A B cell-based sensor for rapid identification of pathogens

Science

Volumn 301, Issue 5630, 2003, Pages 213-215

  Rider, Todd H ^a   Petrovick, Martha S ^a   Nargi, Frances E ^a   Harper, James D ^a   Schwoebel, Eric D ^a   Mathews, Richard H ^a   Blanchard, David J ^a   Bortolin, Laura T ^a   Young, Albert M ^a   Chen, Jianzhu ^a   Hollis, Mark A ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; SENSORS; VIRUSES;

PATHOGENS;

PATHOLOGY;

GENETICS; PATHOGEN;

ARTICLE; B LYMPHOCYTE; COLONY FORMING UNIT; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; FOOD QUALITY; GENETIC ENGINEERING; MICROORGANISM DETECTION; NONHUMAN; PRIORITY JOURNAL; SENSOR; WATER QUALITY; BACILLUS ANTHRACIS; BACTERIAL COUNT; BACTERIUM; BIOSYNTHESIS; CELL LINE; ESCHERICHIA COLI O157; FOOT AND MOUTH DISEASE VIRUS; GENETIC PROCEDURES; GENETIC TRANSFECTION; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; LIGHT; MICROBIOLOGICAL EXAMINATION; SENSITIVITY AND SPECIFICITY; TIME; VENEZUELAN EQUINE ENCEPHALOMYELITIS ALPHAVIRUS; VIRUS; YERSINIA PESTIS;

AEQUORIN; BACTERIUM ANTIBODY; LYMPHOCYTE ANTIGEN RECEPTOR; VIRUS ANTIBODY;

AEQUORIN; ANTIBODIES, BACTERIAL; ANTIBODIES, VIRAL; B-LYMPHOCYTES; BACILLUS ANTHRACIS; BACTERIA; BACTERIOLOGICAL TECHNIQUES; BIOSENSING TECHNIQUES; CELL LINE; COLONY COUNT, MICROBIAL; ENCEPHALITIS VIRUS, VENEZUELAN EQUINE; ESCHERICHIA COLI O157; FOOT-AND-MOUTH DISEASE VIRUS; IMMUNOGLOBULIN VARIABLE REGION; LIGHT; RECEPTORS, ANTIGEN, B-CELL; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TRANSFECTION; VIRUSES; YERSINIA PESTIS;

EID: 0037815127     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1084920     Document Type: Article

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17. We appreciate the following generous donations: M12g3R B cell line, P. Tucker (University of Texas, Austin); pCMV.AEQ.IRES.NEO plasmid, D. Button (Stanford University); VKExpress, Invitrogen Corporation; B. anthracis Sterne, antibody to orthopoxvirus, and TC-83 VEE, G. Ludwig (U.S. Army Medical Research Institute of Infectious Diseases); antibody to Y. pestis sequence and inactivated bacteria, Naval Medical Research Center; antibody to B. anthracis spore, J. Kearney (University of Alabama, Birmingham); and antibody to VEE and inactivated VEE, J. Roehrig (Centers for Disease Control and Prevention, Fort Collins, CO). We are grateful for the advice and assistance of M. Boes, B. Cho, L. Filip, S. Lakdawala, A. Lee, K. Molvar, S. T. Palmacci, G. Paradis, L. Parameswaran, T. Schmidt, C. Wang, and C. Whitehurst. We greatly appreciate the assistance and support of E. Henchal, A. Mateczun, L. Peruski. and J. Gebhardt. This work was sponsored by A. Rudolph of the Defense Advanced Research Projects Agency's Defense Sciences Office and by A. Senecal of the U.S. Army Soldier and Biological Chemical Command's Combat Feeding Program under Air Force contract number F19628-00-C-0002. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the Department of Defense.