Purification of Sarcocystis neurona sporocysts from opossum (Didelphis virginiana) using potassium bromide discontinuous density gradient centrifugation

Parasitology Research

Volumn 90, Issue 2, 2003, Pages 104-109

  Elsheikha, Hany M ^a   Murphy, Alice J ^a   Fitzgerald, Scott D ^a   Mansfield, Linda S ^a   Massey, Jeffrey P ^b   Saeed, Mahdi A ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b MICHIGAN DEPARTMENT OF COMMUNITY HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POTASSIUM BROMIDE; PRIMER DNA; PROPIDIUM IODIDE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL MEMBRANE PERMEABILITY; CELL VIABILITY; CELL WALL; CHEMICAL ANALYSIS; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; FECES ANALYSIS; HOST; IMMUNE RESPONSE; IMMUNITY; IMMUNOLOGY; LIFE CYCLE; NONHUMAN; OOCYST; OPOSSUM; PARASITE ISOLATION; PRIORITY JOURNAL; PURIFICATION; RANDOM AMPLIFIED POLYMORPHIC DNA; RESTRICTION MAPPING; SARCOCYSTIS NEURONA; SPOROZOITE;

ANIMALIA; DIDELPHIDAE; DIDELPHIS VIRGINIANA; PROTOZOA; SARCOCYSTIS NEURONA;

EID: 0037871963     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-002-0789-y     Document Type: Article

References (23)

1. Bukhari Z, Smith HV (1995) Effects of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces. J Clin Microbiol 33:2592-2595
2. Campbell AT, Robertson LJ, Smith HV (1992) Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Appl Environ Microbiol 58:3488-3493
3. Cheadle MA, Dame JB, Greiner EC (2001a) Sporocyst size of isolates of Sarcocystis shed by the Virginia opossum (Didelphis virginiana). Vet Parasitol 95:305-311
4. Cheadle MA, Tanhauser SM, Scase TJ, Dame JB, MacKay RJ, Ginn PE, Greiner EC (2001b) Viability of Sarcocystis neurona sporocysts and dose titration in gamma-interferon knockout mice. Vet Parasitol 95:223-231
5. Cheadle MA, Tanhauser SM, Dame JB, Sellon DC, Hines M, Ginn PE, MacKay RJ, Greiner EC (2001c) The nine-banded armadillo (Dasypus novemcinctus) is an intermediate host for Sarcocystis neurona. Int J Parasitol 31:330-335
6. Cheadle MA, Yowell CA, Sellon DC, Hines M, Ginn PE, Marsh AE, Dame JB, Greiner EC (2001d) The striped skunk (Mephitis mephitis) is an intermediate host for Sarcocystis neurona. Int J Parasitol 31:843-849
7. Dubey JP, Lindsay DS (1998) Isolation in immunodefecient mice of Sarcocystis neurona from opossum (Didelphis virginana) faeces, and its differentiation from Sarcocystis falcatula. Int J Parasitol 28:1823-1828
8. Dubey JP, Speer CA, Fayer R (1989) General biology: In: Sarcocystosis of animal and man. CRC Press, Boca Raton, pp 2-13
9. Dubey JP, Saville WJA, Lindsay DS, Stich RW, Stanek JF, Speer CA, Rosenthal BM, Njoku CJ, Kwok OCH, Shen SK, Reed SM (2000) Completion of the life cycle of Sarcocystis neurona. J Parasitol 86:1276-1280
10. Dubey JP, Mattson DE, Speer CA, Hamir AN, Lindsay DS, Rosenthal BM, Kwok OCH, Baker RJ, Mulrooney DM, Tornquist SJ, Gerros TC (2001) Characteristics of a recent isolate of Sarcocystis neurona (SN7) from a horse and loss of pathogenicity of isolates SN6 and SN7 by passages in cell culture. Vet Parasitol 95:155-166
11. Ellison SP, Greiner E, Dame JB (2001) In vitro culture and synchronous release of Sarcocystis neurona merozoites from host cells. Vet Parasitol 95:251-61
12. Entrala E, Molina-Molina J, Rosales-Lombardo M, Sanchez-Moreno M, Mascaro-Lazcano C (2000) Cryptosporidium parvum: oocysts purification using potassium bromide discontinuous gradient. Vet Parasitol 92:223-226
13. Fenger CK, Granstrom DE, Langemeier JL, Stamper S, Donahue JM, Patterson JS, Gajadher AA, Marteniuk JV, Xiaomin Z, Dubey JP (1995) Identification of opossums (Didelphis virginiana) as the putative definitive host of Sarcocystis neurona. J Parasitol 81:916-919
14. Fenger CK, Granstrom DE, Gajadher AA, Williams NM, McCrillis SA, Stamper S, Langemeier JL, Dubey JP (1997) Experimental induction of equine protozoal myeloencephalitis in horses using Sarcocystis sp. sporocysts from the opossum (Didelphis virginiana). Vet Parasitol 68:199-213
15. Gardiner CH, Fayer R, Dubey JP (1988) An atlas of protozoan parasites in animal tissues. Agriculture Handbook, vol 651. USDA, Agricultural Research Service, pp 40-45
16. Griffiths JK, Moore R, Dooley S, Keusch GT, Tzipori S (1994) Cryptosporidium parvum infection of Caco-2 cell monolayers induces an apical monolayer defect, selectively increases transmonolayer permeability, and causes epithelial cell death. Infect Immun 62:4506-4514
17. Hamir AN, Dubey JP (2001) Myocarditis and encephalitis associated with Sarcocystis neurona infection in raccoons (Procyon lotor). Vet Parasitol 95:335-340
18. Lindsay DS, McKown R, Upton SJ, McAllister CT, Toivio-Kinnucan MA, Veatch JK, Blagburn BL (1996) Prevalence and identity of Sarcocystis infections in armadillos (Dasypus novemcinctus). J Parasitol 82:518-520
19. Mansfield LS, Schott HC, Murphy AJ, Rossano MG, Tanhauser SM, Patterson JS, Nelson K, Ewart SL, Marteniuk JV, Bowman D, Kaneene JB (2001) Comparison of Sarcocystis neurona isolates derived from horse neural tissue. Vet Parasitol 95:167-178
20. Marsh AE, Johnson PJ, Ramos-Vara J, Johnson GC (2001) Characterization of a Sarcocystis neurona isolate from a Missouri horse with equine protozoal myeloencephalitis. Vet Parasitol 95:143-154
21. Murphy AJ, Mansfield LS (1999) Simplified technique for isolation, excystation, and culture of Sarcocystis species from opossums. J Parasitol 85:979-981
22. Taghi-Kilani R, Sekla L (1987) Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients. Am J Trop Med Hyg 36:505-508
23. Tanhauser SM, Yowell CA, Cutler TJ, Greiner EC, MacKay RJ, Dame JB (1999) Multiple DNA markers differentiate Sarcocystis neurona and Sarcocystis falcatula. J Parasitol 85:221-228