Rapid identification of differentially expressed genes by combination of SSH and MOS

Laboratory Investigation

Volumn 83, Issue 5, 2003, Pages 759-761

  Boengler, Kerstin ^a,b   Pipp, Frederic ^a   Schaper, Wolfgang ^a   Deindl, Elisabeth ^a  

^a M P Inst for Physiol/Clin Res   (Germany)

^b MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ANGIOGENESIS; ARTICLE; DIFFERENTIAL DISPLAY; DNA MICROARRAY; GENE AMPLIFICATION; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC PROCEDURES; GENETIC SCREENING; GENETIC TRANSCRIPTION; MIRROR ORIENTATION SELECTION; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; SUPPRESSION SUBTRACTIVE HYBRIDIZATION;

EID: 0037799614     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.LAB.0000069520.16101.73     Document Type: Article

References (6)

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3. Chomczynski P, and Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 162:156-159.
4. Diatchenko L, Lau Y-F, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskayqa N, Sverdlov ED, and Siebert PD (1996). Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA 93:6025-6030.
5. Ito WD, Arras M, Scholz D, Winkler B, Htun P, and Schaper W (1997). Angiogenesis but not collateral artery growth is associated with ischemia after femoral artery occlusion. Am J Physiol 273:H1255-H1265.
6. Rebrikov DV, Britanova OV, Gurskaya NG, Lukyanov KA, Tarabykin VS, and Lukyanov SA (2000). Mirror orientation selection (MOS): A method for eliminating false positive clones from libraries generated by suppression subtractive hybridization. Nucl Acids Res 28(20):e90.