Detection of shiga-like toxin-producing Escherichia coli by multiplex PCR

Biologia - Section Cellular and Molecular Biology

Volumn 56, Issue SUPPL. 9, 2001, Pages 65-68

  Liptáková, Adriána ^a   Sehnálková, Hedviga ^c   Sabol, Marián ^a   Kerestešová, Hana ^a   Rosocha, Ján ^a   Kotulová, Daniela ^b   Siegfried, Leonard ^a  

^a P J AFÁRIK UNIVERSITY   (Slovakia)

^b Bratislava   (Slovakia)

^c NONE

Author keywords

HUS; Multiplex PCR; Shiga like toxins; STEC

Indexed keywords

EID: 0037783871     PISSN: 13356399     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. BEUTIN, L., MONTENEGRO, L. M. A., ORSKOV, I., ORSKOV, F., PRADA, J., ZIMMERMANN, S. & STEPHAN, R. 1989. Close association of verotoxin (Shiga-like toxin) production with enterohaemolysin production in strains of Escherichia coli. J. Clin. Microbiol. 27: 2559-2564.
2. BEUTIN, L., ZIMMERMANN S. & GLEIER, K. 1998. Human infections with shiga toxin-producing Escherichia coli other than serogroup O157 in Germany. Emerg. Infect. Dis. 4: 635-638.
3. BIELASZEWSKA, M., SCHMIDT, H., LIESEGANG, A., PRAGER, R., RABSCH, W., TSCHAPE, H., ČÍŽEK, A., JANDA, J., BLÁHOVÁ, J. & KARCH, H. 2000. Cattle can be a reservoir of sorbitol-fermenting Shiga toxin-producing Escherichia coli O157: H-strains and a source of human diseases. J. Clin. Microbiol. 38: 3470-3473.
4. CAPRIOLI, A. & TOZZI, A. E. 1998. Epidemiology of Shiga toxin-producing Escherichia coli infections in continental Europe, pp. 38-48. In: KAPER, J. B. &: O'BRIEN, A. D. (eds) Escherichia coli O157:H7 and Other Shiga Toxin-Producing Escherichia coli Strains, American Society for Microbiology, Washington, D.C.
5. ČÍŽEK, A., ALEXA, P., LITERÁK, I., HAMRÍK, J., NOVÁK, P. & SMOLA, J. 1999. Shiga toxin-producing Escherichia coli O157 in feedlot cattle and Norwegian rats from a large-scale farm. Lett. Appl. Microbiol. 28: 435-439.
6. KIMMITT, P. T., HARWOOD, C. R. & BARER, M. R. 2000. Toxin gene expression by Shiga toxin-producing Escherichia coli: the role of antibiotics and the bacterial SOS response. Emerg. Infect. Dis. 6: 458-465.
7. PARRY, S. M. & SALMON, R. L. 1998. Sporadic STEC O157 infection: secondary household transmission in Wales. Emerg. Infect. Dis. 4: 657-660.
8. O157. J. Clin. Microbiol. 36: 598-602.
9. PATON, J. C. & PATON, A. W. 1998b. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11: 450-479.
10. PATON, A. W., PATON, J. C., GOLDWATER, P. N. & MANNING, P. A. 1993. Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction. J. Clin. Microbiol. 31: 1063-3067.
11. RAMOTAR, K., WALDHART, B., CHURCH, D., SZUMSKI, R. & LOUIE, T. J. 1995. Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR. J. Clin. Microbiol. 33: 519-524.
12. SCHWAIGER, M., GRIF, K., PIERARD, D., KARCH, H. & ALLERBERGER, E. 1999. Shiga toxin-producing Escherichia coli was the third most frequent bacterial cause of diarrhea in Austria during July and August of 1998. Clin. Microbiol. Infect. 5: 645-647.
13. SIEGFRIED, L., KMETOVA, M., PUZOVA, H., ROSOCHA, J., LIPTAKOVA, A., SEHNALKOVA, H. 1999. Characteristics of Escherichia coli strains isolated in cases of hemolytic uremic syndrome. Transactions of the Universities 5: 67-69.
14. SOBIESZCZANSKA, B. M., GRYKO, R., MALEK, C. W. 2000. Isolation of verotoxigenic strains of Escherichia coli O26 in Poland. Clin. Microbiol. Infect. 6: 227-229.