Four-dimensional multiphoton microscopy with time-correlated single-photon counting

Applied Optics

Volumn 39, Issue 34, 2000, Pages 6306-6311

  Schönle, Andreas ^a   Glatz, Markus ^a   Hell, Stefan W ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AVALANCHE DIODES; CELLS; ENERGY TRANSFER; FLUORESCENCE; OPTICAL MICROSCOPY; PHOTODIODES; PHOTONS; PROTEINS; PULSED LASER DEPOSITION; SIGNAL TO NOISE RATIO; STATISTICAL METHODS;

SINGLE-PHOTON COUNTING;

IMAGING SYSTEMS;

EID: 0037812528     PISSN: 1559128X     EISSN: 21553165     Source Type: Journal    
DOI: 10.1364/AO.39.006306     Document Type: Article

References (17)

1. A. Draaijer, R. Sanders, and H. C. Gerritsen, “Fluorescence lifetime imaging, a new tool in confocal microscopy,” in Handbook of Biological Confocal Microscopy, J. Pawley, ed. (Plenum, New York, 1995), pp. 491-505.
2. J. R. Lakowicz, H. Szmaczinski, and K. Nowaczyk, “Fluorescence lifetime imaging,” Proc. Natl. Acad. Sci. USA 89, 1271-1275 (1992).
3. J. R. Lakowicz and K. W. Berndt, “Lifetime-selective fluorescence imaging using a rf phase-sensitive camera,” Rev. Sci. Instrum. 62(7), 1727-1734 (1991).
4. G. Marriott, R. M. Clegg, D. J. Arndt-Jovin, and T. M. Jovin, “Time-resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging,” Biophys. J. 60, 1374-1387 (1991).
5. C. G. Morgan, A. C. Mitchell, and C. G. Murray, “Prospects for confocal imaging based on nanosecond fluorescence decay time,” J. Microsc. 165, 49-60 (1991).
6. A. Squire, P. J. Verveer, and P. I. H. Bastiaens, “Multiple frequency fluorescence imaging microscopy,” J. Microsc. 197, 136-149 (2000).
7. X. F. Wang, T. Uchida, D. M. Coleman, and S. Minami, “A two-dimensional fluorescence lifetime imaging system using a gated image intensifier,” Appl. Spectrosc. 45, 360-366 (1991).
8. X. F. Wang, S. Kitajima, T. Uchida, D. M. Coleman, and S. Minami, “Time-resolved fluorescence microscopy using multichannel photon counting,” Appl. Spectrosc. 44, 25-30 (1990).
9. M. Straub and S. W. Hell, “Fluorescence lifetime threedimensional microscopy with picosecond precision using a multifocal multiphoton microscope,” Appl. Phys. Lett. 73, 1769-1771 (1998).
10. M. Dyba, T. A. Klar, S. Jakobs, and S. W. Hell, “Ultrafast dynamics microscopy,” Appl. Phys. Lett. 77, 597-599 (2000).
11. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73-76 (1990).
12. J. Sytsma, J. M. Vroom, and H. C. Gerritsen, “Time-gated fluorescence lifetime imaging and microvolume spectroscopy using two-photon excitation,” J. Microsc. 191, 39-42 (1998).
13. W. H. Press, B. P. Flannery, S. A. Teukolsky, and W. T. Vet-terling, Numerical Recipes in C, 2nd. ed. (Cambridge U. Press, Cambridge, 1993).
14. M. Schrader, U. G. Hofmann, and S. W. Hell, “Ultrathin fluorescent layers for monitoring the axial resolution in confocal and two-photon fluorescence microscopy,” J. Microsc. 191, 135-140 (1998).
15. W. Becker, H. Hickl, C. Zander, K. H. Drexhage, M. Sauer, S. Siebert, and J. Wolfrum, “Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (TCSPC),” Rev. Sci. Instrum. 70(3), 1835-1841 (1999).
16. S. W. Hell, A. Utz, P. E. Hanninen, and E. Soini, “Pulsed laser fluorophore deposition: a method for measuring the axial resolution in two-photon fluorescence microscopy,” J. Opt. Soc. Am. A 12, 2072-2076 (1995).
17. P. I. H. Bastiaens, I. V. Majoul, P. J. Verveer, H. D. Soling, and T. M. Jovin, “Imaging the intracellular trafficking and state of the AB5 quaternary structure of cholera toxin,” EMBO 15, 4246-4253 (1996).