Colonic mucin: Methods of measuring mucus thickness

Proceedings of the Nutrition Society

Volumn 62, Issue 1, 2003, Pages 237-243

  Strugala, Vicki ^a,b   Allen, Adrian ^a   Dettmar, Peter W ^b   Pearson, Jeffrey P ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b RECKITT BENCKISER   (United Kingdom)

Author keywords

Dietary fibre; Mucus thickness; Rat colon

Indexed keywords

GLYCOPROTEIN; MUCIN;

CHEMICAL ANALYSIS; CHEMICAL COMPOSITION; COLON SECRETION; CONFERENCE PAPER; DIETARY FIBER; FROZEN SECTION; HISTOLOGY; MUCUS; MUCUS SECRETION; PERIODIC ACID SCHIFF STAIN;

ANIMALS; COLON; DIETARY FIBER; HUMANS; INTESTINAL MUCOSA; MUCINS; PERIODIC ACID-SCHIFF REACTION; RATS; RATS, WISTAR;

ANIMALIA;

EID: 0037799913     PISSN: 00296651     EISSN: None     Source Type: Journal    
DOI: 10.1079/PNS2002205     Document Type: Conference Paper

References (43)

1. Allen A (1981) Structure and function of gastrointestinal mucus. In Physiology of the Gastrointestinal Tract, pp. 617-639 [LR Leonard, editor]. New York: Raven Press.
2. Allen A (1989) Gastrointestinal mucus. In Handbook of Physiology - The Gastrointestinal System, vol. 3, pp. 359-382 [JG Forte, editor]. Bethesda, ML: American Physiological Society.
3. Bentley R & Meganathan R (1982) Biosynthesis of vitamin K (menaquinone) in bacteria. Microbiological Reviews 46, 241-280.
4. Bowman WC & Rand MJ (1980) Textbook of Pharmacology. Oxford: Blackwell Scientific Publications.
5. Brownlee IA, Havler ME, Dettmar PW, Allen A & Pearson JP (2003) Colonic mucus: secretion and turnover in relation to dietary fibre intake. Proceedings of the Nutrition Society 62, 245-249.
6. Burkitt DP, Walker ARP & Painter NS (1972) Effect of dietary fibre on stools and transit times, and its roles in the causation of disease. Lancet ii, 1408-1411.
7. Clausen MR & Mortensen PB (1995) Kinetic studies in colonocyte metabolism of short-chain fatty acids and glucose in ulcerative colitis. Gut 37, 684-689.
8. Cross CE, Halliwell B & Allen A (1984) Antioxidant protection -a function of tracheo-bronchial and gastrointestinal mucus. Lancet i, 1328-1330.
9. Cummings JH (1993) The effect of dietary fiber on faecal weight and composition. In Handbook of Dietary Fiber in Human Nutrition, pp. 263-349 [GA Spiller, editor]. Boca Raton, FL: CRC Press.
10. Cummings JH, Bingham SA, Heaton KW & Eastwood MA (1992) Faecal weight, colon cancer risk, and dietary intake of non-starch polysaccharides (dietary fiber). Gastroenterology 103, 1783-1789.
11. Cummings JH, Hill MJ, Jenkins DJA, Pearson JR & Wiggins HS (1976) Changes in fecal composition and colonic function due to cereal fiber. American Journal of Clinical Nutrition 29, 1468-1473.
12. Davidson MH & McDonald A (1998) Fiber: forms and functions. Nutrition Research 18, 617-624.
13. Englyst HN, Quigley ME & Hudson GJ (1994) Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic, high-performance liquid chromatographic or spectrophotometric measurement of constituent sugars. Analyst 119, 1497-1509.
14. Garland CD, Nash GV & McMeekin TA (1982) The preservation of mucus and surface-associated microorganisms using acrolein vapor fixation. Journal of Microscopy - Oxford 128, 307-312.
15. Goodlad RA, Ratcliffe B, Fordham JP & Wright NA (1989) Does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats? Gut 30, 820-825.
16. Holm L & Flemström G (1990) Microscopy of acid transport at the gastric surface in vivo. Journal of Internal Medicine 228, 91-95.
17. Holm-Rutili L & Obrink KJ (1985) Rat gastric mucosal microcirculation in vivo. American Journal of Physiology 11, G741-G746.
18. Hunter AC (1989) Mucus and mucosal bicarbonate in the gastrointestinal tract. PhD thesis, University of Newcastle upon Tyne.
19. Izhar M, Nuchamowitz Y & Mirelman D (1982) Adherence of Shigella flexneri to guinea pig intestinal cells is mediated by mucosal adhesion. Infection and Immunity 35, 1110-1118.
20. Jordan N, Newton J, Pearson J & Allen A (1998) A novel method for the visualization of the in situ mucus layer in rat and man. Clinical Science 95, 97-106.
21. Kerss S, Allen A & Garner A (1982) A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: influence of feeding, prostaglandin, N-acetylcysteine and other agents. Clinical Science 63, 187-195.
22. McQueen S, Allen A & Garner A (1984) Measurement of gastric and duodenal mucus gel thickness. In Mechanisms of Mucosal Protection in the Upper Gastrointestinal Tract, pp. 215-219 [A Allen, editor]. New York: Raven Press.
23. McQueen S, Hutton D, Allen A & Garner A (1983) Gastric and duodenal surface mucus gel thickness in rat: effects of prostaglandins and damaging agents. American Journal of Physiology 245, G388-G393.
24. Mathers JC, Fernandez F, Hill MJ, McCarthy PT, Shearer MJ & Oxley A (1990) Dietary modification of potential vitamin K supply from enteric bacterial menaquinones in rats. British Journal of Nutrition 63, 639-652.
25. Morris GP (1985) The myth of the mucus barrier. Gastroenterologique Clinique et Biologique 9, 1006-1107.
26. Morris GP, Harding RK & Wallace JL (1984) A functional model for extracellular gastric mucus in the rat. Virchows Archive 46, 239-251.
27. Morris GP & Wallace JL (1981) The roles of ethanol and of acid in the production of gastric mucosal erosions in rats. Virchows Archive 38, 23-38.
28. Neutra MR & Forstner JF (1987) Gastrointestinal mucus: synthesis, secretion, and function. In Physiology of the Gastrointestinal Tract, vol. 2, pp. 975-1009 [LR Johnson, editor]. New York: Raven Press.
29. Newton J, Jordan N, Pearson JP, Allen A & James OJ (1998) Thinning of the adherent gastric antral and duodenal mucus gel layer in H. pylori positive subjects with advancing age. Gut 42, A80.
30. Nyman M & Asp N-G (1982) Fermentation of dietary fibre components in the rat intestinal tract. British Journal of Nutrition 47, 357-366.
31. Nyman M, Asp N-G, Cummings JH & Wiggins H (1986) Fermentation of dietary fibre in the intestinal tract; comparison between man and rat. British Journal of Nutrition 55, 487-496.
32. Pullan RD, Thomas GAO, Rhodes M, Newcombe RG, Rhodes GT, Allen A & Rhodes J (1994) Thickness of the adherent mucus gel on colonic mucosa in humans and its relevance to colitis. Gut 35, 353-359.
33. Rozee KR, Cooper D, Lam K & Costerton JW (1982) Microbialflora of the mouse ileum mucous layer and epithelial surface. Applied and Environmental Microbiology 43, 1451-1463.
34. Sababi M, Nilsson E & Holm L (1995) Mucus and alkali secretion in the rat duodenum - effects of indomethacin, N-omega-nitro-Larginine, and luminal acid. Gastroenterology 109, 1526-1534.
35. Schade C, Flemström G & Holm L (1994) Hydrogen ion concentration in the mucus layer on top of acid-stimulated and acidinhibited rat gastric mucosa. Gastroenterology 107, 180-188.
36. Sellers LA, Allen A & Bennett MK (1987) Formation of a fibrin based gelatinous coat over repairing rat gastric epithelium after acute ethanol damage: interaction with adherent mucus. Gut 28, 835-843.
37. Spiller RC (1994) Pharmacology of dietary fibre. Pharmacology and Therapeutics 62, 407-427.
38. Stephen AM & Cummings JH (1979) Water-holding by dietary fibre in vitro and its relationship to faecal output in man. Gut 20, 722-729.
39. Strombeck DR & Harrold D (1974) Binding of cholera toxin to mucins and inhibition by gastric mucins. Infection and Immunity 10, 1266-1272.
40. UK Parliament (1986) Animals (Scientific Procedures) Act. London: H.M. Stationery Office.
41. van der Waaij D (1996) The nature and function of interactions between the intestinal microflora and the host. In Inside Story: A Guide to the Intestinal Microflora, pp. 17-23. Place of publication: Yakult, Nederland B. V.
42. Wallace JL (1989) Gastric resistance to acid: is the 'mucusbicarbonate barrier' functionally redundant? American Journal of Physiology 256, G31-G38.
43. Waynforth HB & Flecknall PA (1992) Experimental and Surgical Technique in the Rat. London: Academic Press Ltd.