Monitoring the uptake of protein-derived peptides by Porphyromonas gingivalis with fluorophore-labeled substrates

Current Microbiology

Volumn 47, Issue 1, 2003, Pages 1-4

  Yoshioka, Masami ^a   Grenier, Daniel ^a   Mayrand, Denis ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

4 (2 AMINOETHYL)BENZENESULFONYL FLUORIDE; ALBUMIN; BENZENE DERIVATIVE; BOVINE SERUM ALBUMIN; CATHEPSIN B; CATHEPSIN B INHIBITOR II; FLUORESCENT DYE; PEPTIDE; PHOSPHATE; PROTEIN DERIVED PEPTIDE; PROTEINASE INHIBITOR; UNCLASSIFIED DRUG;

ANAEROBIC GROWTH; ARTICLE; BACTERIAL STRAIN; CHEMICAL LABELING; FLUORESCENCE; NONHUMAN; PH; PORPHYROMONAS GINGIVALIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN TRANSPORT; CULTURE MEDIUM; GROWTH, DEVELOPMENT AND AGING; METABOLISM; OSMOLARITY;

BACTERIA (MICROORGANISMS); BOVINAE; NEGIBACTERIA; PORPHYROMONAS; PORPHYROMONAS GINGIVALIS;

CULTURE MEDIA; FLUORESCENCE; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; OSMOLAR CONCENTRATION; PEPTIDES; PORPHYROMONAS GINGIVALIS; PROTEASE INHIBITORS; SERUM ALBUMIN, BOVINE;

EID: 0037824581     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-002-3916-1     Document Type: Article

References (12)

1. Banbula A, Bugno M, Goldstein J, Yen J, Nelson D, Travis J, Potempa J (2000) Emerging family of proline-specific peptidases of Porphyromonas gingivalis: purification and characterization of serine dipeptidyl peptidase, a structural and functional homologue of mammalian prolyl dipeptidyl peptidase IV. Infect Immun 68:1176-1182
2. Brochu V, Grenier D, Nakayama K, Mayrand D (2001) Acquisition of iron from human transferrin by Porphyromonas gingivalis: a role for Arg- and Lys-gingipain activities. Oral Microbiol Immunol 16:79-87
3. Chen YY, Cross KJ, Paolini, RA, Fielding JE, Slakeski N, Reynolds EC (2002) CPG70 is a novel basic metallocarboxypeptidase with C-terminal polycystic kidney disease domains from Porphyromonas gingivalis. J Biol Chem 277:23433-23440
4. Dashper SG, Kandasamy S, O'Brien-Simpson N, Reynolds EC (1998) Amino acid and peptide uptake by Porphyromonas gingivalis. Abstracts of the Annual Meeting of the International Association for Dental Research. J Dent Res 77:1133
5. Gharbia SE, Shah HN (1991) Utilization of aspartate, glutamate, and their corresponding peptides by Fusobacterium nucleatum subspecies and Porphyromonas gingivalis. Curr Microbiol 22:159-163
6. Grenier D, Imbeault S, Plamondon P, Grenier G, Nakayama K, Mayrand D (2001) Role of gingipains in growth of Porphyromonas gingivalis in the presence of human serum albumin. Infect Immun 69:5166-5172
7. Holt S, Kesavalu L, Walker S, Genco CA (1999) Virulence factors of Porphyromonas gingivalis. Periodontology 2000 20:168-238
8. Masuda K, Yoshioka M, Hinode D, Nakamura R (2002) Purification and characterization of arginine carboxypeptidase produced by Porphyromonas gingivalis. Infect Immun 70:1807-1815
9. Miller P, Batten J, Curtis MA (1994) Development of a simple chemically defined medium for Porphyromonas gingivalis: requirement for α-ketoglutarate. FEMS Microbiol Lett 140:125-130
10. Payne JW, Nisbet TM (1980) Limitations to the use of radioactively labelled substrates for studying peptide transport in microorganisms. FEBS Lett 119:73-76
11. Shi Y, Ratnayake DB, Okamoto K, Abe N, Yamamoto K et al. (1999) Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. J Biol Chem 274:17955-17960
12. Takahashi N, Sato T (2001) Preferential utilization of dipeptides by Porphyromonas gingivalis. J Dent Res 80:1425-1429