The contribution of the SPCA1 Ca2+ pump to the Ca2+ accumulation in the Golgi apparatus of HeLa cells assessed via RNA-mediated interference

Biochemical and Biophysical Research Communications

Volumn 306, Issue 2, 2003, Pages 430-436

  Van Baelen, Kurt ^a   Vanoevelen, Jo ^a   Callewaert, Geert ^a   Parys, Jan B ^a   De Smedt, Humbert ^a   Raeymaekers, Luc ^a   Rizzuto, Rosario ^b   Missiaen, Ludwig ^a   Wuytack, Frank ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b UNIVERSITY OF FERRARA   (Italy)

Author keywords

Calcium oscillations; Calcium transport ATPase; Endoplasmic reticulum; Golgi apparatus; Intracellular calcium; SERCA; SPCA1

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATASE (CALCIUM); AEQUORIN; ANTIBODY; ANTIBODY SPCA1; CALCIUM; PHOTOPROTEIN; RNA; SECRETORY PATHWAY CALCIUM ADENOSINE TRIPHOSPHATASE; UNCLASSIFIED DRUG; ATP2C1 PROTEIN, HUMAN; ENZYME INHIBITOR; RECOMBINANT PROTEIN; THAPSIGARGIN;

ARTICLE; CALCIUM SIGNALING; CALCIUM TRANSPORT; CELL ORGANELLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME DEFICIENCY; ENZYME SUBUNIT; FRACTIONATION; FUNCTIONAL ASSESSMENT; GOLGI COMPLEX; HELA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MEASUREMENT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL PROCESSING; CELL MEMBRANE; CHEMISTRY; ENDOPLASMIC RETICULUM; FLUORESCENCE MICROSCOPY; GENE VECTOR; GENETIC TRANSFECTION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; KINETICS; METABOLISM; PHYSIOLOGY; RNA INTERFERENCE;

CALCIUM; CALCIUM-TRANSPORTING ATPASES; CELL MEMBRANE; ENDOPLASMIC RETICULUM; ENZYME INHIBITORS; GENETIC VECTORS; GOLGI APPARATUS; HELA CELLS; HUMANS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; KINETICS; MICROSCOPY, FLUORESCENCE; RECOMBINANT PROTEINS; RNA INTERFERENCE; THAPSIGARGIN; TRANSFECTION;

EID: 0037870343     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)00977-X     Document Type: Article

References (26)

1. Berridge M.J. Inositol trisphosphate and calcium signalling. Nature. 361:1993;315-325.
2. Pozzan T., Rizzuto R., Volpe P., Meldolesi J. Molecular and cellular physiology of intracellular calcium stores. Physiol. Rev. 74:1994;595-636.
3. Lytton J., Westlin M., Hanley M.R. Thapsigargin inhibits the sarcoplasmic or endoplasmic reticulum Ca-ATPase family of calcium pumps. J. Biol. Chem. 266:1991;17067-17071.
4. Yoshimoto A., Nakanishi K., Anzai T., Komine S. Effects of inositol 1,4,5-trisphosphate on calcium release from the endoplasmic reticulum and Golgi apparatus in mouse mammary epithelial cells: a comparison during pregnancy and lactation. Cell. Biochem. Funct. 8:1990;191-198.
5. 2+ regulation in epithelial LLC-PK1 cells. Am. J. Physiol. 268:1995;C1133-C1140.
6. 2+ store, with functional properties distinct from those of the endoplasmic reticulum. EMBO J. 17:1998;5298-5308.
7. Surroca A., Wolff D. Inositol 1,4,5-trisphosphate but not ryanodine-receptor agonists induces calcium release from rat liver Golgi apparatus membrane vesicles. J. Membr. Biol. 177:2000;243-249.
8. 2+ storage in the Golgi. J. Cell Biol. 145:1999;279-289.
9. Rojas P., Surroca A., Orellana A., Wolff D. Kinetic characterization of calcium uptake by the rat liver Golgi apparatus. Cell Biol. Int. 24:2000;229-233.
10. Taylor R.S., Jones S.M., Dahl R.H., Nordeen M.H., Howell K.E. Characterization of the Golgi complex cleared of proteins in transit and examination of calcium uptake activities. Mol. Biol. Cell. 8:1998;1911-1931.
11. Gunteski-Hamblin A.M., Clarke D.M., Shull G.E. Molecular cloning and tissue distribution of alternatively spliced mRNAs encoding possible mammalian homologues of the yeast secretory pathway calcium pump. Biochemistry. 31:1992;7600-7608.
12. Van Baelen K., Vanoevelen J., Missiaen L., Raeymaekers L., Wuytack F. The Golgi PMR1 P-type ATPase of Caenorhabditis elegans. Identification of the gene and demonstration of calcium and manganese transport. J. Biol. Chem. 276:2001;10683-10691.
13. 2+-ATPase defective in Hailey-Hailey disease. J. Biol. Chem. 277:2002;6422-6427.
14. 2+ ATPase family. Cell. 58:1989;133-145.
15. 2+-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol. Biol. Cell. 3:1992;633-654.
16. 2+-ATPase in yeast Golgi, has properties distinct from sarco/endoplasmic reticulum and plasma membrane calcium pumps. J. Biol. Chem. 272:1997;9895-9901.
17. 2+ store. J. Biol. Chem. 276:2001;39161-39170.
18. 2+ signals in Pmr1-GFP-expressing COS-1 cells with functional endoplasmic reticulum. Biochem. Biophys. Res. Commun. 294:2002;249-253.
19. 2+ store in A7r5 and 16HBE14o- cells. J. Biol. Chem. 277:2002;6898-6902.
20. Sharp P.A. RNA interference-2001. Genes Dev. 15:2001;485-490.
21. Brummelkamp T.R., Bernards R., Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science. 296:2002;550-553.
22. Parys J.B., De Smedt H., Missiaen L., Bootman M.D., Sienaert I., Casteels R. Rat basophilic leukemia cells as model system for inositol 1,4,5-trisphosphate receptor IV, a receptor of the type II family: functional comparison and immunological detection. Cell Calcium. 17:1995;239-249.
23. Rizzuto R., Brini M., Bastianutto C., Marsault R., Pozzan T. Photoprotein-mediated measurement of calcium ion concentration in mitochondria of living cells. Methods Enzymol. 260:1995;417-428.
24. c). A critical evaluation. J. Biol. Chem. 270:1995;9896-9903.
25. Barr F.A. A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins. Curr. Biol. 9:1999;381-384.
26. 2+ mobilization from the intracellular stores of single histamine-stimulated HeLa cells. J. Physiol. 450:1992;163-178.