clin Endocrinol Metab 1982; 54:108-14.
5. Rapoport B, Greenspan FS, Filetti S, Pepitone M. Clinical experience with a human thyroid cell bioassay for thyroidstimulating immunoglobulin. J Clin Endocrinol Metab 1984; 58:332-8.
6. Kasagi K, Konishi J, Iida Y, Tokuda Y, Arai K, Endo K, et al. A sensitive and practical assay for thyroid-stimulating antibodies using FRTL5 thyroid cells. Acta Endocrinol (Copenh) 1987; 115:30-6.
7. Vitti P, Elisei R, Tonacchera M, Chiovato L, Mancusi F, Rago T, et al. Detection of thyroid-stimulating antibody using Chinese hamster ovary cells transfected with cloned human thyrotropin receptor. J Clin Endocrinol Metab 1993; 76:499-503.
8. Filetti S, Foti D, Costante G, Rapoport B. Recombinant human thyrotropin (TSH) receptor in a radioreceptor assay for the measurement of TSH receptor autoantibodies. J Clin Endocrinol Metab 1991; 72:1096-101.
9. Kakinuma A, Morimoto I, Kuroda T, Eto S, McLachlan SM, Rapoport B. Comparison of recombinant human thyrotropin receptors versus porcine thyrotropin receptors in the thyrotropin binding inhibition assay for thyrotropin receptor autoantibodies. Thyroid 1999; 9:849-55.
10. Costagliola S, Morgenthaler NG, Hoermann R, Badenhoop K, Struck J, Freitag D, et al. Second generation assay for thyrotropin receptor antibodies has superior diagnostics sensitivity for Graves' disease. J Clin Endocrinol Metab 1999; 84:90-7.
11. Murakami M, Mori M. Identification of immunogenic regions in human thyrotropin receptor for immunoglobulin G of patients with Graves' disease. Biochem Biophys Res Comm 1990; 171:512-8.
12. Nagayama Y, Wadsworth HL, Russo D, Chazenbalk GD, Rapoport B. Binding domains of stimulatory and inhibitory thyrotropin (TSH) receptor autoantibodies determined with chimeric TSH-lutropin/chorionic gonadotropin receptors. J Clin Invest 1991; 88:336-40.
13. Sakata S, Ogawa T, Matsui I, Manshouri T, Atassi MZ. Biological activities of rabbit antibodies against synthetic human thyrotropin receptor peptides representing thyotropin binding regions. Biochem Biophys Res Comm 1992; 182:1369-75.
14. Nagy EV, Burch HB, Mahoney K, Lukes YG, Moris III JC, Burman KD. Graves' IgG recognizes linear epitopes in the human thyrotropin receptor. Biochem Biophys Res Comm 1992; 188:28-33.
15. Silversides DW, Houde A, Ethier JF, Lussier JG. Bovine thyrotropin receptor cDNA is characterized by full-length and truncated transcripts. J Mol Endocrinol 1997; 18:101-12.
16. Ochi Y, Kajita Y, Takasu N, Negate A. Sensitive thyroid stimulating antibody (TSAb) assay using polyethylene glycol (PEG) - a review. J Immun Immunochem 2002; 23:461-70.
17. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156-9.
18. Kakinuma A, Chazenbalk G, Filetti S, Mclachlan SM, Rapoport B. Both the 5′ and 3′ noncoding regions of the thyrotropin receptor messenger ribonucleic acid influence the level of receptor protein expression in transfected mammalian cells. Endocrinology 1996; 137:2664-9.
19. Parmentier M, Libert F, Maenhaut C, Lefort A, Gerard C, Perret J, et al. Molecular cloning of the thyrotropin receptor. Science 1989; 246:1620-2.
20. Nagayama Y, Kaufman KD, Seto P, Rapoport B. Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor. Biochem Biophys Res Comm 1989; 165:1184-90.
21. Libert F, Lefort A, Gerard C, Parmentier M, Perret J, Ludgate M, et al. Cloning, sequencing and expression of the human thyrotropin (TSH) receptor: evidence for binding of autoantibodies. Biochem Biophys Res Comm 1989; 165:1250-5.
22. Bockmann J, Winter C, Wittkowski W, Kreutz MR, Bockers TM. Cloning and expression of a brain-derived TSH receptor. Biochem Biophys Res Comm 1997; 238:173-8.
23. Akamizu T, Ikuyama S, Saji M, Kosugi S, Kozak C, McBridge OW, et al. Cloning, chromosomal assignment, and regulation of the rat thyrotropin receptor:expression of the gene is regulated by thyrotropin, agents that increase cAMP levels, and thyroid autoantibodies. Proc Natl Acad Sci USA 1990; 87:5677-81.
24. Stein SA, Oates EL, Hall CR, Grumbles RM, Fernandez LM, Taylor NA, at al. Identification of a point mutation in the thyrotropin receptor of the hyt/hyt hypothyroid mouse. Mol Endocrinol 1994; 8:129-38.
25. Nguyen LQ, Karamanoglu AO, Gerber H, Stein BS, Jameson JL, Kopp P. Cloning of the cat TSH receptor and evidence against an autoimmune etiology of feline hyperthyroidism. Endocrinology 2002; 143:395-402.
26. Oba Y, Hirai T, Yoshiura Y, Kawauchi H, Nagayama Y. Cloning, functional characterization, and expression of thyrotropin receptors in the thyroid of amago salmon (Oncorhynchus rhodurus). Biochem Biophys Res Comm 2000; 276:258-63.
27. Kumar RS, Ijiri S, Kight K, Swanson P, Dittman A, Alak D, et al. Cloning and functional expression of a thyrotropin receptor from the gonads of a vertebrate (bony fish): potential thyroid-independent role for thyrotropin in reproduction. Mol Cell Endocrinol 2000; 167:1-9.
28. Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol 1982; 157: 105-32.
29. Russo D, Chazenbalk GD, Nagayama Y, Wadworth HL, Rapoport B. Site-directed mutagenesis of the human thyrotropin receptor: role of asparagine-linked oligosaccharides in the expression of a functional receptor. Mol Endocrinol 1991; 10:127-40.
30. Kung AW, Lau KS, Kohn LD. Epitope mapping of TSH receptor-blocking antibodies in Graves' disease that appear during pregnancy. J Clin Endocrinol Metab 2001; 86: 3647-53.
31. Cundiff JG, Kaithamana S, Seetharamaiah GS, Baker Jr JR, Prabhakar BS. Studies using recombinant fragment of human TSH receptor reveal apparent diversity in the binding specificities of antibodies that block TSH binding to its receptor or stimulate thyroid hormone production. J Clin Endocrinol Metab 2001; 86:4254-60.
32. Chazenbalk GD, Tanaka K, Mclachlan SM, Rapoport B. On the functional importance of thyrotropin receptor intramolecular cleavage. Endocrinology 1999; 140:4516-20.