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Volumn 286, Issue 5442, 1999, Pages 1129-1132

Polycationic peptides from diatom biosilica that direct silica nanosphere formation

Author keywords

[No Author keywords available]

Indexed keywords

LYSINE DERIVATIVE; NANOPARTICLE; PEPTIDE HORMONE; POLYCATION; SILAFFIN; SILICIC ACID; SILICON DIOXIDE; UNCLASSIFIED DRUG;

EID: 0037814710     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5442.1129     Document Type: Article
Times cited : (1277)

References (29)
  • 1
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    • S. Mann, Nature 365, 499 (1993).
    • (1993) Nature , vol.365 , pp. 499
    • Mann, S.1
  • 14
    • 0344038632 scopus 로고    scopus 로고
    • note
    • 2O to remove free silicic acid and phosphate. Washed pellets were resuspended in 10 μl of 1 M NaOH and silica was dissolved by incubation at 95°C for 30 min. Silicium concentrations were determined in these solutions by the β-silicomolybdate method (19).
  • 15
    • 0344469779 scopus 로고    scopus 로고
    • note
    • 2O to a final protein concentration of 10 to 30 μmol/ml.
  • 27
    • 0022186670 scopus 로고
    • Protein concentration was determined by the bicinchoninic acid assay as described [P. K. Smith et al., Anal. Biochem. 150, 76 (1985)].
    • (1985) Anal. Biochem. , vol.150 , pp. 76
    • Smith, P.K.1
  • 28
    • 0344469776 scopus 로고    scopus 로고
    • note
    • Peptide concentration was determined after acid hydrolysis by HPLC analysis of the PTC amino acid derivatives.
  • 29
    • 0345332507 scopus 로고    scopus 로고
    • note
    • We thank G. Lehmann and E. Hochmuth for technical assistance; T. Maurer and H. R. Kalbitzer for NMR analysis; and F. Siedler and H. Sarioglou for MS/MS analysis. We are indebted to G. Wanner for fieldemission SEM analysis and R. Wetherbee for help with TEM analysis. We greatfully acknowledge W. Tanner for critically reading the manuscript. This work was supported by the Deutsche Forschungsge-meinschaft (SFB 521).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.