Purification of fusion ferritin from recombinant E. coli using two-step sonications

Biotechnology Letters

Volumn 25, Issue 12, 2003, Pages 993-996

  Huh, Yun Suk ^a   Kim, In Ho ^a  

^a Chungnam National University   (South Korea)

Author keywords

Ferritin; Gel filtration chromatography; Purification; Sonication

Indexed keywords

FERRITIN; HYBRID PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CLASSIFICATION; ESCHERICHIA COLI; EVALUATION; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR WEIGHT; PILOT STUDY; RADIATION EXPOSURE; SPECIES DIFFERENCE; ULTRASOUND;

ESCHERICHIA COLI; FERRITINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MOLECULAR WEIGHT; PILOT PROJECTS; RECOMBINANT FUSION PROTEINS; SONICATION; SPECIES SPECIFICITY;

ESCHERICHIA COLI;

EID: 0037742406     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1024005408387     Document Type: Article

References (15)

1. Arosio P, Levi S (2001) Ferritin, iron homeostasis, and oxidative damage. Free Radical Bio. Med. 33: 457-463.
2. Barcelo F, Miralles F, Arean CO (1997) Purification and characterization of ferritin from alfalfa seeds. J. Inorg. Biochem. 66: 23-27.
3. Bauminger ER, Treffey A, Hudson AJ, Hechel D, Hudson NW, Andrews SC, Levi S, Nowik L, Arosio P, Guest JR, Harrison PM (1994) Iron incorporation into ferritins: evidence for the transfer of monomeric Fe(III) between ferritin molecules and for the formation of an unusual mineral in the ferritin of Escherichia coli. Biochem. J. 302: 813-820.
4. Bauminger ER, Treffry A, Quail MA, Zhao Z, Nowik I, Harrison PM (1999) Stages in iron storage in the ferritin of Escherichia coli(EcFtnA): analysis of Mossbauer spectra reveals a new intermediate. Biochemistry-US 385: 7791-7802.
5. Greetha C, Deshpande V (1999) Purification and characterization of fish liver ferritins. Comp. Biochem. Phys. B123: 285-294.
6. Hudson, AJ, Andrews SC, Hawkins C, Williams JM, Izuhara M, Meldrum FC, Mann S, Harrison PM, Guest JR (1993) Overproduction, purification and characterization of the Escherichia coli ferritin. Eur. J. Biochem. 218: 985-995.
7. Kim SW, Kim YH, Lee JW (2001) Thermal stability of human ferritin: concentration dependence and enhanced stability of an N-terminal fusion mutant. Biochem. Biophys. Res. Commun. 289: 125-129.
8. Laulhere JP, Laboure AM, Briar JF (1989) Mechanism of the transition from plant ferritin to phytosiderin. J. Biol. Chem. 264: 3629-3635.
9. Levi S, Luzzago A, Cesareni G, Cozzi A, Franceschinelli F, Al-bertini A, Arosio P (1988) Mechanism of ferritin iron uptake: activity of the H-chain and deletion mapping of the ferro-oxidase site. A study of iron uptake and ferro-oxidase activity of human liver, recombinant H-chain ferritins, and of two H-chain deletion mutant. J. Biol. Chem. 263: 18086-18092.
10. Levi S, Yewdall SJ, Harrison PM, Santambrogio P, Cozzi A, Rovida E, Albertini A, Arosio P (1992) Evidence that H- and L-chains have co-operative roles in the iron-uptake mechanism of human ferritin. Biochem. J. 288: 591-596.
11. H) from recombinant E. coli. Kor. J. Biotechnol. Bioeng. 17: 446-451.
12. Santambrogio P, Levi S, Cozzi A, Rovida E, Albertini A, Arosio P (1993) Production and characterization of recombinant heteropolymers of human ferritin H and L Chains. J. Biol. Chem. 15: 12744-12748.
13. Sczekan SR, Joshi JG (1987) Isolation and characterization of ferritin from soyabeans (Glycine max). J. Biol. Chem. 262: 3780-3788.
14. Theil EC (1987) Ferritin: structure, gene regulation and cellular function in animals, plants and micro organisims. Annu. Rev. Biochem. 56: 289-315.
15. Vondarl M, Harrison PM, Bottke W (1994) Expression in Escherichia coli of a secreted invertebrate ferritin. Eur. J. Biochem. 222: 367-376.