Improved protocol for SAGE tag-to-gene allocation

BioTechniques

Volumn 34, Issue 6, 2003, Pages 1212-1219

  Kannbley, Ute ^a   Kapinya, Krisztian ^a   Dirnagl, Ulrich ^a   Trendelenburg, George ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DATABASE SYSTEMS; DNA SEQUENCES; GENETIC ENGINEERING; NETWORK PROTOCOLS; PATTERN RECOGNITION SYSTEMS;

GENE TRANSCRIPTION;

BIOMEDICAL ENGINEERING;

COMPLEMENTARY DNA; DNA FRAGMENT; STREPTAVIDIN;

3' UNTRANSLATED REGION; ARTICLE; DNA ISOLATION; GENE EXPRESSION; GENE SEQUENCE; INFORMATION PROCESSING; POLYMERASE CHAIN REACTION; TECHNIQUE;

SALVIA;

EID: 0037839757     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03346st04     Document Type: Article

References (11)

1. Velculescu, V.E., L. Zhang, B. Vogelstein, and K.W. Kinzler. 1995. Serial analysis of gene expression. Science 270:484-487.
2. Velculescu, V.E., B. Vogelstein, and K.W. Kinzler. 2000. Analysing uncharted transcriptomes with SAGE. Trends Genet. 16:423-425.
3. Trendelenburg, G., K. Prass, J. Priller, K. Kapinya, A. Polley, C. Muselmann, K. Ruscher, U. Kannbley, et al. 2002. Serial analysis of gene expression identifies metallothionein II as major neuroprotective gene in mouse focal cerebral ischemia. J. Neurosci. 22:5879-5888.
4. Van den Berg, A., J. van der Leij, and S. Poppema. 1999. Serial analysis of gene expression: rapid RT-PCR analysis of unknown tags. Nucleic Acids Res. 27:e17.
5. Chen, J.J., J.D. Rowley, and S.M. Wang. 1999. Generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification. Proc. Natl. Acad. Sci. USA 97:349-353.
6. Frohman, M.A. 1994. On beyond classic RACE (rapid amplification of cDNA ends). PCR Methods Appl. 4:S40-S58.
7. Chen, J., S. Lee, G. Zhou, and S.M. Wang. 2002. High-throughput GLGI procedure for converting a large number of serial analysis of gene expression tag sequences into 3′ complementary cDNAs. Genes Chromosomes Cancer 33:252-261.
8. Lal, A., A.E. Lash, S.F. Altschul, V.E. Velculescu, L. Zhang, R.E. McLendon, M.A. Marra, C. Prange, et al. 1999. A public database for gene expression in human cancers. Cancer Res. 59:5403-5407.
9. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
10. Fieschi, J., P. Niccoli, C. Camilla, P. Chames, M. Chartier, and D. Baty. 1996. Polymerase chain reaction-based site-directed mutagenesis using magnetic beads. Anal. Biochem. 234:210-214.
11. Chen, J., R. Johnson, and M. Griffiths. 1998. Detection of verotoxigenic Escherichia coli by magnetic capture-hybridisation PCR. Appl. Environ. Microbiol. 64:147-152.