Cell-free DNA is released from tumor cells upon cell death: A study of tissue cultures of tumor cell lines

Journal of Clinical Laboratory Analysis

Volumn 17, Issue 4, 2003, Pages 103-107

  Li, Chia Ni ^a   Hsu, Hui Lun ^a   Wu, Tsu Lan ^a   Tsao, Kuo Chien ^a   Sun, Chien Feng ^a   Wu, James T ^b,c  

^a CHANG GUNG MEMORIAL HOSPITAL   (Taiwan)

^b UNIVERSITY OF UTAH HEALTH SCIENCES CENTER   (United States)

^c ARUP LABORATORIES   (United States)

Author keywords

Cell death; Cell free DNA; Necrosis; Tissue culture; Tumor cell line

Indexed keywords

CA 125 ANTIGEN; DNA;

ARTICLE; CANCER TISSUE; CELL COUNT; CELL DEATH; CELL FREE SYSTEM; CELL GROWTH; CELL VIABILITY; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOPATHOLOGY; DNA DETERMINATION; HISTOPATHOLOGY; HUMAN; HUMAN CELL; LABORATORY TEST; TISSUE CULTURE; TUMOR CELL LINE;

CA-125 ANTIGEN; CELL LINE, TUMOR; CELL-FREE SYSTEM; DNA, NEOPLASM; FEMALE; HUMANS; MALE; NECROSIS; NEOPLASMS; RECEPTOR, ERBB-2; TUMOR MARKERS, BIOLOGICAL;

EID: 0037829605     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcla.10081     Document Type: Article

References (11)

1. Jahr S, Hentze H, Englisch S, et al. DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res 2001;61:1659-1665.
2. Stroun M, Anker P, Lyautey J, Lederrey C, Maurice PA. Isolation and characterization of DNA from the plasma of cancer patients. Eur J Cancer Clin Oncol 1987;23:707-712.
3. Fournie GJ, Courtin JP, Laval F, et al. Plasma DNA as a marker of cancerous cell death. Investigations in patients suffering from lung cancer and in nude mice bearing human tumours. Cancer Lett 1995;91:221-227.
4. Anker P, Mulcahy H, Chen XQ, Stroun M. Detection of circulating tumour DNA in the blood (plasma/serum) of cancer patients. Cancer Metastasis Rev 1999;18:65-73.
5. Shapiro B, Chakrabarty M, Cohn EM, Leon SA. Determination of circulating DNA levels in patients with benign or malignant gastrointestinal disease. Cancer 1983;51:2116-2120.
6. Wu T-L, Chia J-H, Zhang D, Tsao K-C, Sun C-F, Wu JT. Cell-free DNA: measurement in various carcinomas and establishment of normal reference range. Clin Chim Acta 2002;321:77-87.
7. Fournie GJ, Martres F, Pourrat JP, Alary C, Rumeau M. Plasma DNA as cell death marker in elder patients. Gerontology 1993;39:215-221.
8. Fournie GJ, Courtin JP, Chale LF, et al. Plasma DNA as a marker of cancerous cell death. Investigations in patients suffering from lung cancer and in nude mice bearing human tumours. Cancer Lett 1995;91:221-227.
9. Wu JT. c-erbB2 oncoprotein and its soluble ectodomain: a new potential tumor marker for prognosis, early detection and monitoring patients undergoing Herceptin treatment. Clin Chim Acta 2002;322:11-19.
10. Sjostrom J, Alfthan H, Joensuu H, Stenman UH, Lundin J, Blomqvist C. Serum tumour markers CA 15-3, TPA, TPS, hCGbeta and TATI in the monitoring of chemotherapy response in metastatic breast cancer. Scand J Clin Lab Invest 2001;61:431-441.
11. Sun C-F, Haven TR, Wu T-L, Tsao K-C, Wu JT. Serum total homocysteine increases with the rapid proliferation rate of tumor cells and decline upon cell death: a potential new tumor marker. Clin Chim Acta 2002;321:55-62.